Publications by authors named "Harsimardeep S Gill"

End-use and processing traits in wheat (Triticum aestivum L.) are crucial for varietal development but are often evaluated only in the advanced stages of the breeding program due to the amount of grain needed and the labor-intensive phenotyping assays. Advances in genomic resources have provided new tools to address the selection for these complex traits earlier in the breeding process.

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Multi-environmental characterization of flag leaf morphology traits in the US winter wheat revealed nine stable genomic regions for different flag leaf-related traits including a major region governing flag leaf angle. Flag leaf in wheat is the primary contributor to accumulating photosynthetic assimilates. Flag leaf morphology (FLM) traits determine the overall canopy structure and capacity to intercept the light, thus influencing photosynthetic efficiency.

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Integrating high-throughput phenotyping (HTP) based traits into phenomic and genomic selection (GS) can accelerate the breeding of high-yielding and climate-resilient wheat cultivars. In this study, we explored the applicability of Unmanned Aerial Vehicles (UAV)-assisted HTP combined with deep learning (DL) for the phenomic or multi-trait (MT) genomic prediction of grain yield (GY), test weight (TW), and grain protein content (GPC) in winter wheat. Significant correlations were observed between agronomic traits and HTP-based traits across different growth stages of winter wheat.

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Article Synopsis
  • Fusarium head blight (FHB) is a damaging wheat disease that decreases yield and quality, making it critical to develop resistant cultivars, but current phenotyping methods are biased and costly.
  • The study utilized AI-based techniques for accurate estimation of Fusarium-damaged kernels (FDK) and showed that this approach significantly improved predictive ability for deoxynivalenol (DON) in genomic selection models.
  • Additionally, hyperspectral imaging combined with deep learning further refined predictions for DON, indicating that AI and advanced imaging can effectively enhance trait selection in FHB-affected wheat.
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Powdery mildew is a devastating disease that affects wheat yield and quality. Wheat wild relatives represent valuable sources of disease resistance genes. Cloning and characterization of these genes will facilitate their incorporation into wheat breeding programs.

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Article Synopsis
  • * The resistance gene Pm13 was identified and cloned from the wild wheat Aegilops longissima, encoding a mixed lineage kinase domain-like (MLKL) protein.
  • * Research validated Pm13's function and developed wheat lines with this gene to improve resistance against powdery mildew, enhancing understanding of wheat immunity.
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Improvement of end-use quality remains one of the most important goals in hard winter wheat (HWW) breeding. Nevertheless, the evaluation of end-use quality traits is confined to later development generations owing to resource-intensive phenotyping. Genomic selection (GS) has shown promise in facilitating selection for end-use quality; however, lower prediction accuracy (PA) for complex traits remains a challenge in GS implementation.

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Leaf rust, caused by () is among the most devastating diseases posing a significant threat to global wheat production. The continuously evolving virulent races in North America calls for exploring new sources of leaf rust resistance. A diversity panel of 365 bread wheat accessions selected from a worldwide population of landraces and cultivars was evaluated at the seedling stage against four races (TDBJQ, TBBGS, MNPSD and, TNBJS).

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A better understanding of the genetic control of spike and kernel traits that have higher heritability can help in the development of high-yielding wheat varieties. Here, we identified the marker-trait associations (MTAs) for various spike- and kernel-related traits in winter wheat (Triticum aestivum L.) through genome-wide association studies (GWAS).

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Fusarium head blight (FHB), caused by the fungus Schwabe is an important disease of wheat that causes severe yield losses along with serious quality concerns. Incorporating the host resistance from either wild relatives, landraces, or exotic materials remains challenging and has shown limited success. Therefore, a better understanding of the genetic basis of native FHB resistance in hard winter wheat (HWW) and combining it with major quantitative trait loci (QTLs) can facilitate the development of FHB-resistant cultivars.

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Article Synopsis
  • * The research identified 17 significant marker-trait associations (MTAs) related to these traits, uncovering 12 potential quantitative trait loci (QTLs), including some novel QTLs on chromosomes 1A, 5B, and 7A.
  • * Findings from a larger analysis of 1,124 accessions aid breeders in selecting favorable alleles and techniques for more effective wheat breeding, enhancing yield through marker-assisted selection.
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Genomic prediction is a promising approach for accelerating the genetic gain of complex traits in wheat breeding. However, increasing the prediction accuracy (PA) of genomic prediction (GP) models remains a challenge in the successful implementation of this approach. Multivariate models have shown promise when evaluated using diverse panels of unrelated accessions; however, limited information is available on their performance in advanced breeding trials.

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Stagonospora nodorum blotch (SNB) is an economically important wheat disease caused by the necrotrophic fungus Parastagonospora nodorum. SNB resistance in wheat is controlled by several quantitative trait loci (QTLs). Thus, identifying novel resistance/susceptibility QTLs is crucial for continuous improvement of the SNB resistance.

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Successful seedling establishment depends on the optimum depth of seed placement especially in drought-prone conditions, providing an opportunity to exploit subsoil water and increase winter survival in winter wheat. Coleoptile length is a key determinant for the appropriate depth at which seed can be sown. Thus, understanding the genetic basis of coleoptile length is necessary and important for wheat breeding.

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Leaf rust caused by Eriks is one of the most problematic diseases of wheat throughout the world. The gene confers effective resistance against leaf rust at both seedling and adult plant stages. Previous studies had reported to be both recessive and dominant in hexaploid wheat; however, in diploid (TA2450), we found to be dominant by studying segregation in two independent F and their F populations.

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