Publications by authors named "Hatamoto Masashi"

A novel, gelatinous, colony-forming, rod-shaped bacterial strain, designated IK01 was isolated from biofilms formed on the membrane surface of a sewage-treating membrane bioreactor (MBR). Strain IK01 produced gelatinous and almost transparent colonies at lower medium concentrations. Fourier transform infrared analysis of the gelatinous colony matrix showed that the matrix could be a biofilm substance.

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Providing anaerobic environments can enhance the denitrification capacity of wastewater treatment systems. This study developed an aerobic-anaerobic downflow hanging sponge (DHS) reactor to increase denitrification. A siphon tube was integrated with a conventional DHS to create anaerobic conditions by controlling a water column inside the reactor.

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This study presents a novel approach to sustainable aquaculture by integrating biofloc technology (BFT) with a compact down-flow hanging sponge (DHS) reactor. The integrated BFT-DHS system effectively removed nitrogen compounds while maintaining ammonia-nitrogen (NH-N) concentrations below 1 mg-N L without water exchange. Application of this system in a tank bred with juvenile Oreochromis niloticus showed a high NH-N removal rate of up to 97 % and nitrite (NO -N) concentrations were maintained at 0.

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Aquaponics is a promising solution for addressing food security concerns. Nonetheless, an effective water-purification system is necessary to achieve high and stable yields of fish and vegetables. This study aimed to evaluate the nitrification and oxygen transfer performance of a laboratory-scale down-flow hanging sponge (DHS) reactor with aquaponics system to treat water in an closed-aquaculture system.

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In a membrane bioreactor (MBR) system, in situ sludge reduction techniques induce membrane fouling. To address this challenge, we incorporated a rotating mesh carrier, which can adsorb organic matter and provide a habitat for metazoans, into the anoxic tank of a conventional anoxic/oxic-MBR (A/O-MBR) system, termed rotating biological contactor-MBR (RBC-MBR), and evaluated treatment performance. Over 151 days, lab-scale RBC-MBR and A/O-MBR were used to treat municipal sewage.

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In recirculating aquaculture systems (RAS), maintaining water quality in aquaculture tanks is a paramount factor for effective fish production. A down-flow hanging sponge (DHS) reactor, a trickling filter system used for water treatment of RAS that employs sponges to retain biomass, has high nitrification activity. However, nitrification in seawater RAS requires a long start-up time owing to the high salinity stress.

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Article Synopsis
  • The study examined the effectiveness of a down-flow hanging sponge (DHS) reactor integrated with a sedimentation tank for wastewater treatment over 500 days with various hydraulic retention times (HRT).
  • Despite the shortest HRT of 0.3 hours leading to nitrogen concentrations that affected nitrification, the effluent's BOD remained stable at around 4.0 mg·L, and all discharge standards were met during the experiment.
  • The research found that the population of comammox bacteria in the sponge biomass (5.58 to 13.2 × 10 copies·mL) suggested that these sponges create a suitable environment for their growth, potentially enhancing nitrification in the process.
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A novel bio-carrier, PVA/Fe/starch gel bead, was fabricated and developed to enhance the anaerobic treatment performance of synthetic azo dye-containing wastewater. PVA-gel beads with 5 % magnetite and 0.5 % starch were optimal for physical strength and treatment performance.

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Two myxobacterial strains (KH5-1 and NO1) were isolated from the activated sludge tanks treating municipal sewage wastewater in Japan. These strains were recognised as myxobacteria based on their phenotypic characteristics of swarming colonies and fruiting bodies. Phylogenetic analyses using the 16S rRNA gene revealed that strains KH5-1 and NO1 were affiliated with the genus Corallococcus, with the closest neighbours being Corallococcus exercitus AB043A (99.

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Highly colored azo dye-contaminated wastewater poses significant environmental threats and requires effective treatment before discharge. The anaerobic azo dye treatment method is a cost-effective and environmentally friendly solution, while its time-consuming and inefficient processes present substantial challenges for industrial scaling. Thus, the use of iron materials presents a promising alternative.

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In this study, we aimed to establish high-rate biological treatment of purified terephthalic acid (PTA) and dimethyl terephthalate (DMT) wastewater that minimizes the inhibitory effects of high concentration benzoate and acetate. To achieve this, we developed a novel bioreactor system and biostimulation strategy. An internal two-stage upflow anaerobic (ITUA) reactor was operated with (i) a packed bed containing green tuff medium underlying (ii) a compartment seeded with anaerobic granular sludge.

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Myxobacteria are known as prolific producers of secondary metabolites with a unique and wide spectrum of bioactivities. Here, we report draft genome sequences of KH5-1 and NO1, myxobacteria isolated from activated sludge, which consist of 9.89 and 9.

