Chinese herbal medicine, Tongxieyaofang, alleviates diarrhea via gut microbiota remodeling: evidence from network pharmacology and full-length 16S rRNA gene sequencing.

Background: Tongxieyaofang (TXYF) was a traditional Chinese medicine (TCM) formula for the treatment of diarrhea with liver stagnation and spleen deficiency syndrome, but the potential targets and mechanisms have not been fully clarified. This study aims to explore the potential mechanisms of TXYF in alleviating diarrhea using network pharmacology and full-length 16S rRNA gene sequencing.

Methods: Network pharmacology was applied to identify bioactive compounds and potential targets involved in the role of TXYF in alleviating diarrhea.

Gut Microbiota Comparison Between Intestinal Contents and Mucosa in Mice With Repeated Stress-Related Diarrhea Provides Novel Insight.

Repeated stress-related diarrhea is a kind of functional bowel disorders (FBDs) that are mainly stemming from dysregulation of the microbiota-gut-brain axis mediated by a complex interplay of 5-hydroxytryptophan (5-HT). Intestinal content and intestinal mucosa microbiota belong to two different community systems, and the role of the two microbiota community systems in repeated stress-related diarrhea remains largely unknown. In order to ascertain the difference in composition and the potential function between intestinal content and intestinal mucosa microbiota response on repeated stress-related diarrhea, we collected intestinal contents and mucosa of mice with repeated stress-related diarrhea for 16S rRNA PacBio SMRT gene full-length sequencing, and with the digital modeling method of bacterial species abundance, the correlations among the two microbiota community systems and serum 5-HT concentration were analyzed.

Characteristics of intestinal microbiota and enzyme activities in mice fed with lily bulb.

The aim was to investigate the effect of lily bulbs on the microecological characteristics of intestinal microbiota and enzyme activities in normal mice. Thirty SPF Kunming mice were randomly divided into the control group, (LL) group and i var. unicolor (LDU) group.

Gut microbiota characteristics in mice with antibiotic-associated diarrhea.

Background: Antibiotic-associated diarrhea (AAD), defined as diarrhea that occurs in association with the administration of antibiotics and without another clear etiology, is one of the most common adverse drug events of antibiotics therapy. We established a diarrhea model induced by gentamycin and cefradine to investigate the microbiota characteristics in the intestinal lumen of mice with AAD and provide insights into noteworthy bacteria related to gentamicin and cefradine-associated diarrhea.

Results: The number of OTUs in the model group and the normal group was 983 and 2107, respectively, and 872 identical OTUs were shared between two groups.

Intestinal mucosal bacterial diversity of antibiotic-associated diarrhea (AAD) mice treated with and Qiweibaizhu powder.

The aim was to investigate the combined effect of and Qiweibaizhu powder (QWBZP) on the bacterial diversity of the intestinal mucosa of antibiotic-associated diarrhea (AAD) mice, for the potential treatment of diarrhea, especially which is induced by administration of antibiotics. Eighteen (18) mice were randomly assigned to three equal groups of six mice, namely Normal (mn group), Placebo control (mm group) and and QWBZP (DQ) treatment (mdq group). Mice were gavaged with a solution (23.

Microbiotal characteristics colonized in intestinal mucosa of mice with diarrhoea and repeated stress.

To understand the role of intestinal mucosal microbiota on mental stress-related diarrhoea, we collected the intestinal mucosa of mice treated with extract gavage combined with restraint and tail pinch stress for 7 days; and intestinal mucosal microbiota characteristics were analyzed by 16S rRNA Pacbio SMRT gene full-length sequencing. The results showed that the diversity (i.e.

Role of tryptophan-metabolizing microbiota in mice diarrhea caused by Folium sennae extracts.

Background: Although reports have provided evidence that diarrhea caused by Folium sennae can result in intestinal microbiota diversity disorder, the intestinal bacterial characteristic and specific mechanism are still unknown. The objective of our study was to investigate the mechanism of diarrhea caused by Folium sennae, which was associated with intestinal bacterial characteristic reshaping and metabolic abnormality.

Results: For the intervention of Folium sennae extracts, Chao1 index and Shannon index were statistical decreased.

Bacterial Diversity in the Intestinal Mucosa of Dysbiosis Diarrhea Mice Treated with Qiweibaizhu Powder.

The current research tried to explore the effect of Qiweibaizhu powder (QWBZP) on the bacterial diversity and community structure of the intestinal mucosa of dysbiosis diarrhea mice and provide a scientific basis for the efficacy of QWBZP on antibiotic-induced diarrhea. A dysbiosis diarrhea mouse model was constructed with broad-spectrum antibiotics through a mixture of cephradine capsules and gentamicin sulfate (23.33 mL·kg·d).