Candida albicans Amphotericin B-Tolerant Persister Formation is Closely Related to Surface Adhesion.

Candida albicans persisters have so far been observed only in biofilm environment; the biofilm element(s) that trigger(s) persister formation are still unknown. In this study, we tried to further elucidate the possible relationship between C. albicans persisters and the early phases of biofilm formation, especially the surface adhesion phase.

[Dynamic and quantitative analysis of cariogenic bacteria and its proportion in the dental plaque of different caries-susceptible children].

Purpose: To investigate the ratio and change of bacteria in dental plaque in different caries-susceptible children.

Methods: Dental plaques were obtained from 60 children of 3-5 years old. Four targeted cariogenic bacteria were quantitatively detected by real-time PCR and the mean dmft score was calculated.

[The influence of starvation on Candida albicans biofilm and persisters formation].

Purpose: To investigate the influence of starvation on Candida albicans (C. albicans) biofilm and persisters formation.

Methods: The growth curve was made by CFU counts methods in different culture times to find the exponential and stationary phases.

[Dynamic monitoring and analysis of the persisters emerging in Candida albicans biofilm].

Purpose: To investigate the mechanism and approach related to persisters emerging in Candida albicans biofilm by studying its dynamic characteristics.

Methods: The Candida albicans biofilms model in vitro were formed by individual yeast cells and hyphal compartments respectively. The numbers of progenitive fungal cells and persisters at different stages were counted and analyzed with SPSS11.