A novel resveratrol derivative selectively inhibits the proliferation of colorectal cancer cells with KRAS mutation.

Resveratrol is a polyphenolic compound in many edible foods including grapes, peanuts, and berries. Several studies have revealed the beneficial effects of resveratrol against various diseases such as heart disease, diabetes, obesity, neurological disorders, and cancer. A recent study showed that resveratrol inhibits the proliferation of HCT116 human colorectal cancer cells in three-dimensional culture (3DC) via induction of luminal apoptosis in HCT116 cell spheroids.

Long Noncoding RNA PANDA Positively Regulates Proliferation of Osteosarcoma Cells.

Background: A long noncoding RNA, p21-associated ncRNA DNA damage-activated (PANDA), associates with nuclear transcription factor Y subunit alpha (NF-YA) and inhibits its binding to promoters of apoptosis-related genes, thereby repressing apoptosis in normal human fibroblasts. Here, we show that PANDA is involved in regulating proliferation in the U2OS human osteosarcoma cell line.

Materials And Methods: U2OS cells were transfected with siRNAs against PANDA 72 h later and they were subjected to reverse transcription-polymerase chain reaction (RT-PCR), quantitative RT-PCR and cell-cycle analysis.

ANRIL regulates the proliferation of human colorectal cancer cells in both two- and three-dimensional culture.

ANRIL is a long noncoding RNA transcribed from the INK4 locus that encodes three tumor suppressor genes, p15, p16, and ARF. Previous studies demonstrated that ANRIL represses p15 and p16, which positively regulate the pRB pathway, leading to repression of cellular senescence of human normal fibroblasts. However, the role of ANRIL in cancer cell proliferation is less well understood.

Long Noncoding RNA ANRIL Regulates Proliferation of Non-small Cell Lung Cancer and Cervical Cancer Cells.

Background: Long noncoding RNA ANRIL (antisense non-coding RNA in the INK4 locus) represses p15 and p16, which induce cell-cycle arrest at G1 phase, leading to enhanced cell proliferation of normal fibroblasts. Herein we report that ANRIL is also involved in the regulation of cancer-cell proliferation.

Materials And Methods: HeLa and H1299 cells were transfected with ANRIL siRNAs.

Transcriptional Regulation of the p16 Tumor Suppressor Gene.

The p16 tumor suppressor gene encodes a specific inhibitor of cyclin-dependent kinase (CDK) 4 and 6 and is found altered in a wide range of human cancers. p16 plays a pivotal role in tumor suppressor networks through inducing cellular senescence that acts as a barrier to cellular transformation by oncogenic signals. p16 protein is relatively stable and its expression is primary regulated by transcriptional control.

Laterality of asymmetry in movements of the corners of the mouth during voluntary smile.

Objectives: To examine (1) the laterality of asymmetry in movements of the right and left corners of the mouth in space during voluntary smile and (2) the laterality of asymmetry in relation to the difference between the right and left hemiface size and the handedness.

Materials And Methods: Participants were 155 volunteer Japanese female adults. They were categorized into the symmetric group (n = 120) and the right-side hemiface dominant group (n = 26) according to the hemiface size.