Polyphasic characterization and genomic insights into Nocardioides turkmenicus sp. nov. isolated from a desert soil.

Strain KC13, a novel desert-adapted, non-motile, Gram-stain-positive, rod-shaped, aerobic bacterium, was isolated from a soil sample collected from the Karakum Desert, Turkmenistan and characterised by a polyphasic approach. Phylogenetic analysis based on 16S rRNA sequences revealed that strain KC13 was a member of the genus Nocardioides, and formed a distinct cluster with Nocardioides luteus DSM 43366 (99.3% sequence identity), Nocardioides albus DSM 43109 (98.

Genome-based classification of Streptomyces anatolicus sp. nov., an actinobacterium with antimicrobial and cytotoxic activities, and reclassification of Streptomyces nashvillensis as a later heterotypic synonym of Streptomyces tanashiensis.

During the course of isolating novel actinobacteria producing bioactive metabolites, strain BG9H was obtained from an arid soil sample in Erzurum, Turkey. Pairwise sequence comparisons for 16S rRNA gene sequences showed the strain was a member of the genus Streptomyces and it shared the highest 16S rRNA gene sequence identity of 99.7% with Streptomyces huasconensis HST28.

Saccharopolyspora soli sp. nov., isolated from Northern Cyprus soil.

A novel Gram-stain positive, aerobic, non-motile actinobacterium, designated strain K220, was isolated from soil collected from Cape Andreas (Zafer Burnu), Northern Cyprus, and subjected to a polyphasic taxonomic approach. The organism was shown to have phylogenetic, chemotaxonomic, cultural and morphological properties consistent with its classification in the genus Saccharopolyspora. 16S rRNA gene sequence analysis of strain K220 showed that it is closely related to the type strains of Saccharopolyspora maritima 3SS5-12, Saccharopolyspora kobensis JCM 9109 and Saccharopolyspora hirsuta ATCC 27875 with 97.

sp. nov., isolated from the top soil layer on basaltic material in Turkey.

An actinobacterium, designated 14C53, was isolated from a soil sample on basaltic material from Samsun, Turkey. The growth ranges for NaCl concentration and pH of strain 14C53 were quite limited and the growth temperature range of the strain was 20-37 °C, with an optimum at 28 °C. Phylogenetic analysis of 16S rRNA gene sequences revealed that strain 14C53 was most closely related to A8036 (98.

Saccharopolyspora karakumensis sp. nov., Saccharopolyspora elongata sp. nov., Saccharopolyspora aridisoli sp. nov., Saccharopolyspora terrae sp. nov. and their biotechnological potential revealed by genome analysis.

Exploration of unexplored habitats for novel actinobacteria with high bioactivity potential holds great promise in the search for novel entities. During the course of isolation of actinobacteria from desert soils, four actinobacteria, designated as 5K548, 7K502, 16K309 and 16K404, were isolated from the Karakum Desert and their bioactivity potential as well as taxonomic provenances were revealed by comprehensive genome analyses. Pairwise sequence analyses of the 16S rRNA genes indicated that the four strains are representatives of putatively novel taxa within the prolific actinobacterial genus Saccharopolyspora.

Comprehensive genome analysis of a novel actinobacterium with high potential for biotechnological applications, Nonomuraea aridisoli sp. nov., isolated from desert soil.

During a study to isolate such actinobacteria with unique metabolic potential, a novel actinobacterium, designated KC333, was isolated from a soil sample collected from the Karakum Desert, Turkmenistan. The taxonomic position of the strain was investigated using a polyphasic approach. Phylogenetic analysis of the 16S rRNA gene sequence showed that the strain was most closely related to Nonomuraea terrae CH32 (99.

Genomic insight into the clade: reclassification of as a later heterotypic synonym of and as a later heterotypic synonym of .

The taxonomic relationships and genome features of the type strains in the clade, including , , , , , and , were investigated. Type strains of these species shared high 16S rRNA gene sequence similarity to each other. Multilocus sequence analysis (MLSA) based on , , , and genes revealed that and belong to the same species.

sp. nov. and sp. nov., two novel with antimicrobial activity isolated from lake sediment.

