Circular RNA hsa_circ_0003204 promotes cervical cancer cell proliferation, migration, and invasion by regulating MAPK pathway.

Cervical cancer (CC) is the second most common malignancy in women worldwide. The mechanism underlying CC development remains unclear. Recently, Circular RNAs (circRNAs)have attracted attention because of its role in tumorigenesis.

[Analysis of Human Herpes Viruses-Activated Infection Spectra in Patients with Various Immunodeficiencies].

Objective: To study the epidemiologic characteristics of human herpes virus (HHV) activated infection in the diseases of blood system and patients received allo-HSCT by statistically analyzing the screening results of 8 human herpes viruses (HHVs) of 4164 patients in Hebei Yanda LU Dao-Pei Hospital from 2012 to 2017.

Methods: PCR was used to screen 8 HHVs.

Results: Two thousand and fifty-two patients (49.

Ultrastructure Characteristics of Different Chinese Medicine Syndromes of Helicobacter pylori-Correlated Gastric Diseases.

Objective: To explore the ultrastructure characteristics of patients with dampness-heat of Pi (Spleen)-Wei (Stomach) syndrome (DHPW) and Pi-qi deficiency syndrome (PQD), both of which are Helicobacter pylori (Hp)-correlated gastric diseases (HPCG), and implicate a helpful hint for the clinical microcosmic syndrome differentiation.

Methods: Fourteen gastric mucosa samples from 6 chronic gastritis (CG) and 6 active peptic ulcer (including 8 DHPW, 4 PQD) as well as 2 healthy volunteers were collected and tested for Hp infection. The ultrastructure of gastric mucosa was observed under the transmission electron microscope (TEM).

[Link between sortase A function and cariogenicity of Streptococcus mutans: a preliminary metabolomics analysis].

Objective: This study intends to explore the mechanism underlying the support of sortase A (SrtA) of the cariogenicity of Streptococcus mutans (S. mutans).

Methods: We performed a metabonomics study based on ¹H nuclear magnetic resonance spectroscopy (NMR), in which we compared the extracellular metabolites of wild-type S.

[Resource investigation of wild Codonopsis pilosula in Tanchang county of Gansu].

Tanchang county is the distribution of wild medicinal plant resource-rich region, in order to ascertain Tanchang county Codonopsis pilosula wild resources and reserves of the status quo, according to the fourth national Chinese medicine resources survey technology solutions, using sets of plots and investigating combined route survey method, the county wild C. pilosula var. modesta and C.

Comparison of cell proliferation, apoptosis, cellular morphology and ultrastructure between human umbilical cord and placenta-derived mesenchymal stem cells.

Research in mesenchymal stem cells (MSCs) is mainly focused on applications for treatments of brain and spinal cord injury as well as mechanisms underlying effects of MSCs. However, due to numerous limitations, there is little information on selection of appropriate sources of MSCs for transplantation in clinical applications. Therefore, in this study we compared various properties of human umbilical cord-derived MSCs (HUCMSCs) with human placenta-derived MSCs (HPDMSCs), including cell proliferation, apoptosis, cellular morphology, ultrastructure, and their ability to secrete various growth factors (i.

Creation of a female rabbit model for intrauterine adhesions using mechanical and infectious injury.

Background: Intrauterine adhesions (IUA) are associated with secondary amenorrhea, infertility, and recurrent pregnancy loss. An IUA animal model would contribute to research on the pathogenesis and pathologic changes of IUA and the exploration of new treatments.

Materials And Methods: Eighty female rabbits were randomly divided into five groups: mechanical injury (16), infectious injury (16), dual injury (16), experimental control (16), and normal (16).

Prognostic implications of microRNA-100 and its functional roles in human epithelial ovarian cancer.

Dysregulation of microRNAs (miRNAs) has been found to be associated with a variety of diseases, including epithelial ovarian cancer (EOC). Recently, miR-100 was reported to be downregulated in human ovarian carcinoma, however, the clinical significance and functional roles of miR-100 expression in human EOC are unclear. TaqMan real-time quantitative RT-PCR assay was performed to detect the expression of miR-100 in 98 EOC tissues and 15 adjacent normal epithelial tissues.

