Publications by authors named "Haruya Yagishita"
Neurons generate various spike patterns to execute different functions. Understanding how these physiological neuronal spike patterns are related to their molecular characteristics is a long-standing issue in neuroscience. Herein, we review the results of recent studies that have addressed this issue by integrating physiological and transcriptomic techniques.
View Article and Find Full Text PDFHippocampal pyramidal neurons exhibit diverse spike patterns and gene expression profiles. However, their relationships with single neurons are not fully understood. In this study, we designed an electrophysiology-based experimental procedure to identify gene expression profiles using RNA sequencing of single hippocampal pyramidal neurons whose spike patterns were recorded in living mice.
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- Hypotaurine, a key antioxidant found in fetal plasma and the placenta, is linked to fetal growth, particularly in ezrin-knockout mice where its levels are low.
- The study measured the expression of hypotaurine transporters, particularly GAT2, in the placenta throughout mid-to-late gestation and discovered that GAT2 levels increased significantly in this period.
- Results indicated that GAT2 is crucial for the uptake of hypotaurine from fetal circulation, primarily located in the syncytiotrophoblasts of the placenta, which could influence fetal development.
Article Synopsis
- Astrocytes play a vital role in clearing glutamate from synapses, but the specific functioning of their transporters around synapses is not fully understood.
- Research shows that the Down syndrome cell adhesion molecule (DSCAM) in Purkinje cells is crucial for proper synapse formation and function in the cerebellum, with defects observed in Dscam-mutant mice.
- This study highlights the importance of the interaction between synaptic proteins and astrocytic transporters, indicating that DSCAM is essential for motor learning, although it doesn’t affect gross motor coordination.
The ventral hippocampus (vHC) is a core brain region for emotional memory. Here, we examined how the vHC regulates stress susceptibility from the level of gene expression to neuronal population dynamics in male mice. Transcriptome analysis of samples from stress-naïve mice revealed that intrinsic calbindin (Calb1) expression in the vHC is associated with susceptibility to social defeat stress.
View Article and Find Full Text PDFUrethane, an anesthetic utilized for animal experiments, induces neocortical slow oscillations in which a large number of neurons emit rhythmic synchronized activity. However, it remains unclear how urethane affects neuronal activity in the hippocampus. In this study, we obtained in vivo patch-clamp recordings from dorsal hippocampal CA1 neurons in mice and found a reduction in the fluctuation of subthreshold membrane potentials during urethane anesthesia, implying reduced synaptic activity in the hippocampus.
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