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The cycle of life and death and Earth's carbon cycle(s) are intimately linked, yet how bacterial cells, one of the largest pools of biomass on Earth, are recycled back into the carbon cycle remains enigmatic. In particular, no bacteria capable of scavenging dead cells in oxygen-depleted environments have been reported thus far. In this study, we discover the first anaerobes that scavenge dead cells and the two isolated strains use distinct strategies.

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Article Synopsis
  • Understanding microbial interactions is crucial for enhancing wastewater treatment efficiency, particularly regarding predation and parasitism, which can help reduce excess sludge.
  • A study analyzed 600 activated sludge samples from various wastewater treatment plants and identified 106 shared microbial families that correlate with total carbon and nitrogen levels, indicating their potential role in wastewater remediation.
  • Specific bacterial groups, like Bdellovibrionota, Myxococcota, and Patescibacteria, not only showed a consistent presence across the samples but also displayed genes linked to predatory behavior, suggesting they contribute to the breakdown of organic materials in wastewater.
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This study evaluated the effects of acetone on the anaerobic degradation of synthetic latex wastewater, which was simulated from the wastewater of the deproteinized natural rubber production process, including latex, acetate, propionate, and acetone as the main carbon sources, at a batch scale in 5 cycles of a total of 60 days. FeO was applied to accelerate the treatment performance from cycle 3. Acetone was added in concentration ranges of 0%, 0.

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Microbial biofilm development on the membrane surface of bioreactors results in membrane flux decline (biofouling). Biofouling is one of the most severe problems limiting the use of these bioreactors. For detailed understanding of the biofouling, microbial community and dissolved organic matter analyses have been performed in recent decades.

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This study aimed to evaluate the nitrogen removal of a post-treatment system for natural rubber processing wastewater (NRPW) under low chemical oxygen demand to total nitrogen (COD/TN) ratios without any supplemental external carbon source. The system including a downflow hanging sponge (DHS) reactor and an upflow anaerobic reactor (UAR) was operated in two phases. In phase 1 (day 0-102), under a nitrogen loading rate (NLR) of 0.

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The main objective of this study was to investigate the cause of gel fouling in membrane bioreactors (MBRs) treating real sewage in terms of soluble microbial products (SMPs) and microbial aspects. Two anoxic/oxic-MBRs were operated as the control reactor (S1) and the sludge loading rate increased reactor (S2). The reactors were operated under low-temperature around 11 °C conditions.

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Conventional activated sludge (AS) systems are widely used to treat domestic sewage worldwide. However, the removal of nitrogen in the AS system is limited, and its concentration in the effluent exceeds the recommended values in the discharge standards. In this study, a pilot experiment was conducted to improve nitrogen removal during municipal sewage treatment by operating AS and anaerobic-aerobic (AO) systems under low dissolved oxygen (DO) conditions of less than 0.

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Aerobic ammonia and nitrite oxidation reactions are fundamental biogeochemical reactions contributing to the global nitrogen cycle. Although aerobic nitrite oxidation yields 4.8-folds less Gibbs free energy (∆G ) than aerobic ammonia oxidation in the NH -feeding marine recirculating trickling biofilter reactors operated in the present study, nitrite-oxidizing and not ammonia-oxidizing Nitrospira (sublineage IV) outnumbered ammonia-oxidizing Nitrosomonas (relative abundance; 53.

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An anaerobic baffled reactor (ABR) is one of the useful wastewater treatment technologies, but the knowledge about its treatment performance for actual wastewater with load fluctuation is limited. The organic removal performance of an ABR for treating supermarket wastewater was evaluated. The ABR, which consisted of eight columns, was examined under four hydraulic retention time (HRT) conditions of 19.

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Potato ( L.) is a major global food crop. Contemporary potato production largely utilizes micropropagation to produce healthy seed potatoes.

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This study was conducted to assess the potential of a sponges-submerged anaerobic baffled reactor (SS-ABR) for enhancing the processing performance of azo dye-contaminated wastewater. A lab-scale four-compartment SS-ABR, with a total volume of 10 L, was operated at 30 °C for 180 days. A total of 14 polyurethane sponges were added in each compartment to treat synthetic wastewater including a commercial azo dye Hellozol HSR Reactive Black.

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Tofu is widely processed in East and Southeast Asian countries. During the production, highly polluted wastewater is discharged. This wastewater is commonly treated using a high-rate anaerobic wastewater treatment process; however, several organic compounds and nitrogen remain in the anaerobic effluent.

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Down-flow hanging sponge (DHS) reactor that is sponge-based trickling filter was considered to be an alternative aerobic treatment system for low strength sewage treatment under tropical conditions. This study aims to determine the process performance of the DHS reactor combined with a pre-treatment sedimentation tank (SED) system installed at the municipality sewage treatment plant in Khon Kaen, Thailand over, 1,600 days. The DHS reactor was operated with three operational periods: low (0.

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