Publications by authors named "Hassan Elbery"
This work was conducted to employ a whole cell-based biosensor to monitor toxicity of heavy metals in water and wastewater. An isolate of industrial wastewater bacterium, Acinetobacter sp. DF4, was genetically modified with lux reporter gene to create a novel bioluminescent bacterial strain, designated as DF4/PUTK2.
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- A bioluminescent strain of Acinetobacter (DF4/PUTK2) was used to evaluate the toxicity of phenolic compounds and determine the effective concentration that causes 50% inhibition (EC50) in just 5 minutes.
- The assay results from DF4/PUTK2 were compared to the Vibrio fischeri luminescence inhibition assay for validation.
- The study identified Ca-alginate as the best immobilization material for the bioluminescent cells, allowing them to maintain their toxicity detection capabilities for at least 8 weeks in storage, making it suitable for ecotoxicity testing of phenol-contaminated samples.