Aggregation-Induced Emission Properties of Atypical Aliphatic-Chain-Linked Siloxanes-Containing Phosphonate Esters.

Phosphonate compounds have been proposed as atypical chromophores, but their luminescence properties, especially in combination with flexible aliphatic chains, remain underexplored. In this study, we have synthesized a series of novel siloxane-containing phosphonate esters as organofluorophores through a catalyst-free, one-pot Kabachnik-Fields (K-F) reaction. This reaction involved acetone, cyclohexanone, or cyclopentanone, with siloxanes containing aliphatic amines and phosphonate diesters as reactants.

PM-profiler: a high-resolution and fast tool for taxonomy annotation of amplicon-based microbiome.

Amplicon sequencing stands as a cornerstone in microbiome profiling, yet concerns persist regarding its resolution and accuracy. The enhancement of reference databases and annotations marks a new era for 16S rRNA-based profiling. Capitalizing on this potential, we introduce PM-profiler, a novel tool for profiling amplicon short reads.

Effects of meteorological factors and groundwater depths on sap flow density of in a desert oasis, Taklamakan Desert, China.

Accurate estimation of desert vegetation transpiration is key to regulating desert water resources of desert ecosystems. Sap flow density (SFD) can indirectly reflect a tree's transpiration consumption, and it has been affected by climate warming and groundwater depths in desert ecosystems. Sap flow responses to meteorological conditions and groundwater depths are further affected by tree of different sizes.

Comprehensive Assessment of 16S rRNA Gene Amplicon Sequencing for Microbiome Profiling across Multiple Habitats.

The 16S rRNA gene works as a rapid and effective marker for the identification of microorganisms in complex communities; hence, a huge number of microbiomes have been surveyed by 16S amplicon-based sequencing. The resolution of the 16S rRNA gene is always considered only at the genus level; however, it has not been verified on a wide range of microbes yet. To fully explore the ability and potential of the 16S rRNA gene in microbial profiling, here, we propose Qscore, a comprehensive method to evaluate the performance of amplicons by integrating the amplification rate, multitier taxonomic annotation, sequence type, and length.

The combined effect of surface water and groundwater on environmental heterogeneity reveals the basis of beta diversity pattern in desert oasis communities.

Beta diversity indicates the species turnover with respect to a particular environmental gradient. It is crucial for understanding biodiversity maintenance mechanisms and for prescribing conservation measures. In this study, we aimed to reveal the drivers of beta diversity patterns in desert hinterland oasis communities by establishing three types of surface water disturbance and groundwater depth gradients.

A Potent Micron Neoantigen Tumor Vaccine GP-Neoantigen Induces Robust Antitumor Activity in Multiple Tumor Models.

Therapeutic tumor neoantigen vaccines have been widely studied given their good safety profile and ability to avoid central thymic tolerance. However, targeting antigen-presenting cells (APCs) and inducing robust neoantigen-specific cellular immunity remain challenges. Here, a safe and broad-spectrum neoantigen vaccine delivery system is proposed (GP-Neoantigen) based on β-1,3-glucan particles (GPs) derived from Saccharomyces cerevisiae and coupling peptide antigens with GPs through convenient click chemistry.

P22 virus-like particles as an effective antigen delivery nanoplatform for cancer immunotherapy.

As a new strategy for cancer immunotherapy, therapeutic cancer vaccines have been greatly improved in recent years. However, addressing the needs to quickly and efficiently elicit a high-intensity immune response against neoantigen peptides, especially to induce an effective cytotoxic lymphocyte (CTL) reaction, remain challenges in this field. In this study, virus-like particles (VLPs) derived from the phage P22 were adopted to load peptide antigens on the surface, to test whether VLP technology can be used as a platform for efficient peptide antigen delivery by therapeutic cancer vaccines.

PEG Linker Improves Antitumor Efficacy and Safety of Affibody-Based Drug Conjugates.

Antibody drug conjugates (ADCs) have become an important modality of clinical cancer treatment. However, traditional ADCs have some limitations, such as reduced permeability in solid tumors due to the high molecular weight of monoclonal antibodies, difficulty in preparation and heterogeneity of products due to the high drug/antibody ratio (4-8 small molecules per antibody). Miniaturized ADCs may be a potential solution, although their short circulation half-life may lead to new problems.