Publications by authors named "Harrison B Rose"
Article Synopsis
- - Understanding how amyloid proteins like Sup35NM aggregate is crucial for studying amyloid diseases, and recent research has used thioflavin T fluorescence to track the formation of amyloid fibrils.
- - The study monitored the mass of aggregates during fibril formation under different conditions, revealing that multiple processes like primary nucleation, monomeric elongation, secondary nucleation, and fragmentation could be involved, but their relative significance remains unclear.
- - A new framework using a population balance model (PBM) was developed to analyze aggregation kinetics and fibril length distributions, suggesting that secondary nucleation contributes less to the generation of new fibrils compared to fragmentation, and integrating both kinetic data and size distribution can enhance understanding of aggregation mechanisms.
Determination of an equilibrium pH value in complex aqueous solution and deconvolution of this equilibrium to evaluate phenomena related to mixing, dilution, or progress of reaction is increasingly important in areas ranging from water quality to pharmaceutical formulations and manufacturing. Linearization of pH problems by simple algebraic substitution enables equilibria within complex buffered aqueous solutions to be modeled as an eigenvalue problem. This formulation approach makes rigorous determination of equilibrium pH values and reactor dynamics more accessible than with previous calculation methods, even when activity coefficients and non-ideality are considered.
View Article and Find Full Text PDFResearch involving α/β hydrolases, including α-amino acid ester hydrolase and cocaine esterase, has been limited by the lack of an online high throughput screening assay. The development of a high throughput screening assay capable of detecting α/β hydrolase activity toward specific substrates and/or chemical reactions (e.g.
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