Assessment of prevalence, antibiotic resistance, and virulence profiles of biofilm-forming isolated from raw seafood in Bangladesh.

are often resistant to different classes of antibiotics, harbor virulence determinants, and produce biofilm. The presence of in raw seafood exhibits serious public health significance. This study aimed to identify antibiotic resistance patterns and virulence factors in biofilm-forming strains extracted from seafood in Bangladesh.

Detection of prolong excretion of in stool specimens of a 7-year-old child by a newly developed gene-based quantitative real-time PCR method and molecular characterization of the isolates.

is an emerging zoonotic foodborne pathogen The clinical significance of this bacterium has increasingly been recognized worldwide. However, diagnostic method has not yet been established and its clinical manifestations are not fully understood. Here, we show that an gene-based quantitative real-time PCR (qRT-PCR) developed in this study is 100% specific and sensitive when tested with 39  and 36 non- strains, respectively.

Epidemiology and molecular characterization of Feline panleukopenia virus from suspected domestic cats in selected Bangladesh regions.

Feline panleukopenia (FPL) is a highly contagious cat disease and is endemic in Bangladesh. The study aims to describe the epidemiology and molecular characterization of the Feline panleukopenia virus from the suspected domestic cats in selected Bangladesh regions. Randomly, 161 rectal swabs were collected from the pet hospitals between July 2021 and December 2022.

Resistance Profiles and Virulence Determinants in Biofilm-Forming Isolated from Raw Seafood in Bangladesh.

Pathogenic, antibiotic-resistant, and biofilm-forming bacteria can be transferred to humans through the consumption of contaminated seafood. The present study was carried out to determine antibiotic resistance profiles and virulence determinants in biofilm-forming isolated from seafood in Bangladesh. A total of 150 seafood samples, including shrimp (n = 50), crabs (n = 25), and marine fish (n = 75), were screened using cultural, staining, biochemical, polymerase chain reaction (PCR), Congo red (CR), and disk diffusion (DD) assays.

Draft genome sequence of BAU_KME002 strain isolated from egg surface in Bangladesh.

This report describes the genome sequence of the BAU_KME002 strain isolated in Bangladesh in 2021 from a chicken egg surface. Our assembled genome had 50 contigs, an estimated genome length of 2,866,882 bp (with coverage of 90.0×), 36 predicted antibiotic resistance genes, and 28 predicted virulence factor genes.

Draft genome sequence of biofilm-forming methicillin-resistant MTR_V1 strain isolated from a ready-to-eat food in Bangladesh.

This announcement provides the genome sequence of the biofilm-forming methicillin-resistant MTR_V1 strain isolated from a ready-to-eat food sample in Bangladesh. Our assembled genome had a length of 2.8 Mb, 27 contigs, two CRISPR arrays, 38 predicted antibiotic resistance genes, and 66 predicted virulence factor genes.

Draft genome sequence of biofilm-forming BAU_Ef01 strain isolated from shrimp () in Bangladesh.

Here, we sequence and analyze a biofilm-forming strain of BAU_Ef01 isolated from a shrimp in Bangladesh. The whole genome of the strain had a length of 2,862,301 bp, 38 contigs, an average G+C content of 37.36%, 80.

Diversity of spp. and genomic characteristics of isolated from clinical mastitis of cattle in Bangladesh.

Introduction: Streptococci are the major etiology in mastitis in dairy cattle, a cause of huge economic losses in the dairy industries. This study was aimed to determine the diversity of spp. isolated from clinical mastitis of cattle reared in Bangladesh.

Antimicrobial Resistance Profiles, Virulence Determinants, and Biofilm Formation in Enterococci Isolated from Rhesus Macaques (): A Potential Threat for Wildlife in Bangladesh?

Enterococci are commensal bacteria that inhabit the digestive tracts of animals and humans. The transmission of antibiotic-resistant genes through human-animal contact poses a potential public health risk worldwide, as zoonoses from wildlife reservoirs can occur on every continent. The purpose of this study was to detect spp.

Extended-spectrum beta-lactamase in isolated from humans, animals, and environments in Bangladesh: A One Health perspective systematic review and meta-analysis.

Extended-spectrum beta-lactamase (ESBL)-producing has been linked to both life-threatening hospital- and community-acquired infections across the globe. Here, we conducted a systematic review and meta-analysis to evaluate the prevalence of ESBL in isolated from humans, animals, and environments in Bangladesh. Following the preferred reporting items for systematic reviews and meta-analyses (PRISMA) guidelines, the current systematic review and meta-analysis was taken into account for studies published between 2010 and 2021 in peer-reviewed journals.

