Publications by authors named "Hazuki Egashira"
Polyhistidine-tag (His-tag) is a powerful tool for purification of recombinant protein. His-tagged protein can be affinity-purified by using resins immobilizing Ni2+ or anti-His-tag antibodies. However, Ni2+-affinity-purification is prevented by the presence of divalent cations.
View Article and Find Full Text PDFEffective isolation of RNA aptamer through suppression of PCR bias.
Biochem Biophys Res Commun
August 2009
Article Synopsis
- * Traditional SELEX methods can be complex and often require multiple attempts or special equipment to isolate aptamers effectively.
- * The study introduces a new SELEX approach that reduces PCR bias by using RNA transcription for amplification, simplifying the process and allowing the successful isolation of a greater number of RNA aptamers.