Publications by authors named "Hatsumi Monjusho"
The use of bovine brain has been prohibited in many countries because of the world-wide prevalence of mad cow disease, and thus porcine brain is expected to be a new source for the preparation of gangliosides. Here, we report the presence of a ganglioside in porcine brain which is strongly resistant to hydrolysis by endoglycoceramidase, an enzyme capable of cleaving the glycosidic linkage between oligosaccharides and ceramides of various glycosphingolipids. Five major gangliosides (designated PBG-1, 2, 3, 4, 5) were extracted from porcine brain by Folch's partition, followed by mild alkaline hydrolysis and PBA column chromatography.
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- The nucleotide sequence of ceramidase from Dictyostelium discoideum is available in the DDBJ, with this enzyme showing unique characteristics.
- Despite having characteristics similar to neutral ceramidases, the enzyme exhibits its highest activity at an acidic pH of around 3.0.
- When the gene was knocked out in the slime mold, CDase activity at acidic pH was eliminated, indicating the enzyme's pH dependency is intrinsic rather than due to post-translational modifications specific to the slime mold.