[Determination of benzo(alpha)pyrene in food with microwave-assisted extraction].

Coupling derivative technique and constant-energy synchronous fluorescence scanning technique, a method of determining benzo[alpha] pyrene in foods by second derivative constant-energy synchronous spectrofluorimetry after microwave-assisted treatment of samples was established using domestic microwave oven. The main factors of influencing the efficiency of microwave extraction were discussed, including the extraction solvent types and amounts, the microwave extraction time, microwave radiation power and cooling time. And the comparison with ultrasonic extraction was made.

A novel method for the rapid detection of benzo(a)pyrene in liquid milk by dimethyl sulfoxide selectively enhanced synchronous fluorescence spectrometry.

Based on the high solubility efficiency and strong fluorescence response of benzo(a)pyrene (BaP) in dimethyl sulfoxide in combination with the high-performance derivative constant-energy synchronous fluorescence spectroscopic (DCESFS) technique, a simple, sensitive and economic method was developed for the determination of BaP in liquid milk. This method comprises ultrasound-assisted solvent extraction, solvent replacement and DCESFS detection. No saponification or other tedious clean-up procedures were needed.

[Study of three ciguatera fish poisoning cases in Xiamen city, in 2005].

Objective: To find out the reason of three ciguatera fish poisoning cases in Xiamen in 2005 and identify the fish species.

Methods: The grouper implicated in food poisoning and seven other coral reef fishes collected from market were tested by mice bioassay and ciguatoxin-test kit. The mtDNA was extracted from toxic grouper meat, and Cty b gene segment was amplified and the PCR products were sequenced.

[Determination of migration level of benzo(a) pyrene from paper cups into food simulants].

This paper quantitatively determined the migration level of benzo(a)pyrene (BaP) from paper cups into aqueous food simulants or fatty stimulants, under different conditions by second derivative constant-energy synchronous fluorescence spectrometry (DCESFS), which proved to be a method with high sensitivity and spectral resolution. The influences of food simulants, time and temperature on the relative migration percent(%) of benzo(a) pyrene were discussed. Results showed that the recoveries of this method ranged from 89.

A novel synchronous fluorescence spectroscopic approach for the rapid determination of three polycyclic aromatic hydrocarbons in tea with simple microwave-assisted pretreatment of sample.

Many polycyclic aromatic hydrocarbons (PAHs) are carcinogenic, and some have been reported to be present in tea. People can be exposed to PAHs through tea consumption. Therefore, there is real importance for the determination of PAHs in tea.

[Simultaneous determination of PAHs in food by low-temperature constant-energy synchronous fluorescence spectrometry].

The authors developed a rapid analytical method that could determine the PAHs in food samples simultaneously by the coupled approach of Shpol' skii spectroscopy and constant-energy synchronous fluorescence scanning technique. Low-fat food samples, which were just extracted with octane and high-fat food samples pre-processed with saponification and extracting, could be analyzed immediately. The recoveries ranged from 80.

Highly sensitive and selective spectrofluorimetric approach for the rapid determination of trace benzo[α]pyrene in drinking water and in solutions leached from disposable paper cups.

Based on the excellent band narrowing and background suppressing features of second-derivative constant-energy synchronous spectrofluorimetry with a Δ⁻υ value of 1400 cm(-1) , the strong fluorescence intensity for benzo[α]pyrene (BaP) obtained in dichloromethane and the use of standard addition method, a highly sensitive and selective approach for the quantitative determination of trace amount of BaP in drinking water has been established in this study. The detection and quantification limits were 0.11 and 0.

[Benzo (a) pyrene residue surveillance in retail food in the city of Xiamen, China: report from 121 samples].

Objective: To investigate Benzo (a) pyrene (BaP) residue in retail food of Xiamen.

Methods: BaP residue in 121 retail food samples collected from Xiamen were determined by a rapid BaP detector based on derivative constant-energy synchronous fluorescence technique.

Results: BaP was detected in 84.

A simple and rapid fluorimetric method for simultaneous determination of protoporphyrin IX and zinc protoporphyrin IX in whole blood.

Derivative variable-angle synchronous fluorescence (DVASF) spectrometry improves the spectral resolution and selectivity of the fluorescence method. The feasibility of DVASF spectrometry for the simultaneous determination of protoporphyrin IX (PP) and zinc protoporphyrin IX (ZnPP) was investigated. PP and ZnPP were distinguished from each other simultaneously and rapidly by the DVASF method.