Hydrogeochemical Evaluation of Groundwater Quality Parameters for Ogallala Aquifer in the Southern High Plains Region, USA.

The purposes of this study are to analyze the groundwater quality of the Ogallala Aquifer and evaluate the hydrological characteristics in the southern High Plains region of the Permian Basin, Texas. Levels of chloride, fluoride, nitrate, selenium, pH, and total dissolved solids (TDS) were analyzed for the period 1990-2016. Data concerning a total of 133 wells were collected from the Texas Water Development Board (TWDB), which is an open database provided by the US government.

KH domain containing RNA-binding proteins coordinate with microRNAs to regulate Caenorhabditis elegans development.

MicroRNAs (miRNAs) and RNA-binding proteins (RBPs) regulate gene expression at the post-transcriptional level, but the extent to which these key regulators of gene expression coordinate their activities and the precise mechanisms of this coordination are not well understood. RBPs often have recognizable RNA binding domains that correlate with specific protein function. Recently, several RBPs containing K homology (KH) RNA binding domains were shown to work with miRNAs to regulate gene expression, raising the possibility that KH domains may be important for coordinating with miRNA pathways in gene expression regulation.

Computational Studies of Relative Stabilities of Low-Spin d cis- and trans-[M(en)X] Complexes (M = Co, Rh, Ir): Steric and Electronic Effects in the Context of the Structural Trans Influence.

Computational studies of low spin d cis- and trans-[M(en)X] complexes (M = Co, Rh, Ir) employing multiple model chemistries find that isomer preferences fall into three categories. Complexes where X is largely a σ-donor (H, CH, CF) prefer cis geometries, in keeping with predictions associated with the trans influence series. Complexes where this donor characteristic is augmented by π acceptor behavior (B(CF), BCl, SiCl) evince even greater preference for cis geometries.

Re-Envisioning Tarascan Temporalities and Landscapes: Historical Being, Archaeological Representation, and Futurity in Past Social Processes.

We apply a phenomenological perspective on landscape and geographic information system (GIS) applications in order to theorize how human perception and agency were likely implicated in processes of the formation of the late pre-Hispanic Tarascan State of West Central Mexico. The relatedness of landscape features in space or place-based perception has been well theorized; here, we further consider the relationality of places through time. In the changing landscape of the Lake Pátzcuaro Basin, the demographic and political core of the Tarascan State, temporality must have been vitally important to inhabitants of the basin.

Factors Influencing Church Choice: An Exploration of Responses from New Attendees at Growing Canadian Mainline Churches.

Seventy new attendees of growing Mainline Protestant churches in Canada agreed to be interviewed regarding what attracted them to, and kept them at, their current congregation. A variety of responses revealed that, in part, the new attendees were drawn to their Mainline Protestant church by aspects of Conservative Protestant theology. Contemporary theories of group membership are used to explore the links between Conservative Protestant theology, group cohesion, and, to a lesser extent, church growth.

Simulated birdwatchers' playback affects the behavior of two tropical birds.

Although recreational birdwatchers may benefit conservation by generating interest in birds, they may also have negative effects. One such potentially negative impact is the widespread use of recorded vocalizations, or "playback," to attract birds of interest, including range-restricted and threatened species. Although playback has been widely used to test hypotheses about the evolution of behavior, no peer-reviewed study has examined the impacts of playback in a birdwatching context on avian behavior.

Darwin's manufactory hypothesis is confirmed and predicts the extinction risk of extant birds.

In the Origin of Species Darwin hypothesized that the "manufactory" of species operates at different rates in different lineages and that the richness of taxonomic units is autocorrelated across levels of the taxonomic hierarchy. We confirm the manufactory hypothesis using a database of all the world's extant avian subspecies, species and genera. The hypothesis is confirmed both in correlations across all genera and in paired comparisons controlling for phylogeny.

Depauperate avifauna in plantations compared to forests and exurban areas.

Native forests are shrinking worldwide, causing a loss of biological diversity. Our ability to prioritize forest conservation actions is hampered by a lack of information about the relative impacts of different types of forest loss on biodiversity. In particular, we lack rigorous comparisons of the effects of clearing forests for tree plantations and for human settlements, two leading causes of deforestation worldwide.

Exploring the temperature-stress metabolome of Arabidopsis.

Metabolic profiling analyses were performed to determine metabolite temporal dynamics associated with the induction of acquired thermotolerance in response to heat shock and acquired freezing tolerance in response to cold shock. Low-M(r) polar metabolite analyses were performed using gas chromatography-mass spectrometry. Eighty-one identified metabolites and 416 unidentified mass spectral tags, characterized by retention time indices and specific mass fragments, were monitored.

