Color-neutral and reversible tissue transparency enables longitudinal deep-tissue imaging in live mice.

Unlabelled: Light scattering in biological tissue presents a significant challenge for deep imaging. Our previous work demonstrated the ability to achieve optical transparency in live mice using intensely absorbing dye molecules, which created transparency in the red spectrum while blocking shorter-wavelength photons. In this paper, we extend this capability to achieve optical transparency across the entire visible spectrum by employing molecules with strong absorption in the ultraviolet spectrum and sharp absorption edges that rapidly decline upon entering the visible spectrum.

Spontaneous enteric nervous system activity precedes maturation of gastrointestinal motility.

Spontaneous neuronal network activity is essential in development of central and peripheral circuits, yet whether this is a feature of enteric nervous system development has yet to be established. Using gastrointestinal (GI) motility assays with unbiased computational analyses, we identify a previously unknown pattern of spontaneous neurogenic GI motility. We further show that this motility is driven by cholinergic signaling, which may inform GI pharmacology for preterm patients.

Modeling the Dynamics of a Secondary Neurodegenerative Injury Following a Mild Traumatic Brain Injury.

During a traumatic brain injury (TBI), there is an injection of glial fibrillary acidic protein (GFAP) from the brain into the bloodstream through a lesion in the blood-brain barrier (BBB). In the blood, a bio controller responds by up-regulating Immunoglobulin G (IgG) production into the bloodstream to remove the excess protein. Here, we model the concentrations over time of GFAP and IgG in the bloodstream following a mild TBI.