A Prospective Randomized Controlled Study of Ultrasound-Guided Rectus Sheath Block for Pain Management in Laparoscopic Umbilical Hernia Repair with Intraperitoneal Onlay Mesh.

Objective: To evaluate the clinical efficacy and safety of ultrasound-guided rectus sheath block (RSB) in laparoscopic umbilical hernia repair with intraperitoneal onlay mesh (IPOM).

Methods: A total of 139 patients scheduled for laparoscopic umbilical hernia repair with IPOM were selected and randomly assigned to either the group receiving general anesthesia combined with bilateral rectus sheath block (Group GR, 71 patients) or the group receiving general anesthesia alone (Group G, 68 patients). We monitored the patients' heart rate (HR) and mean arterial pressure (MAP) at four time points: before anesthesia induction (T1), at the start of surgery (T2), during mesh fixation (T3), and upon removal of the laryngeal mask (T4).

Interpreting Variants of Uncertain Significance in PCD: Abnormal Splicing Caused by a Missense Variant of DNAAF3.

Background: Primary ciliary dyskinesia (PCD) is a rare autosomal recessive disorder characterized by dysfunction of motile cilia. While approximately 50 genes have been identified, around 25% of PCD patients remain genetically unexplained; elucidating the pathogenicity of specific variants remains a challenge.

Methods: Whole exome sequencing (WES) and Sanger sequencing were conducted to identify potential pathogenic variants of PCD.

Porphyrin-Based Metal-Organic Framework Photocatalysts: Structure, Mechanism and Applications.

In recent years, porphyrins have been frequently reported as photocatalysts due to their fascinating photochemical properties. However, porphyrins have the same shortcomings as other homogeneous photocatalysts, such as poor stability and difficulty in recovering. To solve this problem, it is a good strategy to form a porphyrin-based metal-organic framework (PMOF) by modifying porphyrin functional groups and adding metals as nodes to connect and control the arrangement of porphyrins.

Improved split prime editors enable efficient in vivo genome editing.

Efficient prime editor (PE) delivery in vivo is critical for realizing its full potential in disease modeling and therapeutic correction. Although PE has been divided into two halves and delivered using dual adeno-associated viruses (AAVs), the editing efficiency at different gene loci varies among split sites. Furthermore, efficient split sites within Cas9 nickase (Cas9n) are limited.

Asparagine endopeptidase regulates lysosome homeostasis via modulating endomembrane phosphoinositide composition.

Asparagine endopeptidase (AEP) is ubiquitously expressed in both physiological and pathological contexts, yet its precise role and functional mechanism in breast cancer remain elusive. Here, we identified increased AEP expression in breast cancer tissues, which correlated with poorer survival rates and a propensity for lung metastasis among breast cancer patients. Loss of AEP impaired colony formation by breast cancer cells in vitro and suppressed lung metastasis in mice.

Multi-ancestry Genome Wide Association Study Meta-analysis of Non-syndromic Orofacial Clefts.

Non-syndromic orofacial clefts (NSOC) are common craniofacial birth defects, and result from both genetic and environmental factors. NSOC include three major sub-phenotypes: non-syndromic cleft lip with palate (NSCLP), non-syndromic cleft lip only (NSCLO) and non-syndromic cleft palate only (NSCPO), NSCLP and NSCLO are also sometimes grouped as non-syndromic cleft lip with or without cleft palate (NSCL/P) based on epidemiology. Currently known loci only explain a limited proportion of the heritability of NSOC.

Amino modifications exacerbate the developmental abnormalities of polystyrene microplastics via mitochondria-mediated apoptosis pathway in zebrafish larvae.

Microplastics (MPs) are ubiquitous in the environment and have been identified as a potential threat to ecosystems. However, the mechanisms of toxicity of modified MPs remain unknown. This study investigated the developmental toxicity of amino-modified polystyrene microplastics (PS-NH) with environmentally relevant concentrations ranging from 0.

PhPO-Modulated Kdo Glycosidation for Stereoselective Synthesis of β-Kdo-Containing Disaccharides.

A PhPO-modulated β-selective Kdo glycosidation approach is developed for the stereoselective synthesis of β-Kdo glycosides. With the readily available per--acetylated Kdo ynenoate as the donor, the glycosylation with a series of alcohols in the presence of PhPAuOTf and PhPO in toluene at low temperatures afforded the desired Kdo glycosides with good to excellent β-selectivities. Furthermore, the PhPO-modulated approach was effectively applied to the synthesis of β-(2→4)- and β-(2→8)-linked Kdo-Kdo disaccharides for further biological studies.