Two novel , designated strains YC419 and YC504, were isolated from a sediment sample collected from Lake Yeniçağa, Bolu Province, Turkey. Chemotaxonomic and morphological characteristics of isolates were found to be typical of members of the genus . Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain YC419 was most closely related to NBRC 13094 (99.

sp. nov., isolated from soil.

A novel actinomycete, designated strain HC44, was isolated from a soil sample collected from Hacibektaş, Turkey, and characterized using a polyphasic approach. The strain had morphological characteristics and chemotaxonomic properties identical to those of members of the genus . Phylogenetic analyses based on 16S rRNA gene sequence comparisons revealed that HC44 clustered with members of the genus and the highest 16S rRNA gene sequence similarity values were obtained with NBRC 13094 (97.

Streptomyces boncukensis sp. nov., isolated from saltern soil.

A novel Gram-stain positive, aerobic, non-motile Actinobacterium, designated strain SB3404, was isolated from saltern soil collected from Boncuk Saltern, Sungurlu-Çorum, Turkey, and subjected to a polyphasic taxonomic approach. The organism has shown to have phylogenetic, chemotaxonomic, cultural and morphological properties consistent with its classification in the genus Streptomyces. 16S rRNA gene sequence analysis of strain SB3404 showed that it is closely related to Streptomyces albus NBRC 13014 (97.

sp. nov., isolated from hazelnut orchard soil.

A novel actinobacteria, isolate A7024, was isolated from commercial hazelnut orchard soil sample which was collected at Duzce, West Black Sea region, Turkey. A polyphasic taxonomic study was carried out to determine the status of this isolate. The phylogenetic tree reconstructed using the neighbour-joining algorithm based on 16S rRNA gene sequences indicated that isolate A7024 was positioned within the members of the genus with the highest sequence similarity (97.

Streptomyces boluensis sp. nov., isolated from lake sediment.

A novel Gram-stain positive, aerobic, non-motile actinobacterium, designated strain YC537, was isolated from lake sediment collected from Yenicaga Lake, Bolu, Turkey, and subjected to a polyphasic taxonomic approach. The organism had phylogenetic, chemotaxonomic, cultural and morphological properties consistent with its classification in the genus Streptomyces. 16S rRNA gene sequence analysis of strain YC537 showed that it is closely related to the type strain of Streptomyces ziwulingensis F22 (97.

Shimazuella alba sp. nov. isolated from desert soil and emended description of the genus Shimazuella Park et al. 2007.

A novel, Gram-stain-positive bacterium, designated KC615, was isolated from desert soil which was collected from the Karakum Desert, Turkmenistan. Phylogenetic analysis based on an almost-complete 16S rRNA gene sequence showed that isolate KC615 formed a monophyletic clade with Shimazuella kribbensis KCTC 9933, sharing 98.2% similarity and polyphasic taxonomic studies confirmed the affiliation of the strain to the genus Shimazuella.

Nonomuraea basaltis sp. nov., a siderophore-producing actinobacteria isolated from surface soil of basaltic parent material.

A Gram-stain-positive, aerobic, spore-forming actinobacterial strain, designated 160415, was isolated from a surface soil sample, which was formed on basaltic parent material, collected from Samsun, Turkey. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain 160415 clustered closely with species of the genus Nonomuraea, and showed the highest sequence similarity to Nonomuraea zeae NEAU-ND5, Nonomuraea candida HMC10 and Nonomuraea turkmeniaca DSM 43926 with 99.1%, 98.

Genome-based classification of three novel actinobacteria from the Karakum Desert: sp. nov., sp. nov. and sp. nov.

Three isolates, 5K138, 8K307 and KC603, with typical morphological characteristics of members of the genus were obtained during a study searching for novel actinobacteria with biosynthetic potential from the Karakum Desert. A polyphasic approach was adopted to determine taxonomic affiliations of the strains. The strains showed chemotaxonomic properties consistent with their classification as representing members of the genus such as ll-diaminopimelic acid in the cell wall peptidoglycan, diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol as major polar lipids as well as MK-9(H) as a major menaquinone.

sp. nov., isolated from desert soil and reclassification of as a later heterotypic synonym of .

