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Introduction: Public primary healthcare facilities, the cornerstone of the Tunisian health system, were impacted by the Covid 19 crisis as all health systems in the world.

Aim: This study aims to assess this impact of the pandemic.

Methods: We analyzed the budgetary evolution of the basic healthcare group (BHG) of Medenine and Djerba between 2019 and 2020.

Comparison of Mineral Contents in Three Different Tobacco Formulations.

We identified and quantified a variety of mineral elements in 18 tobacco samples purchased from a Tunisian market. In total, 25 mineral elements have been measured in cigarettes, water pipe tobacco, and smokeless tobacco using inductively coupled plasma-optical emission spectroscopy following microwave-assisted digestion. Statistical analyses were performed using SPSSTM, version 18.

LC-ESI-MS quali-quantitative determination of phenolic constituents in different parts of wild and cultivated Astragalus gombiformis.

High-performance liquid chromatography electrospray ionization mass spectrometry (LC-ESI-MS) profiling of the MeOH extract of Astragalus gombiformis Pomel (Fabaceae) aerial parts guided the isolation of seven phenolic compounds among which 7-methylquercetin 3-O-α-l-rhamnopyranosyl-(1→2)-β-D-galactopyranoside (2) and 7-methylquercetin 3-O-α-L-rhamnopyranosyl-(1→2)-[6-O-(3-hydroxy-3-methylglutaryl)-β-D-galactopyranoside] (7) whose structures were elucidated by NMR and ESI-MS experiments. The radical scavenging activities of isolated compounds were investigated, by using the TEAC assay. Furthermore quantitative analyses were performed by LC-ESI-MS and applied to the comparative profiling of different parts (aerial parts, leaves and stems) of cultivated and wild A.

Biological and chemical study of Astragalus gombiformis.

Extracts of aerial parts and roots of wild Astragalus gombiformis Pomel were tested for their antibacterial, antioxidant, and insecticidal activities and contents of phenolic compounds. Antibacterial activity was tested by the paper disk agar diffusion method and determination of the minimal inhibitor concentration. Among the tested extracts, three extracts (methanol, chloroform, and ethyl acetate) from aerial parts and two extracts (water, methanol) from roots exhibited diameters of inhibition zone equal or above 12 mm (at 150 microg/ disk) and minimal inhibitor concentrations ranging between 233 and 1250 microg/ml.

Development of a method of clozapine dosage by selective electrode to the iodides.

Clozapine (Leponex(®)), the main neuroleptic indicated in the treatment of resistant schizophrenia, requires therapeutic monitoring because of its side effects and the individual variability in metabolism. In addition, several cases of intoxication by this drug were described in the literature. In this work, we studied the indirect dosage of clozapine by selective electrode to the iodides for the optimization of an analytical protocol allowing therapeutic monitoring and the diagnosis of intoxication and/or overdose.

Variation in volatiles of Astragalus gombiformis Pomel.

The essential oils obtained by hydrodistillation (Clevenger apparatus) from aerial parts of Astragalus gombiformis were analysed by gas chromatography coupled with mass spectrometry (GC/MS). This study showed that the A. gombiformis essential oils are complex mixtures of important natural compounds, which varied qualitatively and quantitatively between cultivated and wild plants and between phenological stages of development.