In-situ oxidative polymerization of dopamine triggered by CuO in acidic condition and application in "turn-off" sensing.

Dopamine (DA) is a key neurotransmitter whose concentration affects various neurological disorders. Unlike previous methods that synthesize non-fluorescent polydopamine (NFL-PDA) under alkaline conditions, this study introduces a novel "turn-off" sensing method for DA using NFL-PDA synthesized through a unique reaction pathway. In our approach, CuO nanodots, created via a simple reduction method, catalyze the formation of hydroxyl radicals (•OH) in acidic conditions, triggering the oxidative polymerization of DA into NFL-PDA.

Enzyme-free and highly sensitive detection of human epidermal growth factor receptor-2 based on MNAzyme signal amplification in breast cancer.

As a common cancer biomarker, human epidermal growth factor receptor-2 (HER2) is highly expressed in breast cancer. Consequently, developing a simple and accurate HER2 sensing platform is of great significance for early diagnosis and treatment of breast cancer. Herein, we developed a rapid enzyme-free fluorescent assay biosensor based on MNAzyme signal amplification for breast cancer biomarker, HER2.

High-Quality Subsurface Construction of Perovskite Film for Efficient and Stable Solar Cells.

The subsurface of perovskite (PVK) triggers non-radiative recombination and initiates film degradation due to the impurities and defects. This study investigates the limitations of the conventional surface post-treatment and proposes an innovative pre-treatment strategy to achieve complete impurity elimination and defect passivation of the subsurface. The considerable activity of unannealed PVK films provides a sufficient basis for effective subsurface modification.

Construction of an Anode Material for Sodium-Ion Batteries with an Ultrastable Structure.

The reserves of sodium resources are much larger than those of lithium resources, and they are widely distributed and easy to produce and can be widely used in photovoltaic energy storage and other industries on the premise of this advantage. However, how to produce hard carbon anodes at a low cost for the preparation of energy storage materials requires continuous exploration and experimentation to find the optimal solution. Here, we used waste sour date shell biomass as a precursor for a hard carbon anode obtained by simple acid treatment and two pyrolyses.

Association of Multiple Urinary Phthalates Metabolites with Diabetes Risk in Elderly Population.

As a common environmental endocrine disruptor, phthalate exposure could affect the diabetes risk. However, it remains unclear whether phthalate exposure in the elderly population alters diabetes risk. We conducted a cross-sectional survey to explore the effect of urinary phthalate metabolites on diabetes in the elderly.

Compound heterozygous RYR1-RM mouse model reveals disease pathomechanisms and muscle adaptations to promote postnatal survival.

Pathogenic variants in the type I ryanodine receptor (RYR1) result in a wide range of muscle disorders referred to as RYR1-related myopathies (RYR1-RM). We developed the first RYR1-RM mouse model resulting from co-inheritance of two different RYR1 missense alleles (Ryr1 mice). Ryr1 mice exhibit a severe, early onset myopathy characterized by decreased body/muscle mass, muscle weakness, hypotrophy, reduced RYR1 expression, and unexpectedly, incomplete postnatal lethality with a plateau survival of ~50% at 12 weeks of age.

Trimetallic MOF-derived Fe-Mn-Sn oxide heterostructure enabling exceptional catalytic degradation of organic pollutants.

Developing efficient and environmentally benign heterogeneous catalysts that activate peroxymonosulfate (PMS) for the degradation of persistent organic contaminants remains a challenge. Metal-organic frameworks (MOFs)-derived metal oxide catalysts in advanced oxidation processes (AOPs) have received considerable attention research fraternity. Herein, we report an innovative magnetic trimetallic MOF-derived Fe-Mn-Sn oxide heterostructure (FeMnO@Sn) with adjustable morphology, size and Sn content, prepared through an impregnation-calcination strategy.

Rare earth elements in the upstream of Yangtze River Delta: Spatio-temporal distributions, sources and speciations.

With the development of high technology, the environmental pollution caused by rare earth elements (REEs) has gradually aroused social concern, especially the water environment. As a upstream tributary in Xin'an river basin, Fengle river has a decisive impact on the downstream Yangtze River Delta. In this study, the spatial and temporal distributions, sources and the speciations of REEs in surface water of wood, agriculture and industry regions of Fengle River in the two seasons were studied.