Publications by authors named "Haq Ikram-Ul"
The aim of the present research was the efficient degradation of industrial textile wastewater dyes using a very active cloned laccase enzyme. For this purpose, potent laccase-producing bacteria were isolated from soil samples collected from wastewater-replenished textile sites in Punjab, Pakistan. The laccase gene from locally isolated strain LI-81, identified as , was cloned into vector pET21a, which was further transformed into BL21 codon plus.
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- Infective endocarditis (IE) is an infection affecting heart valves and implantable electronic devices, identified mainly through echocardiography.
- However, echocardiography has limitations in detecting issues related to prosthetic valves or devices, leading to increased use of cardiac CT and PET/CT scans.
- This review explores different guidelines on using multimodal imaging for diagnosing, managing complications, monitoring treatment, and predicting outcomes of IE.
Aims: Radiation therapy (RT) is an integral component of cancer therapy but associated with adverse events. Our goal was to establish risk prediction models for major adverse cardiovascular and cerebrovascular events (MACCE) after chest RT.
Methods And Results: A retrospective study of lung/breast cancer patients who had chest RT with planning CT at Mayo Clinic between 01/2010 and 01/2014.
Aim: This study aims to conduct a comprehensive analysis and scientometric evaluation of the top 100 most cited publications in the field of implant prosthodontics, authored by individuals affiliated with nations of the Middle East and North Africa (MENA) region.
Materials And Methods: In October 2023, the 100 most cited articles were gathered from the Web of Science database using the bibliometric research technique. The analysis was conducted on bibliometric indicators, including the distribution of articles over time, authorship, design of study, field of study, nature of research, contribution from various countries in MENA, international research collaboration, and most frequently used keywords by authors.
Glucose-derived acids for the further production of value-added medicine, food additives, and polymers, will promote lignocellulosic biomass biorefinery industry. In response to the diversity and complexity, a new method was established by employing high performance anion exchange chromatography (HPAEC) coupled with a CarboPac™ PA200 column, for the precise and fast determination of glucose, gluconic acid, glucuronic acid, 2-ketogluconic acid, 5-ketogluconic acid and glucaric acid. Based on the analysis of tiny varieties in retention behavior, a gradient elution mode was designed and optimized for the quantitative and qualitative analysis.
View Article and Find Full Text PDFRole of microbial laccases in valorization of lignocellulosic biomass to bioethanol.
Front Bioeng Biotechnol
July 2024
The persistent expansion in world energy and synthetic compounds requires the improvement of renewable alternatives in contrast to non-sustainable energy wellsprings. Lignocellulose is an encouraging feedstock to be utilized in biorefineries for its conversion into value-added products, including biomaterials, biofuels and several bio-based synthetic compounds. Aside from all categories, biofuel, particularly bioethanol is the most substantial fuel derived from lignocellulosic biomass and can be obtained through microbial fermentation.
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- * A significant increase in orthodontics research output was observed, with Arab nations contributing 5.02% to global research and experiencing a rise from 1.24% to 10.94% of publications.
- * Saudi Arabia was the most productive country, while collaboration with the U.S. was common; however, documents co-authored with Turkey had the highest citation impact, indicating an upward trend in research quality and collaboration.
Article Synopsis
- Hemicellulases are enzymes that break down hemicelluloses, which are common polysaccharides, and thermophilic hemicellulases from microbes are being researched for their potential in biofuel production due to their heat resistance and structural complexity.
- Recent studies are focused on understanding how these enzymes work, their stability at high temperatures, and their specific functions, which could lead to advancements in biotechnology and industrial applications.
- The article emphasizes the importance of improving thermophilic hemicellulases for eco-friendly catalysis and highlights challenges like high costs and the need for efficient bioprocesses using microbial consortia to make biofuel production more commercially viable.
Article Synopsis
- - Esterases are enzymes that break down ester bonds into water-soluble compounds and are widely used in various industries due to their commercial value and versatility.
- - Microbial esterases are preferred in industrial applications because they are cost-effective, provide high yields, and are not affected by seasonal changes.
- - As industries increasingly focus on eco-friendly practices, the review highlights the importance of bacterial esterases in various sectors, explores their structural properties and recent research advancements for optimizing their use.
Article Synopsis
- Okra is a widely consumed vegetable facing challenges from salinization and climate change, necessitating the development of salt-tolerant cultivars for sustainable production.
- A study evaluated 100 okra genotypes for salt tolerance by assessing their morphological and physio-biochemical responses under controlled and saline conditions.
- Results revealed significant genetic variability among genotypes, with five genotypes (95, 111, 133, 99, and 128) demonstrating both high yield and salt tolerance, essential for breeding efforts.
The excessive use of fossil has resulted in the drastic exhaustion of natural energy sources, leading to environmental challenges and energy crises. Owing to rising energy demand there is a dire need to shift towards renewable energies from lignocellulosic biomass. The present study assessed the co-production of biohydrogen (H) and biomethane (CH) by utilizing a less explored halophyte Various reaction parameters were evaluated for their effect on biohydrogen and biomethane production in batch experiments.
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- DNA polymerases are essential enzymes that replicate genetic information in nucleic acids, ensuring that every living cell can accurately copy its genomic DNA during cell division.
- Thermostable DNA polymerases are particularly valuable in biotechnology and molecular biology, facilitating techniques like DNA cloning, sequencing, and PCR.
- Research continues into the discovery and engineering of various DNA polymerases, with ongoing studies aimed at understanding their specialized functions and developing new applications for them.
Article Synopsis
- Despite advancements in health care, the rise of viral infections poses significant challenges in terms of health and financial burdens globally, particularly highlighted by over ten major epidemics in the 21st century, including COVID-19.