[Biological features and ultrastructure of human umbilical cord mesenchymal stem cells].

Objective: To isolate and culture human umbilical cord mesenchymal stem cells (MSCs) and explore their biological features and ultrastructure.

Methods: After isolating MSCs from the human umbilical cord, the proliferation, cycle, and apoptosis were observed. The cell ultrastructure was observed under transmission electron microscope.

PKH26 as a fluorescent label for live human umbilical mesenchymal stem cells.

To determine whether PKH26 labeling affects the morphologies, phenotypes, proliferation, and secretion abilities of human umbilical mesenchymal stromal cells (HUMSCs) were investigated. Isolated HUMSCs were labeled with PKH26, and cell morphology was observed under microscope. Cell cycle, apoptotic cell death, expression of PKH26, and the proliferation rate were evaluated.

[Inhibitory effect of short hairpin RNA targeting survivin gene on human ectopic endometrial cells].

Objective: To construct a lentiviral expression vector for short hairpin RNA (shRNA) of human survivin gene, and assess its gene silencing effect in human ectopic endometrial cells.

Methods: Human survivin gene shRNA sequence was designed using a software available on-line. The synthesized shRNA sequence was cloned into the pGCL-GFP vector to construct LV-survivin shRNA, which was confirmed by PCR and DNA sequence analysis.

[Protective effect of estrogen against chemotherapy-induced ovarian damage in rats].

Objective: To investigate the protective effect of estrogen against cyclophosphamide (CTX)-induced ovarian failure in female rats.

Methods: Sixty female Wistar rats (2-3 months old) were randomized into 4 groups to receive treatments with normal saline, CTX, estradiol (E2) or CTX+E2. Serum estradiol and follicle-stimulating hormone (FSH) concentrations were measured regularly in the 4 groups, and the weight of the ovary and uterus, follicle number and mean diameter of the follicles were compared.

[Small interfering RNA targeting NF-kappaB Inhibits human umbilical venous endothelial cells migration].

Aim: To investigate the effect of small interfering RNA(siRNA) targeting NF-kappaB on migration ability of human umbilical venous endothelial cells.

Methods: EAhy926 cells (Human umbilical vein endothelial cell line) were cultured with stimulation of vascular endothelial growth factor (VEGF), and then were transfected with NF-kappaB -siRNA (40 pmol/L) by using catiotic liposome lipofectamine(TM); 2000 as the transfecting agent. Cells transfected with negative control-siRNA (40 pmol/L), empty Liposome and nothing as control.

[Small interference RNA targeting nuclear factor-kappaB inhibits endometriotic angiogenesis in chick embryo chorioallantocic membrane].

Objective: To investigate the effect of small interference RNA (siRNA) targeting nuclear factor-kappaB (NF-kappaB) on endometriosis.

Method: The eutopic endometrium of women with endometriosis were transplanted into the nonvascular region of 8-day-old chicken embryo chorioallantocic membrane (CAM), and the effects of NF-kappaB p65 siRNA on the vascularization and endometriotic lesion formation were tested with proper controls.

Results: Transplantation of the endometrium onto the CAM resulted in a strong angiogenic response in the chicken tissue.

[Construction of a lentiviral vector carrying human bcl-2 gene and its expression in human ovarian granulosa cells].

Objective: To construct a lentiviral vector carrying human bcl-2 gene and investigate its expression in human ovarian granulosa cells (GCs).

Methods: Human bcl-2 gene was amplified from the plasmid pCMV-SPORT6 using PCR and subcloned into the lentiviral vector pGC-FU to construct the lentiviral expression vector pGC-FU- bcl-2. The bcl-2 gene insert was confirmed by restriction enzyme digestion and sequencing.

[Expression of vascular endothelial growth factor in nude mouse models of human endometriosis].

Objective: To investigate the expression of vascular endothelial growth factor (VEGF) in eutopic and ectopic endometrium of in nude mice models of endometriosis and understand the role of VEGF in the development of endometriosis.

Methods: Nude mouse models of endometriosis were established by subcutaneous implantation and abdominal injection of human endometrium xenograft from patients with endometriosis. The morphology of the foci was observed microscopically, and VEGF expression was measured with immunohistochemistry.

