α-Synuclein is the major platelet isoform but is dispensable for activation, secretion, and thrombosis.

Platelets play many roles in the vasculature ensuring proper hemostasis and maintaining integrity. These roles are facilitated, in part, by cargo molecules released from platelet granules via oluble SF ttachment rotein eceptor (SNARE) mediated membrane fusion, which is controlled by several protein-protein interactions. Chaperones have been characterized for t-SNAREs (.

The complementary roles of VAMP-2, -3, and -7 in platelet secretion and function.

Platelet secretion requires Soluble N-ethylmaleimide Sensitive Attachment Protein Receptors (SNAREs). Vesicle SNAREs/Vesicle-Associated Membrane Proteins (v-SNAREs/VAMPs) on granules and t-SNAREs in plasma membranes mediate granule release. Platelet VAMP heterogeneity has complicated the assessment of how/if each is used and affects hemostasis.

Protocol for optimizing production and quality control of infective EcoHIV virions.

EcoHIV is a model of HIV infection that recapitulates aspects of HIV-1 pathology in mice. However, there are limited published protocols to guide EcoHIV virion production. Here, we present a protocol for producing infective EcoHIV virions and essential quality controls.

Platelet α-granule cargo packaging and release are affected by the luminal proteoglycan, serglycin.

Background: Serglycin (SRGN) is an intragranular, sulfated proteoglycan in hematopoietic cells that affects granule composition and function.

Objective: To understand how SRGN affects platelet granule packaging, cargo release, and extra-platelet microenvironments.

Methods: Platelets and megakaryocytes from SRGN mice were assayed for secretion kinetics, cargo levels, granule morphology upon activation, and receptor shedding.

Brewers' spent grain in adsorption of aqueous Congo Red and malachite Green dyes: Batch and continuous flow systems.

Sorption of Congo Red (CR) and Malachite Green (MG) dyes currently used in pigments and clothing industries were investigated using brewers' spent grain (BSG) from a local brewery. Adsorption increased with a higher adsorbent weight and lower colorant concentrations. Accumulation of CR and MG was optimal at acidic pH and neutral pH respectively.