Publications by authors named "Haojin Gao"

Article Synopsis
  • The study investigates the mechanisms of resistance to ceftazidime-avibactam (CZA) in a hypervirulent strain of Klebsiella pneumoniae known as CRE146, which carries the bla gene.
  • Researchers isolated twelve carbapenem-resistant Klebsiella pneumoniae strains from a single patient, employing whole genome sequencing and various pathogenicity tests to understand the virulence factors and resistance development.
  • Findings revealed that the resistance was linked to drug exposure and involved specific genetic mutations in carbapenemase (KPC-228), with CRE146 showing high resistance to CZA while being more susceptible to other antibiotics like meropenem and imipenem.
View Article and Find Full Text PDF
Article Synopsis
  • - The study investigates two types of Klebsiella pneumoniae: carbapenem-resistant hypervirulent (CR-hvKP) and hypervirulent carbapenem-resistant (hv-CRKP), focusing on their antibiotic resistance and virulence profiles.
  • - Researchers identified these strains through whole-genome analysis and several virulence tests, finding that 17.4% were CR-hvKP and 82.6% were hv-CRKP, with CR-hvKP displaying lower resistance to certain antibiotics.
  • - The analysis revealed that while CR-hvKP and hv-CRKP had similar virulence potential, a predominant subclone ST11-KL64 emerged with high resistance and virulence, suggesting an evolving threat
View Article and Find Full Text PDF

Plasmid conjugation is a central mechanism driving the dissemination of antibiotic resistance in Klebsiella pneumoniae. However, the conjugative operon requires specific stimuli for activation. Identifying signals and elucidating the underlying mechanisms is crucial in combating plasmid spread.

View Article and Find Full Text PDF

The ability to form robust biofilms and secrete a diverse array of virulence factors are key pathogenic determinants of , causing a wide range of infectious diseases. Here, we characterized as a VraR-regulated gene encoding a cell wall inhibition-responsive protein (CwrA) using electrophoretic mobility shift assays. We constructed deletion mutants in the genetic background of methicillin-resistant (MRSA) and methicillin-sensitive (MSSA) strains.

View Article and Find Full Text PDF

Unlabelled: The widespread prevalence and dissemination of antibiotic-resistant bacteria, coupled with the diminishing supply of new antibiotics, emphasize the pressing necessity for the exploration of innovative antibacterial agents. Previously, we detailed the impact of the small-molecule compound CY-158-11 on biofilm. By hindering adhesion and PIA-mediated biofilm formation, subinhibitory concentrations of CY-158-11 exhibit antibiofilm activity toward .

View Article and Find Full Text PDF

RNA splicing is a dynamic molecular process in response to environmental stimuli and is strictly regulated by the spliceosome. Sm proteins, constituents of the spliceosome, are key components that mediate splicing reactions; however, their potential role in hepatocellular carcinoma (HCC) is poorly understood. In the study, SNRPD2 (PD2) is found to be the most highly upregulated Sm protein in HCC and to act as an oncogene.

View Article and Find Full Text PDF

Background: Carbapenem-resistant (CRKP) infections are a great threat to public health worldwide. Ceftazidime-avibactam (CZA) is an effective -lactam/-lactamase inhibitors against CRKP. However, reports of resistance to CZA, mainly caused by carbapenemase (KPC) variants, have increased in recent years.

View Article and Find Full Text PDF

B-cell CLL/lymphoma 9 (BCL9) is considered a key developmental regulator and a well-established oncogenic driver in multiple cancer types, mainly through potentiating the Wnt/β-catenin signaling. However, increasing evidences indicate that BCL9 also plays multiple Wnt-independent roles. Herein, we summarized the updates of the canonical and non-canonical functions of BCL9 in cellular, physiological, or pathological processes.

View Article and Find Full Text PDF

Metabolic reprogramming of cancer cells is a hallmark of cancer, in which the polar metabolites involving aerobic glycolysis, pentose phosphate pathway (PPP), tricarboxylic acid (TCA) cycle, and glutaminolysis play a crucial role in the occurrence and development of cancer. Therefore, targeted analysis of the polar metabolites in these pathways is of great value for understanding cancers, finding diagnostic biomarkers, and identifying therapeutic targets. However, it is still challenging to directly determine polar metabolites in these pathways without derivatization due to their diverse chemical properties, isomers, and strong polarity.

View Article and Find Full Text PDF

Bacteria are important symbionts for humans, which sustain substantial influences on our health. Interestingly, some bastrains have been identified to have therapeutic applications, notably for antitumor activity. Thereby, oncologists have developed various therapeutic models and investigated the potential antitumor mechanisms for bacteria-mediated cancer therapy (BCT).

View Article and Find Full Text PDF

Deregulation of alternative splicing is implicated as a relevant source of molecular heterogeneity in cancer. However, the targets and intrinsic mechanisms of splicing in hepatocarcinogenesis are largely unknown. Here, we report a functional impact of a Splicing Regulatory Glutamine/Lysine-Rich Protein 1 (SREK1) variant and its regulator, Serine/arginine-rich splicing factor 10 (SRSF10).

View Article and Find Full Text PDF