A novel actinobacterial strain, designated 13K301, was isolated from a soil sample collected from the Karakum Desert, Turkmenistan. The taxonomic position of strain 13K301 was revealed by using a polyphasic approach. On the basis of 16S rRNA gene sequence analysis, strain 13K301 belongs to the genus and had highest sequence similarity to '' S10 (99.

Polyphasic classification of strains isolated from the Karakum Desert and description of sp. nov., sp. nov., sp. nov. and sp. nov.

Five actinobacteria isolates, KC201, KC401, KC310, KC712 and 6K102, were recovered from the Karakum Desert during an investigation of novel actinobacteria with biotechnological potential. A polyphasic approach confirmed the affiliation of the strains to the genus . The strains showed chemotaxonomic and morphological properties consistent with their classification in the genus .

sp. nov., isolated from the Karakum Desert.

An isolate, 13K206, with typical morphological characteristics of the genus was obtained during a study searching for novel actinobacteria with biosynthetic potential from the Karakum Desert. A polyphasic approach was adopted to determine taxonomic affiliation of the strain. The strain showed chemotaxonomical properties consistent with its classification in the genus such as - and 3-OH-Apm in the cell-wall peptidoglycan, xylose in whole-cell hydrolysate and diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol as major polar lipids.

Kribbella turkmenica sp. nov., isolated from the Karakum Desert.

A novel actinobacterial strain, designated 16K104, was isolated from desert soil collected from the Karakum Desert and characterized using a polyphasic approach to clarify its taxonomic position. Strain 16K104 was found to have chemotaxonomic and morphological properties consistent with classification in the genus Kribbella. The strain shared the highest 16S rRNA gene sequence similarity with Kribbella albertanoniae BC640 (99.

Desertiactinospora gelatinilytica gen. nov., sp. nov., a new member of the family Streptosporangiaceae isolated from the Karakum Desert.

A novel, Gram-positive, spore-forming actinomycete, designated strain 7K107, was isolated from a soil sample collected from the Karakum Desert, Turkmenistan. Strain 7K107 forms extensively branched substrate mycelia and aerial mycelia which differentiate into short chains of spores. The novel strain contains meso-diaminopimelic acid as the diagnostic wall amino acid and glucose, galactose, madurose and ribose as whole cell sugars.

Nonomuraea insulae sp. nov., isolated from forest soil.

Strain H2R21, a novel actinobacterium, isolated from a forest soil sample collected from Heybeliada, Istanbul, Turkey, and a polyphasic approach was used for characterisation of the strain. Chemotaxonomic and morphological characterisation of strain H2R21 indicated that it belongs to the genus Nonomuraea. 16S rRNA gene sequence similarity showed that the strain is closely related to Nonomuraea purpurea 1SM4-01 (99.

Amycolatopsis cappadoca sp. nov., isolated from soil.

An Amycolatopsis strain, designated AG28, isolated from a soil sample collected from Acıgöl, Kapadokya, Turkey, was examined using a polyphasic taxonomic approach. Phylogenetic analysis based on an almost-complete 16S rRNA gene sequence showed that the strain is closely related to the type strains of Amycolatopsis deserti GY024 (97.1%), Amycolatopsis taiwanensis 0345 M-7 (96.

Saccharopolyspora hattusasensis sp. nov., isolated from soil.

A Saccharopolyspora strain, designated CR3506, isolated from a soil sample collected from Sungurlu, Corum, Turkey, was examinated using a polyphasic approach. Phylogenetic analysis based on an almost-complete 16S rRNA gene sequence analysis showed that the strain is closely related to the type strains of Saccharopolyspora spinosa NRRL 18395 (99.1%), Saccharopolyspora phatthalungensis NRRL B-24798 (98.

Kribbella sindirgiensis sp. nov. isolated from soil.

A Kribbella strain FSN23 was isolated from soil sample which was collected from Caygoren Dam lakeside located in Sındırgı, Turkey. The isolate was investigated using a polyphasic approach consisting of numeric, chemotaxonomic and molecular analysis. The isolate indicated chemotaxonomic, morphological and phylogenetic properties associated with members of the genus Kribbella.