Diurnal regulation of Hsp70s in leaf tissue.

Steady-state mRNA levels for three Hsp70s were found to be regulated by a distinctive light/dark mechanism in spinach leaves. Messenger RNAs for the chloroplast stromal and two cytosolic forms displayed a diurnal expression pattern under isothermal conditions that appeared to be independent of circadian control. While protein blot data showed relatively constant Hsp70 protein levels, the higher Hsp70 mRNA levels in the light paralleled the diurnal cycle of total cell protein synthesis.

Coordinate and non-coordinate expression of the stress 70 family and other molecular chaperones at high and low temperature in spinach and tomato.

Stress 70 molecular chaperones are found in all the major subcellular compartments of plant cells, and they are encoded by a multigene family. Twelve members of this family have been identified in spinach. The expression of the stress 70 molecular chaperones in response to heat shock is well-known and it appears that low temperature exposure can also stimulate their expression.

The effect of predation on begging-call evolution in nestling wood warblers.

I combined a comparative study of begging in ground- and tree-nesting wood warblers (Parulidae) with experimental measures of the predation costs of warbler begging calls. Throughout their development, ground-nesting warbler nestlings had significantly higher-frequency begging calls than did tree-nesting warblers. There was also a trend for ground-nesting birds to have less rapidly modulated calls.

Calling all patients.

Hospitals must establish patients' expectations of care and their satisfaction with the care delivered. This telephone survey tool measures patient satisfaction based on empathy, communication, competence and continuity of care, and amenities.

Characterization of a gene for spinach CAP160 and expression of two spinach cold-acclimation proteins in tobacco.

The cDNA sequence for CAP160, an acidic protein previously linked with cold acclimation in spinach (Spinacia oleracea L.), was characterized and found to encode a novel acidic protein of 780 amino acids having very limited homology to a pair of Arabidopsis thaliana stress-regulated proteins, rd29A and rd29B. The lack of similarity in the structural organization of the spinach and Arabidopsis genes highlights the absence of a high degree of conservation of this cold-stress gene across taxonomic boundaries.

A study of caloric restriction and cardiovascular aging in cynomolgus monkeys (Macaca fascicularis): a potential model for aging research.

Caloric restriction has been demonstrated to retard aging processes and extend maximal life span in rodents, and is currently being evaluated in several nonhuman primate trials. We initiated a study in 32 adult cynomolgus monkeys to evaluate the effect of caloric restriction on parameters contributing to atherosclerosis extent. Following pretrial determinations, at which time a baseline measure of ad libitum (ad lib) dietary intake was assessed, animals were randomized to an ad lib fed group (control) or a caloric restriction group (30% reduction from baseline intake).

Differential influence of ATP on native spinach 70-kilodalton heat-shock cognates.

A constitutively expressed class of 70-kD heat-shock cognate (HSC70) proteins from spinach leaf tissue was purified based on their affinity for ATP-agarose. The affinity-purified spinach proteins were resolved into at least three different forms on two-dimensional gels. Under native conditions, and iN the absence of ATP, the affinity-purified proteins were separated into three molecular mass classes by gel-filtration chromatography; a monomer of 85 kD, a multimer of 280 kD, and a large molecular mass oligomer of > 650 kD.

Structural organization of the spinach endoplasmic reticulum-luminal 70-kilodalton heat-shock cognate gene and expression of 70-kilodalton heat-shock genes during cold acclimation.

The 70-kD heat-shock proteins (HSP70s) are encoded by a multigene family in eukaryotes. In plants, the 70-kD heat-shock cognate (HSC70) proteins are located in organellar and cytosolic compartments of cells in most tissues. Previous work has indicated that HSC70 proteins of spinach (Spinacia oleracea) are actively synthesized during cold-acclimating conditions.

Antigen binding of a mouse monoclonal IgG1 is inactivated by heating but not by freeze/thaw cycling.

A mouse monoclonal antibody in ascitic fluid or in purified form was subjected to heating or repeated freeze/thaw cycles. Heating the antibody at 80 degrees C for 10 min completely destroyed its ability to bind to antigen. Unpurified antibody in ascitic fluid or purified antibody in buffer was frozen to -196 degrees C in liquid nitrogen and then thawed at room temperature.

Characterization of a spinach gene responsive to low temperature and water stress.

The characterization of a cDNA for an 85 kDa spinach protein, CAP85 (cold acclimation protein) that is responsive to cold acclimation and water stress is described. Both transcript and protein levels are increased during cold acclimation and water stress. A novel characteristic of CAP85 is the presence of an 11 amino acid, lysine-rich repeat, common to Group 2 LEAs (late embryogenesis abundant proteins), which is included within a larger repeating motif present in 11 copies.