[Green fluorescent protein gene transfection mediated by recombinant adenovirus vector to rat bone marrow mesenchymal stem cells].

Objective: To investigate the efficient transfection of green fluorescent protein gene (GFP) mediated by recombinant adenovirus vector(Ad-GFP) to rat bone marrow mesenchymal stem cells (MSCs) in vitro.

Methods: Wistar rat bone marrow-derived MSCs were separated and purified in vitro by Percoll density gradient centrifugation combined with adherent cell culture followed by identification with flow cytometry. MSCs infected by Ad-GFP were observed and the transfection efficiency was assessed by fluorescence microscope.

[Construction of a recombinant adenovirus carrying endostatin gene].

Objective: To construct a recombinant adenovirus carrying human endostatin gene with AdEasy system.

Methods: Endostatin gene fragment was amplified from Pshuttle-Endostatin plasmid with PCR and subcloned into the pAdTrack-CMV shuttle vector. The resultant plasimid was cotransduced into E.

[Effect of gene polymorphism of TNF-beta on the concentration of TNF in serum of patient with endometriosis].

Objective: To determine the polymorphism in +252 site of tumor necrosis factor-beta(TNF-beta) gene in patients with or without endometriosis, to evaluate the levels of TNF-alpha and TNF-beta in the serum with or without endometriosis, to explore the relation between polymorphism of TNF-beta gene and the genetic susceptibility of endometriosis, and to explore the pathogenic mechanism of endometriosis at gene level.

Methods: By polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method, polymorphism on +252 site of TNF-beta gene was measured in 82 patients with endometriosis (the endometriosis group) and 80 patients without endometriosis (the control group). With the sandwich-enzyme-linked immunosorbent assay (ELISA), the levels of TNF-alpha and TNF-beta in the serum of the two groups were determined.

[Expression of hypoxia-inducible factor-1alpha in endometriosis].

Objective: To detect the expression of hypoxia-inducible factor-1alpha(HIF-1alpha) in endometriosis and explore the possible role of HIF-1alpha in the pathogenesis of endometriosis.

Methods: Immunohistochemistry was performed to examine the expression of HIF-1alpha in 20 normal endometrium, 20 ectopic endometrium and 68 eutopic endometrium specimens from 68 endometriosis patients, and the results were analyzed statistically.

Results: The expression of HIF-1alpha was significantly increased in ectopic endometrium than in normal endometrium (P<0.

[Construction of a recombinant adenoviral vector expressing human endostatin].

Objective: To construct the recombinant adenovirus vector expressing human endostatin.

Methods: Human endostatin gene extracted from pGEM-T Easy vector containing the target gene fragment was successfully amplified using PCR and cloned into pShuttle2 vector. The target gene was subcloned into an adenovirus vector and the resulted recombinant adenovirus (Ad-hEndo) was linearized before transfected into HEK 293 packaging cells.

[Association of tumor necrosis factors-beta gene polymorphism with endometriosis in women in Guangdong Province].

Objective: To study the polymorphism of +252 site in intron 1 of tumor necrosis factor (TNF)-beta gene in relation to genetic susceptibility of endometriosis.

Methods: Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was employed to detect the polymorphism of +252 site in intron 1 of TNF-beta gene in 82 Chinese Han patients with endometriosis in Guangdong Province and 80 Han patients without endometriosis (control group), and the relation between TNF gene polymorphism and the risk of endometriosis was analyzed.

Results: The +252 site of TNF-beta allele and genotype distribution showed significant difference between endometriosis and control groups (Chi2=6.

[Recombinant human endostatin induces apoptosis of human umbilical venous endothelial cells].

To investigate the effects of human endostatin on proliferation of human umbilical venous endothelial cells, the apoptosis of the endothelial cells treated with recombinant human endostatin was examined by fluorescence method and the cell proliferation activity analyzed by MTT assay. The results showed that human endostatin inhibited the proliferation of the endothelial cells with ED(50) of 550 ng/ml, and the inhibitory effect was enhanced with the increase of endostatin concentration, suggesting a dose-dependent inhibitory effect of human endostatin on endothelial cell proliferation. The mechanism of antiangiogenesis induced by human endostatin is related to the inhibition of cell proliferation and induction of apoptosis of the endothelial cells.