Publications by authors named "Hayashi Toshihiko"

Article Synopsis
  • Non-triple helical collagen polypeptides (NTHs) are produced in tumor cells and tissues, lacking the typical collagen structure, and were found in various tumor cell lines and human lung cancer samples.
  • NTH production is influenced by serum concentration and hypoxic conditions, and they may play a role in tumor angiogenesis despite the presence of adequate ascorbic acid.
  • The study indicates that NTH production correlates with the accumulation of hypoxia-inducible factor-1α (HIF-1α), but HIF-1α doesn’t directly drive NTH α1(IV) production, suggesting NTHs could be potential therapeutic targets for cancer treatment.
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Background: Reconstruction of the oral modiolus should be considered for facial defects involving the lip and cheek. We have performed free flap reconstruction using a novel technique that includes a vertical and a horizontal sling for full-thickness defects involving the oral commissure.

Patients And Methods: We used a free radial forearm flap and the palmaris longus tendon to correct lip and cheek defects in 6 patients (3 men, 3 women; age, 55-84 years).

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Background: The timing of intervention to treat lymphedema differs among facilities. Understanding differences in the prevalence and severity of lymphedema following different surgical procedures for lymphadenectomy could promote early intervention to treat lymphedema. There is currently little evidence to support the notion that inguino-pelvic lymphadenectomy is associated with greater morbidity than inguinal lymphadenectomy, although it is believed that the difference in the extent of surgery results in a difference in the severity of lymphedema.

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Article Synopsis
  • - The study aimed to establish a method for predicting vascular access difficulty during mechanical thrombectomy by using a specific measurement called the distance from the sternum to the ascending aorta (S-AAD) from chest CT scans.
  • - Researchers analyzed data from 115 patients who underwent mechanical thrombectomy and found that a shorter S-AAD was significantly correlated with longer guiding times, indicating access difficulty.
  • - The findings suggest that a S-AAD of less than 5 mm is a strong predictor of challenging access, which can help clinicians choose better devices and approaches before the intervention.
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  • Pseudo-Kaposi sarcoma (PKS) is a benign vascular condition resembling Kaposi's sarcoma, caused by arteriovenous malformation (AVM) and chronic venous insufficiency, characterized by reddish-brown papules and requiring histopathological differentiation for diagnosis.
  • A case study presents an 83-year-old man with diabetes, who suffered from an ulcer on his second toe due to severe ischemia linked to an AVM and occlusion of the dorsalis pedis artery, resulting in a pathological diagnosis of PKS after skin biopsy.
  • Although the initial ulcer healed, it recurred two years later, leading to infection; attempts to manage blood flow through procedures
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  • The study presents a rare case of symptomatic acute carotid artery occlusion after carotid endarterectomy in a 57-year-old female with a preexisting subclavian artery steal phenomenon.
  • This patient experienced symptoms of cerebral perfusion deficiency due to occlusion of the treated carotid artery the day after surgery.
  • The occlusion was successfully resolved using subclavian artery stenting, leading to improved cerebral blood flow and symptom relief.
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Breast cancer is one of the most common cancers threatening women's health. Our previous study found that silibinin induced the death of MCF-7 and MDA-MB-231 human breast cancer cells. We noticed that silibinin-induced cell damage was accompanied by morphological changes, including the increased cell aspect ratio (cell length/width) and decreased cell area.

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Background: This study aimed to clarify the relationship between primary site and lymphatic drainage pattern for malignant skin tumors in the head and neck region. Malignant melanoma and squamous cell carcinoma in the head and neck region are known to have poor prognosis because of lymph node metastasis. Nevertheless, numerous aspects of lymphatic drainage patterns remain elusive.

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Purpose: Besides comprising scaffolding, extracellular matrix components modulate many biological processes including inflammation and cell differentiation. We previously found precoating cell plates with extracellular matrix collagen I, or its denatured product gelatin, causes aggregation of macrophage-like human lymphoma U937 cells, which are induced to differentiation by phorbol myristate treatment. In the present study, we investigated the influence of gelatin or collagen I precoating on the bacteria phagocytosis in PMA-stimulated U937 cells.

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Collagen I is a major component of extracellular matrix in human skin, and is also widely used in a variety of skin-care products. In this study, we investigated the modulatory roles of collagen I on human immortalized keratinocytes HaCaT, especially when cells were irradiated with UVB. Interestingly, the cells grown on plates coated by molecular collagen I, but not fibrillar collagen I, acquired certain resistance against UVB damages, as shown by increased survival and reduced apoptosis.

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Objectives: To evaluate the long-term outcomes of patients treated under our perfusion-based strategy and assess whether conservative treatment without surgical treatment under our strategy is acceptable.

