Publications by authors named "Haoxian Ke"

Background: Tumour immune macroenvironment is comprised of tumour and surrounding organs responding to tumourigenesis and immunotherapy. The lack of comprehensive analytical methods hinders its application for prediction of survival and treatment response in colorectal cancer (CRC) patients.

Methods: Cytometry by time-of-flight (CyTOF) and RNA-seq was applied to characterise immune cell heterogeneity in a discovery cohort including tumour, blood and intestinal architecture comprising epithelium, lamina propria, submucosa, muscularis propria of normal bowel and tumour-adjacent bowel tissues.

View Article and Find Full Text PDF
Article Synopsis
  • The study investigates the role of methylenetetrahydrofolate dehydrogenase 1 (MTHFD1) in colorectal cancer (CRC), focusing on its impact on tumor growth and spread.
  • Using various laboratory techniques, researchers found that overexpression of MTHFD1 enhanced tumor growth and metastasis while decreasing autophagy in CRC cells, influenced by the PI3K-AKT-mTOR signaling pathway.
  • Conversely, reducing MTHFD1 levels led to increased autophagy and inhibited cell proliferation, migration, and invasion, suggesting MTHFD1 plays a crucial role in CRC progression.
View Article and Find Full Text PDF

The oncogenic role of KRAS in colorectal cancer (CRC) progression is well-established. Despite this, identifying effective therapeutic targets for KRAS-mutated CRC remains a significant challenge. This study identifies pyruvate dehydrogenase phosphatase catalytic subunit 1 (PDP1) as a previously unrecognized yet crucial regulator in the progression of KRAS mutant CRC.

View Article and Find Full Text PDF

Purpose: Immune checkpoint inhibitors (ICI) have become the standard of care for patients with mismatch repair-deficient/microsatellite instability-high (dMMR/MSI-H) colorectal cancer. However, biomarkers of response to ICI are still lacking.

Experimental Design: Forty-two patients with dMMR colorectal cancer treated with neoadjuvant PD-1 blockade were prospectively enrolled.

View Article and Find Full Text PDF
Article Synopsis
  • Many cancer patients show limited response to immunotherapies, but tumor-infiltrating cytotoxic T lymphocytes (CTL) may boost treatment effectiveness.
  • The study focused on identifying the role of the gene IFI35 in enhancing CD8 T cell activities and its potential benefits for CAR-T cell therapy targeting colorectal cancer.
  • Results indicated that higher IFI35 levels correlate with increased CD8 T cell presence and function, suggesting it could serve as a biomarker to improve CAR-T cell efficacy in colorectal cancer.
View Article and Find Full Text PDF

Background: Tumor heterogeneity is contributed by tumor cells and the microenvironment. Dynamics of tumor heterogeneity during colorectal cancer (CRC) progression have not been elucidated.

Methods: Eight single-cell RNA sequencing (scRNA-seq) data sets of CRC were included.

View Article and Find Full Text PDF

Immune checkpoint inhibitors (ICIs) represent a new paradigm in cancer immunotherapy, but can be largely restricted by the limited presence of CD8 cytotoxic T lymphocytes (CTLs) in colorectal cancer (CRC) patients with microsatellite stable (MSS) tumors. Here, through next-generation sequencing, we identify microtubule-associated protein 7 domain 2 (MAP7D2) as an exploitable therapeutic maneuver to improve the efficacy of ICIs for MSS CRC therapy. In human CRC tissues, MAP7D2 expression is significantly increased in MSS CRC, and MAP7D2 adversely correlates with the presence of antitumor T lymphocytes.

View Article and Find Full Text PDF

Long non-coding RNA (lncRNA) DANCR has been reported to participate in key processes such as stem cell differentiation and tumorigenesis. In a high throughput screening for lncRNAs involved in Doxorubicin-induced apoptosis, we found DANCR was suppressed by Doxorubicin and it acted as an important repressor of apoptosis in colorectal cancer. Further studies demonstrated that DANCR promoted the oncogenic lncRNA MALAT1 expression via enhancing the RNA stability of MALAT1 to suppress apoptosis.

View Article and Find Full Text PDF

Continent ileostomy (CI) was once a prevalent surgical technique for patients who required total proctocolectomy but then gave way to ileal pouch-anal anastomosis (IPAA) after 1980. Although IPAA has been the gold standard procedure preferred by most patients when total proctocolectomy is required, due to its imitation of physiological function of rectum and preserved function of anus, various complications have been observed with a relatively high rate of morbidity that could affect pouch longevity. Once serious complications such as pelvic abscesses and/or fistula occur, the pouch often needs to be removed.

View Article and Find Full Text PDF

A PHP Error was encountered

Severity: Warning

Message: fopen(/var/lib/php/sessions/ci_session3ibbea6evoolph11tbu6k5aqfvduh835): Failed to open stream: No space left on device

Filename: drivers/Session_files_driver.php

Line Number: 177

Backtrace:

File: /var/www/html/index.php
Line: 316
Function: require_once

A PHP Error was encountered

Severity: Warning

Message: session_start(): Failed to read session data: user (path: /var/lib/php/sessions)

Filename: Session/Session.php

Line Number: 137

Backtrace:

File: /var/www/html/index.php
Line: 316
Function: require_once