- The emergence of new viral strains and drug-resistant paths necessitates innovative therapeutic strategies, including the development of multi-target antiviral drugs inspired by natural resources and modern gene therapy techniques.
- The review covers the mechanisms of viral infections, advances in detection strategies, current approaches to combat these pathogens, their limitations, and novel potential treatment targets.
Atrial Appendage Aneurysms: Natural History and Outcomes.
World J Pediatr Congenit Heart Surg
July 2023
Background: Left (LAAA) and right atrial appendage aneurysms (RAAA) are rare entities, and their natural history, management, and long-term outcomes are not well studied.
Methods: This retrospective review includes all patients from 2000 to 2021 with atrial appendage aneurysms at our institution identified using an electronic search tool. LAAA and RAAA were confirmed using multimodality imaging and intraoperative findings.
Effect of pretreatment strategies on halophyte to improve saccharification using thermostable cellulases.
Front Bioeng Biotechnol
February 2023
Bioethanol is believed to be an influential revolutionary gift of biotechnology, owing to its elevating global demand and massive production. Pakistan is home to a rich diversity of halophytic flora, convertible into bounteous volumes of bioethanol. On the other hand, the accessibility to the cellulosic part of biomass is a major bottleneck in the successful application of biorefinery processes.
View Article and Find Full Text PDFCircular RNAs: Insights into Clinical and Therapeutic Approaches for Various Cancers.
Curr Protein Pept Sci
March 2023
Article Synopsis
- Circular RNAs (circRNAs) are unique noncoding RNAs formed through a process called exon back splicing, and they play crucial roles in regulating gene expression and cellular functions.
- Abnormal levels of circRNAs are commonly found in cancer, contributing to tumor growth, invasiveness, and evasion of cell death, which suggests their potential as clinical biomarkers and therapeutic targets.
- This review highlights the current understanding of circRNA functions, their promise in disease diagnosis and treatment, and the challenges that come with utilizing them in clinical settings.
Article Synopsis
- - Antibiotic resistance is a major global issue caused by overprescription, profit-driven healthcare, and inadequate research, leading to ineffective treatments and the spread of resistant bacteria.
- - Factors like antibiotic use in livestock, environmental impacts, and inadequate diagnostics contribute to rising resistance, particularly highlighted during the pandemic and in vulnerable groups like cancer patients.
- - New treatment alternatives, such as nanoparticles and bacteriophages, are being explored to address the increase in resistant pathogens, emphasizing the need for innovative solutions to combat this urgent health crisis.
Article Synopsis
- * These enzymes are widely found in animals, plants, and microorganisms, with microbial lipases being favored for industrial use due to lower production costs, higher yields, and easier purification methods.
- * Lipases play crucial roles across diverse industries, including food, pharmaceuticals, and biodiesel, but there are challenges in their commercial application that require further optimization and research to ensure their successful and sustainable use.
Article Synopsis
- * Keratinases are produced by thermophilic microorganisms through solid-state fermentation, leading to effective enzyme production with improved properties for various applications.
- * Advances in molecular techniques aim to enhance the productivity, activity, and stability of keratinases, making them valuable in pharmaceuticals, animal feed, fertilizers, biogas production, and more.
CRISPR/Cas9: A revolutionary genome editing tool for human cancers treatment.
Technol Cancer Res Treat
October 2022
Article Synopsis
- - Cancer results from genetic mutations and is a leading cause of global mortality. CRISPR/Cas9 is a powerful gene-editing technology originally found in bacteria that shows promise for cancer research and treatment.
- - CRISPR can advance cancer diagnosis by quickly detecting key genes related to cancer development and growth, and it plays a significant role in immunotherapy following traditional treatments like surgery and chemotherapy.
- - Despite its potential, CRISPR/Cas9 faces challenges like off-target effects, delivery issues, and low HDR efficiency that require further research to ensure effective clinical application in cancer therapies.
Purpose Of Review: To evaluate remote monitoring using implantable cardioverter-defibrillator (ICD) or cardiac resynchronization therapy defibrillator (CRT-D) devices as an adjunctive tool to the traditional care of patients with heart failure (HF).
Recent Findings: We included 11 trials encompassing 5965 patients. Absolute risk difference (ARD) with 95% credible interval (CrI) was estimated.
The effective treatment of waste to be used as a resource in future has a major role in achieving environmental sustainability and moving towards circular economy. The current research is aimed to provide in-depth detail regarding prominent trends and research themes, evolution, future research orientation, main characteristics, and mapping of research publications on waste management, technological innovation in circular economy domain from the year 2000 to 2021. Different analyses including text mining and bibliometric and content analyses were applied to answer the research question and provide the details on aforementioned variables.
View Article and Find Full Text PDFCisplatin induced vomiting involves multiple mechanisms in its genesis and a single antiemetic agent do not cover both the phases (acute & delayed) of vomiting in clinics; necessitating the use of antiemetics in combination. and other selected plants have ethnopharmacological significance in relieving emesis. The aim of the present study was to investigate the intrinsic antiemetic profile of (), family , and (, family ) in combinations against vomiting induced by highly emetogenic anticancer drug-cisplatin in pigeons.
View Article and Find Full Text PDFBackground: Heart block requiring permanent pacemaker (PPM) implantation is a relatively frequent complication of transcatheter aortic valve replacement (TAVR).
Objective: The purpose of this study was to perform a contemporary meta-analysis to provide an updated assessment of clinically useful predictors of PPM implantation post-TAVR.
Methods: Medline and EMBASE searches were performed to include all studies reporting PPM post-TAVR between 2015 and 2020.