Four-Dimensional Data-Independent Acquisition-Based Proteomic Profiling Combined with Transcriptomic Analysis Reveals the Involvement of the Slym1-SlFHY3-CAB3C Module in Regulating Tomato Leaf Color.

In green plants, the chloroplast is responsible for light energy transition and organic assimilation. However, the molecular mechanisms underlying chloroplast development in horticultural crops remain unclear. Here, four-dimensional data-independent acquisition-based proteomic profiling identified 1,727 differentially expressed proteins between "Zhongshu 4" (ZS4) and () leaves, a considerable proportion of which were down-regulated chloroplast proteins.

Aspirin suppresses hepatocellular carcinoma progression by inhibiting platelet activity.

Background: Hepatocellular carcinoma (HCC) is the most common malignant liver disease in the world. Platelets (PLTs) are known to play a key role in the maintenance of liver homeostasis and the pathophysiological processes of a variety of liver diseases. Aspirin is the most classic antiplatelet agent.

LINC00330/CCL2 axis-mediated ESCC TAM reprogramming affects tumor progression.

Background: Tumor-associated macrophages (TAMs) significantly influence the progression, metastasis, and recurrence of esophageal squamous cell carcinoma (ESCC). The aberrant expression of long noncoding RNAs (lncRNAs) in ESCC has been established, yet the role of lncRNAs in TAM reprogramming during ESCC progression remains largely unexplored.

Methods: ESCC TAM-related lncRNAs were identified by intersecting differentially expressed lncRNAs with immune-related lncRNAs and performing immune cell infiltration analysis.

Anemonin ameliorates human diploid fibroblasts 2BS and IMR90 cell senescence by PARP1-NAD-SIRT1 signaling pathway.

Objective: Aging becomes the most predominant risk factor for all age-associated pathological conditions with the increase of life expectancy and the aggravation of social aging. Slowing down the speed of aging is considered an effective way to improve health, but so far, effective anti-aging methods are relatively lacking.

Methods: Anemonin (ANE) was screened from eight existing small-molecule compounds by cell viability assay.

An active tunable terahertz functional metamaterial based on hybrid-graphene vanadium dioxide.

In this paper, we propose a switchable and tunable functional metamaterial device based on hybrid graphene-vanadium dioxide (VO). Using the properties of the metal-insulator transition in VO, the proposed metamaterials can enable switching between tunable circular dichroism (CD) and dual-band perfect absorption in the terahertz region. When VO is in the insulator state, a polarization-selective single-band perfect absorption can be achieved for circularly polarized waves, thus resulting in a strong CD response with a maximum value of 0.

Escaping drought: The pectin methylesterase inhibitor gene Slpmei27 can significantly change drought resistance in tomato.

Drought stress will lead to a decrease in tomato yield and poor flavour, yield and quality, resulting in economic losses in agricultural production. Mining the key genes regulating tomato drought resistance is of great significance to improve the drought resistance of tomato plants. The cell wall can directly participate in the plant drought stress response as one of the main components of the cell wall, and the regulation of pectin content in plant drought resistance is still unclear.

Slym1 control the color etiolation of leaves by facilitating the decomposition of chlorophyll in tomato.

Photosynthesis, as an important biological process of plants, produces organic substances for plant growth and development. Although the molecular mechanisms of photosynthesis had been well investigated, the relationship between chlorophyll synthesis and photosynthesis remains largely unknown. The leaf-color mutant was an ideal material for studying photosynthesis and chlorophyll synthesis, which had been seldom investigated in tomato.

Dynamics of vaginal microbiome in female beagles at different ages.

Age-related changes in human vaginal microbiota composition have been reported, and such changes might be influenced by humidity, external stimuli, hormone levels, drug to use, and other factors. However, there is no report about the vaginal microbiota composition of female beagles at different ages. To investigate the effects of aging on the vaginal microbiota independent of other effects, we analyzed the vaginal microbiomes of 23 beagles at a wide range of ages from 1 year to 7 years old (except the 3rd year), 1-2 y were categorized into youth stage (YS), 4-5 y were categorized into middle stage (MS), and 6-7 y were categorized into elderly stage (ES) based on age.

