Publications by authors named "Haoju Hua"
Article Synopsis
- Optimizing the druggability of bioactive proteins is essential to enhance their therapeutic effects, focusing on improving pharmacokinetic properties without losing biological activity.* -
- The study introduces a new platform called lipidation-dimerization (LiDi) that combines lipidation and a dimeric form of Fibroblast growth factor 21 (FGF21), a hormone with potential for treating non-alcoholic steatohepatitis (NASH).* -
- In vivo tests show that the LiDi FGF21 analogs provide better pharmacokinetic properties and efficacy for NASH compared to existing treatments, effectively prolonging therapeutic effects while maintaining activity.*
Article Synopsis
- AA139 is a potent antimicrobial peptide with strong activity against multidrug-resistant and extensively drug-resistant Gram-negative bacteria, but current production yields are low and chemical synthesis is costly.* -
- The study reveals a new bioprocess for producing AA139 using E. coli with SUMO fusion technology, leading to a more efficient and simplified production method.* -
- By optimizing high cell density fermentation, researchers achieved a yield of 56 mg/L of rAA139 with 98% purity, marking the highest yield for this peptide reported so far.*
Bulevirtide, an entry inhibitor for the hepatitis B virus (HBV) and hepatitis D virus (HDV), is currently available on the European market. However, its clinical application is constrained by its short half-life and poor water solubility, rendering it unsuitable for fatty acid modification, aimed at achieving long-term effects. To address this limitation, we integrated a polypeptide chain consisting of Pro, Ala, and Ser at the C-terminus, which increased its hydrophilicity.
View Article and Find Full Text PDFAs an excellent fusion tag for expressing heterologous proteins, yeast SUMO (small ubiquitin-related modifier) has unique advantages such as improving solubility, promoting stability, and reducing degradation, but it lacks a simple and rapid purification method. Camelid single-domain antibodies (VHHs or nanobodies) show great promise as an efficient tool in analytical application. In this study, VHHs against SUMO protein were isolated for the first time using biopanning of an immune camelid nanobody library.
View Article and Find Full Text PDFCloning, expression, and purification of porcine adrenocorticotropic hormone in Escherichia coli.
Protein Expr Purif
December 2020
Adrenocorticotropic hormone (ACTH) is an old medicine derived from porcine pituitary gland that has been marketed for more than 60 years. In this study, we present a recombinant approach to produce ACTH in Escherichia coli (E. coli).
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