[Analysis on tumor related gene expression profiles in benzene poisoning using cDNA microarray].

Objective: To detect target genes for further study by way of analyzing the gene expression profiles of benzene poisoning by using cDNA microarray.

Methods: Peripheral mononuclear cells were isolated from seven patients with benzene poisoning of different degrees, and sevene age-and sex-matched normal subjects. Total RNA was extracted and purified, followed by revese transcription to cDNAs with concomitant incorporation of fluorescent dCTP (Cy3 or Cy5).

[Detection of apoptosis genes differentially expressed in patients with different degrees of benzene poisoning by cDNA microarray].

Objective: To investigate the differential expression of apoptosis genes in patients with different degrees of benzene poisoning by cDNA microarray.

Methods: Peripheral mononuclear cells were isolated from seven patients with benzene poisoning of different degrees (suspected 1 case, moderate 2, severe 2), and seven age and sex matched normal control subjects. Total RNA was extracted and purified, followed by reverse transcription to cDNAs with concomitant incorporation of fluorescent dCTP (Cy3 or Cy5).

[Association of p53, PCNA expression and trace element content in esophageal mucosa].

Background & Objective: There were abnormal changes of trace element in esophageal cancer patient's hair and serum in the high risk area. But it was still unknown that the relationship between p53 and proliferating cell nuclear antigen(PCNA) expression and the trace element content in varied esophageal mucosa. This study was designed to probe the relationship of trace element content and p53 mutation, PCNA expression in esophgeal mucosa.

Effect of manganese on heat stress protein synthesis of new-born rats.

Aim: To study the effect of manganese (Mn) on heat stress protein 70 (HSP70) synthesis in the brain and liver of new-born rats whose mother-rats were exposed to Mn.

Methods: 32 female rats were randomly divided into four groups. One group was administrated with physiological saline only as control group, the other three groups were administrated with 7.