Background: Endotoxemia is a common and severe disease of horses. Most previous studies have monitored changes caused by a bolus dose of endotoxin over short time periods.
Objectives: We aimed to describe inflammatory responses to endotoxin with inflammatory and hematologic markers monitored over a longer time than has been performed in the past using more prolonged endotoxin exposures.
In this case report, a Swedish flat-coated retriever was diagnosed with an extensive Hepatozoon canis infection. The dog had a prominent monocytosis (14.0 × 10 /L) with H canis gamonts detected in most monocytes, but none were found in the neutrophils.
View Article and Find Full Text PDFBackground: Neutrophil myeloperoxidase content is determined by the Advia 2120 hematology system by staining characteristics. Changes in myeloperoxidase staining are shown by location of neutrophils on Advia peroxidase dot plots and as myeloperoxidase index (MPXI). Significant changes in MPXI have been reported during severe inflammation in horses, dogs, and people but conclusions were inconsistent.
View Article and Find Full Text PDFBackground: Prostaglandin E1 (PGE1) and Iloprost inhibit platelet aggregation and should prevent or minimize preanalytic error with feline platelet enumeration.
Objectives: The objective was to compare the relative effectiveness in reducing errors in platelet enumeration by adding Iloprost to feline EDTA blood specimens in comparison to adding PGE1 or EDTA alone. In addition, a grading system for platelet aggregation in blood smears was evaluated for effectiveness in predicting prominent errors and compared to ADVIA's PLT-CLM flag.
Background: The performance of a digital Atago PAL-USG Cat refractometer (Atago) was compared with a Schmidt and Haensch, Goldberg type refractometer (S+H).
Materials And Methods: Specific gravity of 47 canine and feline urine samples was determined with both refractometers and the results were compared with Passing-Bablok and Bland-Altman plots. In addition, the specific gravity of dilutions of 10% glucose, 10% NaCl, and 3% albumin solutions was determined and compared with expected values.
A severe regenerative anemia was detected in a 12-week-old mixed breed puppy in Sweden. A small protozoan parasite was observed in erythrocytes on a blood smear. It was initially suspected to be Babesia gibsoni based on its size and because B.
View Article and Find Full Text PDFA manual method (Thrombo-TIC; Bioanalytic GmbH, Umkirch/Freiburg, Germany) was advertised to disaggregate platelet clumps and to make human platelets spherical to improve platelet enumeration. The current study's hypothesis was that this method would perform better than current methods for feline blood anticoagulated with ethylenediamine tetra-acetic acid (EDTA), which often contains platelet aggregates. Platelet concentrations (PLTs) were determined in 21 feline blood samples by 3 methods.
View Article and Find Full Text PDFBackground: Determination of the plateletcrit (PCT) is the most effective way to evaluate platelet mass in dogs, such as Cavalier King Charles spaniel (CKCS) dogs, with macrothrombocytopenia. The IDEXX VetAutoread hematology analyzer, which performs quantitative buffy coat (QBC) analysis, has been validated to determine platelet mass in CKCS dogs. The Advia 2120 reports a PCT, but the validity of this value has not been evaluated for dogs with macrothrombocytopenia.
View Article and Find Full Text PDFBackground: Most automated hematology analyzers cannot detect canine or feline basophils. However, many veterinary laboratories continue to report basophils as part of the automated 5-part differential leukocyte count for dogs and cats.
Objectives: The study objectives were to evaluate the performance of the Sysmex XT-2000iV, Advia 2120, and CELL-DYN 3500 hematology analyzers in detecting basophils using blood from dogs, cats, and rabbits with basophilia and to investigate the concurrence of basophilia and other hematologic changes, sex, and breed in dogs.
Background: For differential leukocyte counts, automated blood smear evaluation systems have been too slow or inaccurate to replace or supplement the manual differential count. The CellaVision DM96Vision (DM96V), a new instrument, is an automated image analysis system that is rapid and accurate enough to be used for enumerating human leukocytes and may be useful for analysis of canine blood.
Objectives: The aims of this study were to evaluate the performance of the DM96V in differential counting of canine leukocytes, to compare its performance with that of other methods, and to analyze interoperator variability.
Seven, adult, female beagles were inoculated with a Swedish granulocytic Ehrlichia organism closely related to Ehrlichia equi and E. phagocytophila. Blood and bone marrow changes were evaluated throughout the acute phase of infection.
View Article and Find Full Text PDFHypernatremia in two cats and hyponatremia in a dog were associated with artifactual changes in red blood cell (RBC) indices and hematocrit (HCT) determined on a Bayer H*1 hematology analyzer. The RBC cytograms and histograms revealed a population shifted towards macrocytic, hypochromic RBC in the hypernatremic cats, and towards microcytic, hyperchromic RBC in the hyponatremic dog. Reference intervals for the difference between manual packed cell volume (PCV) and analyzer-derived HCT in normonatremic dogs, cats and horses were established.
View Article and Find Full Text PDFBackground: Delayed analysis of blood samples may be caused by restricted access to laboratories. Artifactual changes may occur in the measured analytes as a consequence of delayed analysis and may complicate interpretation of the data.
Objective: The purpose of this study was to characterize artifactual changes in equine blood, due to storage, using the Advia 120 hematology analyzer.
The differential leukocyte counts performed by an automated hematology analyzer, the Technicon H-1E Hematology System, and traditional microscopic method (M-Diff) from blood samples of 129 horses, 40 cattle, and 140 cats were compared. The comparison was repeated after selected subsets of data were created by deleting samples with certain patterns suggesting error with the automated differential cell count (A-Diff). The two methods had good comparison of results for neutrophils and lymphocytes in all three species.
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