Advantages of Lateral Flow Assays Based on Fluorescent Submicrospheres and Quantum Dots for Toxin B Detection.

colitis is caused by a cytotoxin produced by the anaerobic bacteria in the epithelial cells of the large intestine, particularly toxin B (TcdB). However, the sensitivity of currently utilized endotoxin determination methods has been called into question, and, therefore, more accurate and convenient detection methods are needed. Our study is the first to systematically compare fluorescent submicrosphere-based and quantum-dot nanobead-based lateral fluidity measurement methods (FMs-LFA and QDNBs-LFA) with toxin B quantification in fecal samples via sandwich analysis.

Morphological and anatomical characteristics of exserted stigma sterility and the location and function of SlLst (Solanum lycopersicum Long styles) gene in tomato.

Anatomical changes in and hormone roles of the exserted stigma were investigated, and localization and functional analysis of SlLst for the exserted stigma were performed using SLAF-BSA-seq, parental resequencing and overexpression of SlLst in tomato. Tomato accession T431 produces stigmas under relatively high temperatures (> 27 °C, the average temperature in Harbin, China, in June-August), so pollen can rarely reach the stigma properly. This allows the percentage of male sterility exceed 95%, making the use of this accession practical for hybrid seed production.

Rapid and fully-automated detection of Toxin B via magnetic-particle-based chemiluminescent immunoassay.

colitis is caused by a cytotoxin produced by the anaerobic bacteria on the epithelial cells of the large intestine, particularly toxin B (Tcd B). Current toxin assays have proven to be insensitive and have thus been ruled out from diagnostic purposes. Therefore, Tcd B detection via sandwich-type chemiluminescent immunoassay was proposed as a straightforward approach with potential diagnostic applicability.

The α-Subunit of the Chloroplast ATP Synthase of Tomato Reinforces Resistance to Gray Mold and Broad-Spectrum Resistance in Transgenic Tobacco.

Chloroplast ATP synthase (cpATPase) is responsible for ATP production during photosynthesis. Our previous studies showed that the cpATPase CF1 α subunit (AtpA) is a key protein involved in -induced resistance to the fungus in tomato. Here, we show that expression of the tomato gene was upregulated by and The tomato gene was then isolated, and transgenic tobacco lines were obtained.

Impact of biochar amendment on the uptake, fate and bioavailability of pharmaceuticals in soil-radish systems.

Crops grown in soils receiving wastewaters, biosolids, or manures can accumulate pharmaceuticals in edible parts, raising concerns over potential human exposure to multiple pharmaceuticals. Nonetheless, viable mitigation options for minimizing plant uptake of pharmaceuticals are limited. This study evaluated how biochar amendment could influence the uptake of 15 pharmaceuticals by radish (Raphanus sativus) grown in a sandy loam at two amendment rates (0.

The micropeptide LEMP plays an evolutionarily conserved role in myogenesis.

In recent years, micropeptides have been increasingly identified as important regulators in various biological processes. However, whether micropeptides are functionally conserved remains largely unknown. Here, we uncovered a micropeptide with evolutionarily conserved roles in myogenesis.

Endothelial CDS2 deficiency causes VEGFA-mediated vascular regression and tumor inhibition.

The response of endothelial cells to signaling stimulation is critical for vascular morphogenesis, homeostasis and function. Vascular endothelial growth factor-a (VEGFA) has been commonly recognized as a pro-angiogenic factor in vertebrate developmental, physiological and pathological conditions for decades. Here we report a novel finding that genetic ablation of CDP-diacylglycerol synthetase-2 (CDS2), a metabolic enzyme that controls phosphoinositide recycling, switches the output of VEGFA signaling from promoting angiogenesis to unexpectedly inducing vessel regression.

High-mobility group box 1 protein contributes to the immunogenicity of rTcdB-treated CT26 cells.

Clostridium difficile TcdB is a key virulence factor that causes C. difficile-associated diseases. Our previous studies have shown that recombinant full-length TcdB (rTcdB) induces cell death in CT26 cells, and rTcdB-treated CT26 cells with high immunogenicity could stimulate dendritic cell (DC) activation and T cell activation in vitro.

Carbon storage regulator CsrA plays important roles in multiple virulence-associated processes of Clostridium difficile.

The carbon storage regulator CsrA is a global regulator that controls multiple virulence-associated processes including host cell invasion, virulence secretion, quorum sensing, biofilm formation, and motility in many pathogenic bacteria. However, the roles of CsrA in Clostridium difficile still remain unclear. In this study, a C.