Rational Design of the Soluble Variant of l-Pipecolic Acid Hydroxylase using the α-Helix Rule and the Hydropathy Contradiction Rule.

The production of recombinant proteins in is an important application of biotechnology. 2-Oxoglutarate-dependent l-pipecolic acid hydroxylase derived from (XdPH) is an excellent biocatalyst that catalyzes the hydroxylation of l-pipecolic acid to produce -5-hydroxy-l-pipecolic acid. However, the enzyme tends to form aggregates in the expression system.