Publications by authors named "Harika Gadeela"
Introduction: Heat stress poses a significant environmental challenge, impacting plant growth, diminishing crop production, and reducing overall productivity. Plants employ various mechanisms to confront heat stress, and their ability to survive hinges on their capacity to perceive and activate appropriate physiological and biochemical responses. One such mechanism involves regulating multiple genes and coordinating their expression through different signaling pathways.
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- - The CRISPR/Cas9 technology is being applied to improve grain legumes like pigeonpea and groundnut for better genetic modification, but has faced difficulties in achieving efficient gene editing and transformation rates. - This study focuses on targeting the phytoene desaturase (PDS) gene, critical for carotenoid production, by using Agrobacterium-mediated transformation methods to develop constructs for both pigeonpea and groundnut. - Successful transformations yielded efficiencies of 15.2% for pigeonpea and 20% for groundnut, resulting in mutations that caused albino phenotypes due to specific nucleotide changes, providing a strong basis for further advancements in genome editing for legume crops.