Publications by authors named "Harish Madhav"
Article Synopsis
- The study investigates how stevioside and its metabolite, steviol, enhance glucose absorption by mimicking insulin activity in cells, focusing on their effects on glucose transporter (GLUT4) levels.
- The research employs in-vitro cell line models, measuring GLUT4 gene expression and protein levels through qPCR and ELISA, alongside assessing glucose uptake.
- Results demonstrate that both stevioside and steviol significantly increase GLUT4 expression and glucose uptake in specific cell lines, supporting their potential as effective anti-hyperglycemic agents.
Bromelain (BRM) is a defense protein present in the fruit and stem of pineapple (Ananas comosus) and it is grouped as a cysteine protease enzyme with diversified medicinal uses. Based on its therapeutic applications, bromelain has got sufficient attention in pharmaceutical industries. In the present study, the full coding gene of bromelain in pineapple stem (1,093 bp) was amplified by RT-PCR.
View Article and Find Full Text PDFThe sweetness of honey leaf plant Stevia rebaudiana is attributed to steviol glycosides or steviosides, accumulated in the leaves. Steviol glycosides are diterpenoids derived from steviol as the final step of glycosylation by the marker enzyme Uridine diphosphate glycosyltransferase (UGT). Out of the eight different steviol glycosides, rebaudioside A was detected as the sweetest glycoside with reduced bitter aftertaste.
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