[Study on the feasibility of a cell-culture inoculation test in the detection and isolation of street rabies virus].

Objective: Feasibility of using MNA cell-culture inoculation test to detect and isolate the street rabies virus.

Methods: Using MNA cell-culture inoculation test, fluorescent antibody test (FAT) and sandwich ELISA with double-antibodies to detect 33 specimens of street rabies virus, 20 specimens of negative canine brains and 4 specimens of healthy mice brains.

Results: 33 specimens of street rabies virus were positive to the cell-culture inoculation test but the others were negative.

A molecular epidemiological study targeting the glycoprotein gene of rabies virus isolates from China.

A group of 31 rabies viruses (RABVs), recovered primarily from dogs, one deer and one human case, were collected from various areas in China between 1989 and 2006. Complete G gene sequences determined for these isolates indicated identities of nucleotide and amino acid sequences of >or=87% and 93.8%, respectively.