Presence of Functionally Active Cytolethal Distending Toxin Genes on a Conjugative Plasmid in a Clinical Isolate of Providencia rustigianii.

Providencia rustigianii is potentially enteropathogenic in humans. Recently, we identified a strain carrying a part of the gene homologous to that of that produces an exotoxin called cytolethal distending toxin (CDT), encoded by three subunit genes (, , and ). In this study, we analyzed the strain for possible presence of the entire gene cluster and its organization, location, and mobility, as well as expression of the toxin as a putative virulence factor of .

Deep learning-based bacterial genus identification.

Objectives: This study aimed to develop a computerized deep learning (DL) technique to identify bacterial genera more precisely in minimum time than the usual, traditional, and commonly used techniques like cultural, staining, and morphological characteristics.

Materials And Methods: A convolutional neural network as a part of machine learning (ML) for bacterial genera identification methods was developed using python programming language and the Keras API with TensorFlow ML or DL framework to discriminate bacterial genera, e.g.

First report on the molecular characteristics of mcr-1 colistin resistant E. coli isolated from retail broiler meat in Bangladesh.

Poultry meat is considered as a potential source of colistin resistant Escherichia coli (CREC). This study aimed to determine the prevalence and characteristics of CREC in broiler meat and ascertain their possible zoonotic potential(s). Broiler meat (n = 104) comprising 26 of each of the thigh, breast, liver, and proventriculus-gizzard was purchased from the retail outlets, Bangladesh.

Antimicrobial Resistance, Virulence Profiles, and Public Health Significance of Isolated from Clinical Mastitis of Cattle in Bangladesh.

This study was designed to identify from clinical mastitis of cattle and determine their antimicrobial resistance and virulence determinants to evaluate their potential public health significance. A total of 105 composite milk samples (80 from cattle with clinical mastitis and 25 from apparently healthy cattle) were analyzed. were isolated by culturing on enterococcal selective media and identified by PCR and sequencing.

A Cross-Sectional Seroepidemiological Study on Infectious Bursal Disease in Backyard Chickens in the Mymensingh District of Bangladesh.

Infectious bursal disease (IBD) is a highly contagious disease that causes significant economic loss in chickens. A cross-sectional study was conducted in the Mymensingh district of Bangladesh to determine the seroprevalence of IBD virus (IBDV) antibodies in backyard chickens and their association with different epidemiological risk factors. A total of 460 serum samples were randomly collected from backyard chickens that had not been previously vaccinated against IBDV.

Review of anthrax: A disease of farm animals.

Anthrax is a rapidly fatal infectious disease affecting herbivores and people. In the farm animals, cattle and sheep are more susceptible, followed by goats and horses, while dwarf pigs and Algerian sheep are relatively resistant. , the causative agent of anthrax, produces spores and persists for decades in the soil, initiating an outbreak through a favorable climate shift.

Hierarchical true prevalence, risk factors and clinical symptoms of tuberculosis among suspects in Bangladesh.

Background: The study was aimed to estimate the true prevalence of human tuberculosis (TB); identify risk factors and clinical symptoms of TB; and detect rifampicin (RIF) sensitivity in three study areas of Bangladesh.

Methods: The cross-sectional study was conducted in three Bangladesh districts during 2018. Potential risk factors, clinical symptoms, and comorbidities were collected from 684 TB suspects.

Risk factors for bovine rotavirus infection and genotyping of bovine rotavirus in diarrheic calves in Bangladesh.

Bovine rotavirus (BRV) is considered the leading cause of calf diarrhea worldwide, including Bangladesh. In this study we aimed to identify risk factors for BRV infection and determine the G and P genotypes of BRV strains in diarrheic calves. Fecal samples were collected from 200 diarrheic calves in three districts between January 2014 and October 2015.

Virulence determinants and antimicrobial resistance of isolated from bovine clinical mastitis in some selected dairy farms of Bangladesh.

is one of the major significant pathogens causing mastitis, the most complex and costly diseases in the dairy industry worldwide. Present study was undertaken to isolate, detect the virulence factors, phylogroup, antimicrobial susceptibility and antimicrobial resistance genes in from cows with clinical mastitis. A total of 68 milk samples comprising 53 from clinical mastitis and 15 from apparently healthy cattle were collected from four different established dairy farms in Bangladesh.

Detection, Isolation, and Molecular Characterization of Escherichia albertii from Wild Birds in West Japan.

Escherichia albertii is an emerging zoonotic foodborne pathogen. Several outbreaks of E. albertii have occurred, particularly in Japan.