Effect of Excess Fluoride Exposure on Radiographic and Histopathological Changes in Long Bones of Rabbit.

The present study aimed to investigate the effects of excess fluoride exposure on long bones in young rabbits (Oryctolagus cuniculus). New Zealand White rabbits (n = 30) were randomly divided into five equal groups and were provided drinking water containing 0, 50, 100, 200, and 400 µg added fluoride per ml ad lib for a period of 90 days. Blood samples were collected on days 0, 45, and 90 of the experiment, and femur samples were collected for fluoride estimation on day 90 after radiography of long bone before sacrifice.

Biomechanical properties of a novel locking compression plate to stabilize oblique tibial osteotomies in buffaloes.

Objective: Quantitation of mechanical properties of a novel locking compression plate (LCP) for bovine tibia.

Study Design: Prospective in vitro study.

Sample Population: Adult Murrah buffalo (250-350 kg) tibiae.

Allogeneic mesenchymal stem cells and growth factors in gel scaffold repair osteochondral defect in rabbit.

An attempt was made to improve osteochondral healing with allogeneic mesenchymal stem cells (MSCs) along with certain growth factors. Induced knee osteochondral defects were filled as: phosphate buffer saline (group A); MSCs in collagen gel (group B); group B plus insulin like growth factor-1 (group C); group C plus transforming growth factor β-1 (group D). Gross and scanning electron microscopy showed superior morphology and surface architecture of the healed tissue in groups D and C.

Mesenchymal stem cells with IGF-1 and TGF- β1 in laminin gel for osteochondral defects in rabbits.

Objective: Healing of articular cartilage is still a challenge due to its limited potential to regenerate. In the present study, we evaluated allogenic bone marrow mesenchymal stem cells (BM-MSCs) alone or in combination with growth factors, insulin-like growth factor-1 (IGF-1) and transforming growth factor-β1 (TGF-β1) in laminin scaffolds for healing of osteochondral defects.

Design: Osteochondral defects of 4mm (diameter) x 5mm (depth) were induced in the rabbit knee joints and treated with phosphate-buffered saline (PBS; control), BM-MSCs, BM-MSCs in laminin, BM-MSCs in laminin with IGF-1, or BM-MSCs in laminin with IGF-1 and TGF-β1 in 10 animals each.

Guinea pigs as an animal model for sciatic nerve injury.

The overwhelming use of rat models in nerve regeneration studies is likely to induce skewness in treatment outcomes. To address the problem, this study was conducted in 8 adult guinea pigs of either sex to investigate the suitability of guinea pig as an alternative model for nerve regeneration studies. A crush injury was inflicted to the sciatic nerve of the left limb, which led to significant decrease in the pain perception and neurorecovery up to the 4 weak.

Comparative evaluation of in vitro mechanical properties of different designs of epoxy-pin external skeletal fixation systems.

Objectives: To compare in vitro biomechanical properties of different designs of epoxy-pin external skeletal fixator (ESF) constructs.

Study Design: Mechanical testing study.

Sample Population: Four epoxy-pin ESF design constructs (uniplanar [EU], multiplanar-I [EM-I], multiplanar-II [EM-II], and circular [EC]) were mechanically tested in compression, bending, and torsion.

Effect of IGF-1 and Uncultured Autologous Bone-Marrow-Derived Mononuclear Cells on Repair of Osteochondral Defect in Rabbits.

Objective: To investigate the utility of bone-marrow-derived mononuclear cells (BMNCs) and insulin like growth factor-1 (IGF-1) in articular cartilage repair.

Design: An osteochondral defect of 3 mm diameter and 5 mm depth was created in patellar groove of the left knee joint in each of 36 New Zealand White rabbits. The defect was filled with RPMI-1640 medium in group A (control), autologous BMNCs in group B, and autologous BMNCs plus IGF-1 in group C (n = 12).

Management of tibial fractures using a circular external fixator in two calves.

Objectives: To report the repair of tibial diaphyseal fractures in 2 calves using a circular external skeletal fixator (CEF).

Study Design: Clinical report.

Animals: Crossbred calves (n=2; age: 6 months; weight: 55 and 60 kg).

Evaluation of two dynamic axial fixators for large ruminants.

Objectives: To evaluate healing of a radial osteotomy repaired by application of dynamic axial fixation devices (DAF) in large ruminants.

Study Design: In vivo study of bone healing after application of 2 DAF types. Model I had 2 sidebars, each with a central cylindrical cuff (internally threaded) with 2 detachable connecting rods telescoping within the cuff.

In vitro biomechanical properties of linear, circular, and hybrid external skeletal fixation devices for use in large ruminants.

Objective: To report the biomechanical properties of 3 external skeletal fixation (ESF) devices for use in large ruminants.

Study Design: In vitro biomechanical testing of ESF constructs.

Sample Population: Adult buffalo (weighing, 250-350 kg) tibiae (n=27).