Prevalence of multidrug resistance in spp. isolated from wild bird feces in an urban aquatic environment.

Antimicrobial resistance (AMR) has been detected in the microbiota of wildlife, yet little is known about the origin and impact within the ecosystem. Due to the shortage of nonepizootic surveillance, there is limited understanding of the natural prevalence and circulation of AMR bacteria in the wild animal population, including avian species. In this surveillance study, feces from wild birds in proximity to the River Cam, Cambridge, England, were collected and spp.

Insights into the response of mangrove sediment microbiomes to heavy metal pollution: Ecological risk assessment and metagenomics perspectives.

Rapid urbanisation and ensuing anthropogenic pollution lead to an escalated occurrence of heavy metals and metal-resistant bacteria in the soil ecosystem. Mangrove ecosystems are particularly vulnerable to heavy metal bioaccumulation and often act as metal sinks of the coastal areas. As a consequence, the microbial population in mangrove sediments develop multifarious metal tolerance mechanisms to combat metal toxicity.

Metagenomic data on bacterial diversity profiling of Arabian sea sediment by amplicon sequencing.

This data is about the microbial community genome analysis of Arabian sea sediment by Illumina sequencing by targeting the hypervariable region V3 of 16S rRNA gene. The data analysis revealed the existence of numerous unknown sequences, indicating a large unexploited bacterial diversity in the area. The raw sequence data used for analysis is available in NCBI under the Sequence Read Archive (SRA) with the BioProject No.

Molecular cloning and characterization of a halotolerant α-amylase from marine metagenomic library derived from Arabian Sea sediments.

Functional screening of a metagenomic library of marine sediment revealed an amylolytic clone BTM109. This report states the purification and characterization of a moderately halotolerant α-amylase, with more than 51% activity in 2.5 M NaCl.

Evaluation of five in situ lysis protocols for PCR amenable metagenomic DNA from mangrove soils.

Microbes in nature are rarely amenable to growth by standard microbiological methods, with the majority being unculturable. Metagenomic methods help to bypass and overcome the limitations of traditional culturing method; wherein total community DNA is isolated, cloned into suitable vector and host systems. However, isolation of total community DNA itself remains a challenge.