Intramanchette transport during primate spermiogenesis: expression of dynein, myosin Va, motor recruiter myosin Va, VIIa-Rab27a/b interacting protein, and Rab27b in the manchette during human and monkey spermiogenesis.

Aim: To show whether molecular motor dynein on a microtubule track, molecular motor myosin Va, motor recruiter myosin Va, VIIa-Rab27a/b interacting protein (MyRIP), and vesicle receptor Rab27b on an F-actin track were present during human and monkey spermiogenesis involving intramanchette transport (IMT).

Methods: Spermiogenic cells were obtained from three men with obstructive azoospermia and normal adult cynomolgus monkey (Macaca fascicularis). Immunocytochemical detection and reverse transcription-polymerase chain reaction (RT-PCR) analysis of the proteins were carried out.

Uterine relaxation by sacral surface electrical stimulation on the day of embryo transfer.

The peak power of uterine peristalses after sacral surface electrical stimulation (ssES) treatment decreased markedly in comparison with that measured before ssES on the day of embryo transfer (ET). This suppression effect of uterine peristalses was stronger on day 5 of ET than on day 3. Because the uterus at the time of ET is sensitive to ssES, this electrical neuromodulation may be an effective method to induce uterine relaxation for ET.

Studies on the human leukocyte antigens in patients with polycystic ovary syndrome in a Japanese population--possible susceptibility of HLA-A11 and -DRB1*0403 to patient population with polycystic ovary syndrome.

Problem: The objective of this study was to identify the human leukocyte antigen (HLA) alleles that confer susceptibility or resistance to polycystic ovary syndrome (PCOS) in the Japanese population.

Method Of Study: HLA-A, -B and -C antigens were determined in 56 patients with PCOS using conventional serological method. HLA-DRB1 genotypes were determined using a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method in 68 patients with PCOS.

Prognostic factors for ovulatory response with clomiphene citrate in polycystic ovary syndrome.

Objective: To evaluate prospectively the prognostic factors for ovulatory responses following clomiphene citrate (CC) administration in polycystic ovary syndrome (PCOS).

Study Design: Fifty-nine infertile patients with a diagnosis of PCOS were recruited. Ovulation was induced using 100 mg/day CC administered daily from days 5 to 9 of the cycle.

Dynamic changes in the cytoskeleton during human spermiogenesis.

Objective: To investigate the structural changes in the cytoskeleton (microtubules, microfilaments) and examine the expression of centrosomal functional proteins during human spermiogenesis.

Design: Immunofluorescent staining of human spermiogenic cells.

Setting: University hospital and IVF clinic.