A comparative study of sequential and simultaneous enzymatic and ultrasound-assisted aqueous two-phase extraction for anticholesterol compounds from Strobilanthes crispus leaves.

An innovative ultrasonic-assisted enzymatic aqueous two-phase extraction (UAE-ATPE) method was applied to enhance the yield from Strobilanthes crispus leaves, exploring both sequential and simultaneous approaches. Comparative analysis included assessing total phenolic content (TPC), total flavonoid content (TFC), partition coefficient (k) and recovery (R). Liquid chromatography-mass spectrometry and scanning electron microscopy evaluated extracts from both techniques.