Publications by authors named "Haolun Chen"
Article Synopsis
- Microglia, immune cells in the brain, become activated during ischemic stroke, leading to further brain damage due to neuroinflammation and cell death (apoptosis).
- Scutellarin shows potential therapeutic effects against ischemic stroke by promoting recovery and reducing harmful protein expressions in microglial cells, as demonstrated in both in vitro and in vivo models.
- The beneficial effects of scutellarin are linked to its ability to modulate the PI3K/AKT/GSK3/NF-κB signaling pathway, which plays a crucial role in controlling inflammation and cell survival.
Previous studies have shown that scutellarin inhibits the excessive activation of microglia, reduces neuronal apoptosis, and exerts neuroprotective effects. However, whether scutellarin regulates activated microglia-mediated neuronal apoptosis and its mechanisms remains unclear. This study aimed to investigate whether scutellarin can attenuate PC12 cell apoptosis induced by activated microglia via the JAK2/STAT3 signalling pathway.
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- Scutellarin is investigated for its potential to treat brain ischaemia, with researchers aiming to uncover its mechanism of action using network pharmacology and lab experiments.
- The study identified and confirmed that the JAK2/STAT3 signaling pathway plays a crucial role in how scutellarin alleviates brain damage during ischaemia.
- Experiments showed that scutellarin significantly increased the levels of JAK2 and STAT3 proteins in astrocytes, supporting its therapeutic effects and providing a foundation for clinical use.
Scutellarin, an herbal agent, is known to possess anti-oxidant and anti-inflammatory properties. In activated microglia, it has been reported that this is achieved through acting on the MAPKs, a key pathway that regulates microglia activation. This study sought to determine if scutellarin would affect the commonly described microglia phenotypes, namely, M1 and M2, thought to contribute to pro- and anti-inflammatory roles, respectively.
View Article and Find Full Text PDFBackground: Scutellarin, an herbal compound, can effectively suppress the inflammatory response in activated microglia/brain macrophage(AM/BM) in experimentally induced cerebral ischemia; however, the underlying mechanism for this has not been fully clarified. We sought to elucidate if scutellarin would exert its anti-inflammatory effects on AM/BM through the MAPKs pathway.
Materials And Methods: Western blot and immunofluorescence labeling were used to determine the expression of the MAPKs pathway in AM/BM in rats subjected to middle cerebral artery occlusion (MCAO) also in lipopolysaccharide (LPS)-activated BV-2 microglia in vitro.
An actinomycete strain TRM20105 with antifungal activity was identified as subsp. DSM43111 (98.34% similarity) by 16S rDNA phylogenetic analysis and morphology observation.
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