Structurally diverse alkaloids from the Buxus sinica and their cytotoxicity.

Extensive phytochemical study of the methanol extract of twigs and leaves of Buxus sinica resulted in the identification of forty-one Buxus alkaloids, including twenty undescribed ones, namely cyclobuxusinines A-I (1-7, 16 and 20), as well as secobuxusinines A-K (8-15 and 17-19). Their structures were delineated by detailed analysis using various spectroscopic techniques. cyclobuxusinines B (2) was the first Buxus alkaloid, whose CH-18 was oxidized, implying the presence of special oxidative enzymes in this plant.

Generation, degradation mechanism, and toxicity evaluation of pigmented compounds in Leucosceptrum canum nectar.

Leucosceptrum canum nectar (LCN) emerges as a novel food resource, distinguished by its unique dark brown hue. This study delves into the composition and toxicity assessment of novel pigments within LCN. Through liquid chromatography-tandem mass spectrometry (LC-MS/MS) and chemical synthesis, seventeen 2,5-di-(N-(-)-prolyl)-para-benzoquinone (DPBQ) analogs in LCN were identified.

Identification of New Diterpenoids from the Pulp of and Their α-Glucosidase Inhibition Activity.

Six new (, , , , , and ) and seven known (, , , , , , and ) diterpenoids have been identified in the pulp of . The structures of new diterpenoids were elucidated by extensive spectroscopic analysis, including 1D, 2D NMR (HSQC, HMBC, H-H COSY, and ROESY), HRESIMS, IR, DP4+, electronic circular dichroism, and X-ray crystallography analysis. Compound is -labdane-type diterpenoid, whereas compounds (-) are -kaurane diterpenoids.

Discovery of ACE Inhibitory Peptides Derived from Green Coffee Using In Silico and In Vitro Methods.

Inhibition of angiotensin-I converting enzyme (ACE) is an important means of treating hypertension since it plays an important regulatory function in the renin-angiotensin system. The aim of this study was to investigate the ACE inhibitory effect of bioactive peptides from green coffee beans using in silico and in vitro methods. Alcalase and thermolysin were employed to hydrolyze protein extract from coffee beans.