Measurement of low molecular weight urinary monoamines by gas chromatography.

A simple gas chromatographic method for the measurement of low molecular weight urinary monoamines has been developed without use of a prior isolation procedure. The primary and secondary monamines in urine were directly converted into their 2,4-dinitrophenyl derivatives with 2,4-dinitrobenzensulfonate in aqueous alkaline solution, which after extraction with benzene and concentration, were submitted to gas chromatography. The application of this method to the determination of methylamine and dimethylamine in urine from normal subjects and hepatic patients is described.

Congenital left atrial enlargement. A case report with special reference to myocardial fine structure.

A case of intrapericardial congenital left atrial enlargement was reported. The patient is an asymptomatic 3 year-old girl with abnormal cardiac silhouette seen on a chest roentgenogram. Angiography showed marked left atrial enlargement, and the diagnosis of intrapericardial congenital left atrial enlargement was confirmed on operation.

Progestational function of perifused rat corpora lutea.

Corpora lutea (CL) from synchronously ovulated, prepubertal rats remained viable in perifusing Eagle medium (Dulbecco modified) flowing at a rate of 1 ml/hr. As an index of viability, progesterone (P) and 20alpha-hydroxypreng-4-ene-3-one (20alpha-OH-P) were determined by radioimmunoa-say in effluent media. Corpora lutea removed on the 2nd day of diestrus (D-2) or the 2nd day of pseudopregnancy (PP-2) showed fatigue by a continuous decline in the concentrations of effluent progestins.

Ultrastructural studies in epidermolysis bullosa heriditaria. I. Dominant dystrophic type of Pasini.

Ultrastructural examination was performed in 8 biopsies from 4 patients with the Pasini type of epidermolysis bullosa dystrophica dominans. The biopsies were taken from: 1. clinically normal skin from nonpredilection areas, 2.