Publications by authors named "Harrison Tidwell"
Article Synopsis
- Flow cytometry is a technique for analyzing immune cells, and spectral flow cytometry enhances this by allowing for high-dimensional analysis, addressing the limitations of traditional methods.
- A novel analysis pipeline was developed to better handle data from large antibody panels, helping to identify rare T cell populations in aging by applying advanced methods like dimensionality reduction and unsupervised clustering.
- The study compared T cell clusters from young and aged mice, revealing significant age-related differences in T cell subsets, and demonstrated the pipeline's effectiveness with human PBMC datasets, potentially paving the way for new therapeutic targets in age-related immune issues.
Flow cytometry is a widely used technique for immune cell analysis, offering insights into cell composition and function. Spectral flow cytometry allows for high-dimensional analysis of immune cells, overcoming limitations of conventional flow cytometry. However, analyzing data from large antibody panels can be challenging using traditional bi-axial gating strategies.
View Article and Find Full Text PDFAging is a strong risk factor for atherosclerosis and induces accumulation of memory CD8 T cells in mice and humans. Biological changes that occur with aging lead to enhanced atherosclerosis, yet the role of aging on CD8 T cells during atherogenesis is unclear. In this study, using femle mice, we found that depletion of CD8 T cells attenuated atherogenesis in aged, but not young, animals.
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