Manganese-Modified Aluminum Adjuvant Enhances both Humoral and Cellular Immune Responses.

Aluminum adjuvants remain the most commonly used vaccine adjuvants. Being rather effective in triggering humoral immunity, however, aluminum adjuvants usually show limited abilities in activating cellular immunities. Herein, by adding manganese ions during the preparation of aluminum adjuvant, a manganese-modified aluminum (Mn-Al) adjuvant is obtained, which can effectively stimulate both humoral and cellular immune responses.

YOLO_Bolt: a lightweight network model for bolt detection.

Accurate, fast, and intelligent workpiece identification is of great significance to industrial production. To cope with the limited hardware resources of factory equipment, we have made lightweight improvements based on You Only Look Once v5 (YOLOv5) and proposed a lightweight YOLO named YOLO_Bolt. First, ghost bottleneck lightweight deep convolution is added to the backbone module and neck module of the YOLOv5 detection algorithm to reduce the model volume.

Coordination Tuning of Metal Porphyrins for Improved Oxygen Evolution Reaction.

The nucleophilic attack of water or hydroxide on metal-oxo units forms an O-O bond in the oxygen evolution reaction (OER). Coordination tuning to improve this attack is intriguing but has been rarely realized. We herein report on improved OER catalysis by metal porphyrin 1-M (M=Co, Fe) with a coordinatively unsaturated metal ion.

Metal-Corrole-Based Porous Organic Polymers for Electrocatalytic Oxygen Reduction and Evolution Reactions.

Integrating molecular catalysts into designed frameworks often enables improved catalysis. Compared with porphyrin-based frameworks, metal-corrole-based frameworks have been rarely developed, although monomeric metal corroles are usually more efficient than porphyrin counterparts for the electrocatalytic oxygen reduction reaction (ORR) and oxygen evolution reaction (OER). We herein report on metal-corrole-based porous organic polymers (POPs) as ORR and OER electrocatalysts.

Simultaneous Targeted Analysis of GGT and Its H-Type mRNA in HepG2 Cells Based on Degradable Silicon Nanomaterials.

Most important physiological processes in live cells are usually maintained by the interaction of multiple related biomolecules; the multi-target simultaneous analysis of these related molecules can better reflect the dynamic changes of their biological regulatory processes, providing more comprehensive information for diseases diagnosis and research. Herein, we have constructed the degradable multifunctional silica nanomaterials from the prepared degradable organic silicon source and further established degradable composite nanoprobes (DCNPs). The low toxicity of DCNPs could reduce the impact on normal physiological processes in cells and achieve the needs of living cell analysis applications; by the loading of the gamma-glutamyltransferase (GGT) activity-identification probe (Cy-GGT) and surface nucleic acid-recognizing molecular beacon (hairpin) modification, the DCNP realized the simultaneous image analysis of GGT and its related H-type mutated GGT mRNA (H-mRNA) in HepG2 cells and their quantitative detection in vitro.

A Green-Emission Metal-Organic Framework-Based Nanoprobe for Imaging Dual Tumor Biomarkers in Living Cells.

The modular nature of metal-organic frameworks (MOFs) permits their tunable structure and function for target application, such as in biomedicine. Herein, a green-emission Zr(IV)-MOF (BUT-88) was constructed from a customized luminescent carbazolyl ligand. BUT-88 represents the first bcu-type MOF with both organic linker and metal node in eight connections and shows medium-sized pores, rich accessible linking sites, and good water stability and biocompatibility.

Profiles of immune cell infiltration and immune-related genes in the tumor microenvironment of osteosarcoma.

This work aimed to investigate tumor-infiltrating immune cells (TIICs) and immune-associated genes in the tumor microenvironment of osteosarcoma. An algorithm known as ESTIMATE was applied for immune score assessment, and osteosarcoma cases were assigned to the high and low immune score groups. Immune-associated genes between these groups were compared, and an optimal immune-related risk model was built by Cox regression analyses.

Green-emitting carbon dot loaded silica nanoparticles coated with DNA-cross-linked hydrogels for sensitive carcinoembryonic antigen detection and effective targeted cancer therapy.

Herein, we have designed bifunctional composite nanospheres for carcinoembryonic antigen (CEA) sensing and targeted drug delivery, based on carbon dot loaded silica nanoparticles coated with DNA-cross-linked hydrogels. As a result, highly sensitive and selective CEA detection was achieved in vitro, and an effective cytotoxic effect was realized in vivo after loading doxorubicin.

A DNA Nanotube-Peptide Biocomplex for mRNA Detection and Its Application in Cancer Diagnosis and Targeted Therapy.

A biocomplex of DNA nanotube-peptide, consisting of six concatenated DNA strands, three locked DNA strands, and a cell-penetrating peptide, is reported. The barrel-structured DNA nanotube-peptide was successfully applied as a codrug-delivery system for targeting cancer therapy. The mucin 1 protein (MUC-1) aptamer is part of a DNA nanotube that can specifically recognize MUC-1 protein on the surface of MCF-7 cells.