EVALUATION OF GRA6 AS GENETIC MARKER FOR DETERMINING GENOTYPE IN THE CEREBROSPINAL FLUID OF HIV/AIDS PATIENTS WITH TOXOPLASMIC ENCEPHALITIS.

Background: Toxoplasmic encephalitis is a severe manifestation of infection, with potentially fatal outcomes, particularly among immunocompromised patients. Clinical manifestation of this infection is associated with a specific genotype of , requiring the use of genetic marker for genotype determination.

Aims: This study critically evaluated the application of GRA6 gene as genetic marker for genotyping in cerebrospinal fluid (CSF) samples from HIV/AIDS patients diagnosed with Toxoplasmic encephalitis.

Comparative GRA6 and GRA7 for their Utility as Genetic Markers in the Genotyping of Cerebral Toxoplasmosis in Cerebrospinal Fluid.

Introduction: Cerebral toxoplasmosis is a severe symptom of Toxoplasma gondii (T. gondii) infection that often affects individuals with Human Immunodeficiency Virus (HIV) infection and can be fatal. T.

Toxoplasma gondii SAG2 type III in an atypical presentation of ocular toxoplasmosis in Indonesia.

Objective: This study aimed to perform genotyping of Toxoplasma gondii strain or variant causing atypical toxoplasmic uveitis in Indonesian patients.

Methods: Ocular fluid samples originating from 46 uveitis patients with non-specific ocular manifestations were analysed for Toxoplasma infection by PCR of the B1 locus. The clonal type was determined by amplification, sequencing and phylogenetic analysis of SAG2 and GRA6 loci in B1-positive samples.