Network pharmacology analysis of the renal protective effects of bracken (Pteridium aquilinum) using experimental data obtained in an avian (quail) hyperuricemia model.

Objective: To investigate the nephroprotective potential of orally administered bracken extract against renal damage in quails, induced by a high-purine diet, to form a foundation for subsequent clinical studies and applications.

Materials And Methods: A mass spectrometry analysis was conducted on the pteridophyte subjected to steam explosion. Network pharmacological methods were then utilized to pinpoint shared targets and pathways, which suggested that has a capability to counteract renal injury.

[Comparative study on serum transitional components of Huatan Jiangqi Capsules in rats under pathological and physiological status].

The differences of transitional components and metabolic processes of Huatan Jiangqi Capsules(HTJQ) in rats under normal physiological and pathological conditions of COPD were analyzed by UPLC-Q-TOF-MS. The rat COPD model was established by passive smoking and intratracheal instillation of lipopolysaccharide. After the normal and COPD model rats were douched with HTJQ, the blood was collected from hepatic portal vein and the drug-containing serum samples were prepared by methanol precipitation of protein.

(-)-Epigallocatechin-3-gallate encapsulated realgar nanoparticles exhibit enhanced anticancer therapeutic efficacy against acute promyelocytic leukemia.

Realgar and (-)-Epigallocatechin-3-gallate (EGCG) are natural medicines that inhibit cancer cell growth, resulting in inhibition of formation and development of tumors. The anticancer effects of realgar and EGCG were greatly improved following formulation as nanoparticles. EGCG has received increased attention as a drug carrier.

Aminated β-cyclodextrin-grafted FeO-loaded gambogic acid magnetic nanoparticles: preparation, characterization, and biological evaluation.

Based on aminated β-cyclodextrin (6-NH-β-CD)-grafted FeO and gambogic acid (GA) clathrate complexes, a nanoparticle delivery system was developed with the aim to achieve low irritation, strong targeting, and high bioavailability of a gambogic acid magnetic nanopreparation. 6-NH-β-CD grafted onto FeO MNPs was demonstrated by high-resolution transmission electron microscopy, Fourier transform infrared spectroscopy, X-ray diffraction, zeta potential, and magnetic measurements. The average particle size of the FeO@NH-β-CD MNPs was 147.

Similarity Evaluation of Different Origins and Species of Dendrobiums by GC-MS and FTIR Analysis of Polysaccharides.

GC-MS method combined with FTIR techniques by the analysis of polysaccharide was applied to evaluate the similarity between wild (W) and tissue-cultured (TC) Dendrobium huoshanense (DHS), Dendrobium officinale (DO), and Dendrobium moniliforme (DM) as well as 3 wild Dendrobium spp.: Dendrobium henanense (DHN), Dendrobium loddigesii (DL), and Dendrobium crepidatum (DC). Eight monosaccharides involving xylose, arabinose, rhamnose, glucose, mannose, fructose, galactose, and galacturonic acid were identified in the polysaccharide from each Dendrobium sample while the contents of the monosugars varied remarkably across origins and species.

Preparation and Characterization of Folate Targeting Magnetic Nanomedicine Loaded with Gambogenic Acid.

To engineer multifunctional nanomedicines for simultaneous imaging and therapy of cancer cells, we have prepared Gambogenic acid (GNA) loaded folic acid (FA) armed MNPs (FA-GNA-MNPs) to target the folate receptor (FR) positive cancer cells. The FA-GNA-MNPs have been prepared by a facile method, which have been further characterized by SEM, TEM, IR and UV-vis spectra. And the cytotoxicity of FA-GNA-MNPs to HeLa and A549 cells was assessed using the MTT assay.

[Preparation Technique of Tetraploid of Dioscorea zingiberensis Synthetic Seed Based on Embryogenic Callus].

Objective: To provide technical support for industrialization promotion of tetraploid of Dioscorea zingiberensis, the manufacturing method for synthetic seeds of tetraploid of Dioscorea zingiberensis was established and the correlated influential factors were studied.

Methods: By taking embryogenic calluses of tetraploid of Dioscorea zingiberensis as propagation materials, the influential factors such as components of artificial endosperm, seed coats,storage conditions and germination materials on germination and seedling of the synthetic seeds were evaluated.

Results: When 4% alginate +2% CaCl2 + 2% chitosan was served as seed coat materials, and 1/2 MS +0.

The application of biotechnology in medicinal plants breeding research in China.

Breeding is not only an important area of medicinal plants research but also the foundation for the superior varieties acquirement of medicinal plants. The rise of modern biotechnology provides good opportunities and new means for medicinal plants breeding research in China. Biotechnology shows its technical advantages and new development prospects in breeding of new medicinal plants varieties with high and stable yield, good quality, as well as stress-resistance.