Materials And Methods: A total of 315 adult and 137 pediatric MMD patients (follow-up period ≥ 3 years from 2001 to 2020) were included. Follow-up events in each patient group (pediatric or adult, surgically treated or conservatively treated) were evaluated and compared to each other using a log-rank test.

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Article Synopsis
  • * Silibinin, a compound from Silybum marianum, shows promise as a treatment for sporadic AD, improving cell survival and reducing cognitive impairments in tested models.
  • * The study reveals that silibinin protects against ferroptotic damage by modulating proteins like p53 and GPX4, and also inhibits the inflammatory response triggered by oxidized DNA leakage, indicating a potential therapeutic pathway for AD.
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Type 2 diabetes (T2DM) is induced by the abundance of glucose and lipids, which causes glucolipotoxicity to the pancreatic β-cells. Silibinin is a natural flavonoid possessing the regulatory activity on insulin production and therapeutic activity in diabetic mice; however, its effect on glucolipotoxicity is not fully explained. This in vitro study investigates the effects of silibinin on palmitic acid (PA) and high glucose (HG)-induced cell loss and ferroptosis of rat insulinoma INS-1 cells.

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Ultraviolet B (UVB) irradiation causes skin inflammation and apoptosis. Mitochondria are highly dynamic and undergo constant fusion and fission that are essential for maintaining physiological functions of cells. Although dysfunction of mitochondria has been implicated in skin damages, little is known about the roles of mitochondrial dynamics in these processes.

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Ultraviolet B (UVB) irradiation causes skin damages. In this study, we focus on the involvement of mitochondrial disorders in UVB injury. Surprisingly, UVB irradiation increases the amounts of mitochondria in human immortalized keratinocytes HaCaT.

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Background: Over-activation of N-methyl-D-aspartate receptors (NMDARs) is involved in sporadic Alzheimer's disease. Silibinin, a natural flavonoid gained from the seeds of Silybum marianum, exerts neuroprotective effects on sporadic AD models, but its impacts on NMDARs remain unknown.

Purpose: To study silibinin's regulatory effects on NMDARs pathway in sporadic AD models.

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The recommended treatment strategy for stage 3 bisphosphonate-related osteonecrosis of the jaw (BRONJ) is currently rigid plate fixation without bone reconstruction. However, a recent systematic review indicated the utility of microsurgical reconstruction after resection of BRONJ. Several types of flaps have been described but their applications are controversial.

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Excessive physical exercise (overtraining, OT) charactered by long-term and excessive training results in the damage of multiple vital tissues including hippocampus which plays a critical role in learning and memory. A combination of dasatinib (D) plus quercetin (Q) (D+Q) belongs to senolytic drugs which selectively kill senescent cells in vitro and vivo. In this study, the rats that suffered a five-week excessive swimming training were subjected to the oral administration of D+Q.

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Purpose: Severe lymphedema is difficult to treat because of the associated extensive scar formation. Therefore, preventing scar formation might alleviate the severity of lymphedema following lymphadenectomy. In this study, we evaluated the usefulness of flap transfer, performed immediately after lymphadenectomy, for preventing scar formation.

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Alcoholic liver disease has become one of the main causes of liver injury, and its prevention and cure are important medical tasks. Silibinin, a natural flavonoid glycoside, is a conventional hepatic protectant. This study elucidates the modulation of ferroptosis in silibinin's protective effects on ethanol- or acetaldehyde-induced liver cell damage by using human carcinomatous liver HepG2 cells and immortalized liver HL7702 cells.

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The extracellular matrix (ECM) regulates cell behavior through signal transduction and provides a suitable place for cell survival. As one of the major components of the extracellular matrix, type I collagen is involved in regulating cell migration, proliferation and differentiation. We present a system in which 3T3-L1 preadipocyte cells are induced for adipogenic differentiation on type I collagen coated dishes.

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Article Synopsis
  • Japanese encephalitis virus (JEV) is primarily transmitted in the Asian-Pacific region through Culex tritaeniorhynchus mosquitoes and affects humans severely.
  • Researchers discovered JEV in unfed Haemaphysalis flava ticks collected in Japan, even during months when the main mosquito vector was not active.
  • The findings include detection of JEV RNA in ticks and some evidence of viral replication, but there’s still uncertainty whether ticks serve as a reservoir for the virus since no infectious JEV was isolated.
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Introduction: Rectourethral fistula (RUF) after prostatectomy is a rare complication; however, when it occurs it is likely to be intractable and treatment requires surgical closure of the fistula. Several approaches to fistula closure have been reported, but there is no established treatment.

Case Presentation: The patient was a 66-year-old man who had undergone robot-assisted laparoscopic radical prostatectomy for prostate cancer.

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Silibinin is a natural polyphenolic flavonoid, isolated from the seeds of the milk thistle of Silybum marianum (L.) Gaertn. Silibinin has been widely used clinically as a traditional medicine for liver diseases.

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