[Genetic stability study on autotetraploid plant of Dioscorea zingiberensis].

Objective: To study the genetic stability of autotetraploid plant of Dioscorea zingiberensis.

Methods: The chromosome of root-tip was determined by photomicroscope, and the agronomic characters were observed in the period of stable growth. The protein content was determined and the experiment of protein polyacrylamide gel electrophoresis was carried out.

Germplasm preservation in vitro of Polygonum multiflorum Thunb.

Background: The root of Polygonum multiflorum Thunb. is a common traditional Chinese medicine. In recent years, the wild resources of P.

[Studies on adventitious root induction in vitro and suspension culture of Polygonum multiflorum].

To achieve sustainable resources use of Polygonum multiflorum, adventitious roots were efficiently induced and cultured by suspension culture. In order to obtain optimal medium for induction adventitious roots from the young stems of P. multiflorum, MS medium was optimized by supplementing with different concentrations of sucrose and plant growth substances.

[Study on inducing embryogenic callus of Dioscorea zingiberensis by orthogonal design].

Objective: To select the suitable medium to induce embryogenic callus of Dioscorea zingiberensis.

Methods: Plantlet of Dioscorea zingiberesis in vitro was obtained by using apical meristem as explant. The different parts of the plantlets were cultured to select the best explant used for inducing callus and embryoids.

[Optinization of rapid propagation technique and induction and identification of autotetraploid of Polygonum multiflorum].

Objective: To establish and optimize the rapid propagation system of Polygonum multiflorum, as well as explore method for induction and identification of autotetraploid.

Method: Propagation medium was optimized by orthogonal test. The buds were immersed in colchicine solution with different concentrations for different time to select induction conditions for autotetraploid of P.

15-Meth-oxy-14,15-di-hydro-andranginine.

The title polycyclic alkaloid, C22H26N2O3, an indole derivative obtained from Melodinus yunnanensis, comprises three chiral C atoms and crystallizes as a racemate. Its seven-membered heterocyclic ring has a twisted conformation, with the N atom within the plane of the indole moiety and with two adjacent C atoms deviating in opposite directions from its plane by 0.756 (3) (methyl-ene C) and -0.

[Clinical study on hemangiomas treatment with high-intensity focused ultrasound (60 cases)].

Objective: To explore the clinical effect and appropriate power of high-intensity focused ultrasound (HIFU) in the treatment of infant hemangioma.

Methods: 80 infants with hemangioma were randomly divided into four groups, 20 cases in each group. All cases in group A, B and C were irradiated on the lesion surface with 3-5 mm/s speed for five continuous come-and-goes using HIFU, with frequency 9 MHz, impulse 1 000 Hz and 10% scanning overlap.

Nitrogen level changes the interactions between a native (Scirpus triqueter) and an exotic species (Spartina anglica) in Coastal China.

The exotic species Spartina anglica, introduced from Europe in 1963, has been experiencing a decline in the past decade in coastal China, but the reasons for the decline are still not clear. It is hypothesized that competition with the native species Scirpus triqueter may have played an important role in the decline due to niche overlap in the field. We measured biomass, leaf number and area, asexual reproduction and relative neighborhood effect (RNE) of the two species in both monoculture and mixture under three nitrogen levels (control, low and high).

Generation of autotetraploid plant of ginger (Zingiber officinale Rosc.) and its quality evaluation.

Background: Zingiber officinale Rosc. is not only an important medical plant in China, but also one of the most commonly used plant spices around the world. Early researches in Z.

Tissue culture and generation of autotetraploid plants of Sophora flavescens Aiton.

Background: Sophora flavescens Aiton is an important medicinal plant in China. Early in vitro researches of S. flavescens were focused on callus induction and cell suspension culture, only a few were concerned with in vitro multiplication.

In vitro tetraploid induction and generation of tetraploids from mixoploids in Dioscorea zingiberensis.

This article describes an efficient colchicine-mediated technique for the in vitro induction of tetraploids in Dioscorea zingiberensis and its confirmation by flow cytometry. Buds immersed in 0.2% colchicine solution for 36 hours prior to culture induced as high as 35.

[Clinical analysis of cleft lip and palate repair at the same time on 468 infants].

Objective: To study the feasibility, merit and shortcoming of simultaneously repairing cleft lip and palate during the infancy.

Methods: 468 infants with cleft lip and palate repaired simultaneously in our department from 1995 to 2004 were reviewed retrospectively, as well as the cases repaired three years before were followed up and evaluated in pronunciation.

Results: 468 infants with cleft lip and palate were effectively treated simultaneously, associated with 173 had complications and 1 died after operation.