Regulating the nitrogen content in graphene to modify manganese dioxide for the sensitive detection of dopamine in biological samples.

A 3D neural network-shaped nitrogen-doped graphene-coated MnO composite (N@Gr-MnO) was successfully prepared by hydrothermal method. The nitrogen content in graphene is effectively regulated by changing the amount of urea added. Upon achieving a graphene oxide (GO) to urea mass proportion of 1/200, the resultant N@Gr exhibited maximal nitrogen concentration.

Simultaneous optimization of conductive layer, dielectric layer, and fabrication process for optically transparent low-loss frequency selective surfaces.

This work presents a design approach for optically transparent low-loss frequency selective surfaces (FSSs) based on the simultaneous optimization of a conductive layer, dielectric layer, and fabrication process. Three bandpass FSSs working in millimeter-wave bands with low insertion losses are investigated and fabricated based on low-loss Rogers RT5870 and cyclic olefin copolymer (COC) substrates. The measured results of the RT5870 based FSS (case 1) and COC based FSS (case 2) indicate that the COC substrate possesses lower dielectric loss tangent at the K band.

Facilitation of coke gasification by coking wastewater and its degradation properties in thermochemical reactions.

The thermochemical reaction between coking wastewater and gasification-coke is a new way to achieve efficient and clean use of coal and solve the problem of coking wastewater treatment in the Gansu Longdong region. To further investigate the thermochemical reaction characteristics of coking wastewater and gasification-coke, this paper explores the effect of coking wastewater on syngas production from coke gasification at 1000 °C, the degradation effect of organic pollutants in coking wastewater, the thermochemical reactivity of gasification-coke, and the reasons why wastewater promotes coke gasification and wastewater degradation. The results showed that the thermochemical reaction of coking wastewater with gasification-coke not only facilitates the improvement of syngas yield and low-level calorific value but also improves the thermochemical reactivity of gasification-coke.

One-pot synthesis and hydrogen peroxide electrochemical sensing of 3D TiO/MnO nanorods assembled microspheres.

Nanorods assembled 3D microspheres of TiO/MnO were prepared via a simple one-pot hydrothermal approach. The resultant composite material exhibited remarkable electrocatalytic activity for hydrogen peroxide (HO) in comparison to each single component. The electrochemical sensor constructed with TiO/MnO exhibited a linear relationship within the range 0.

Misdiagnosis of lymphoma as vasculitis: A case report.

NK/T-cell lymphoma (NKTCL) is a highly aggressive malignant tumour with a very poor prognosis, which often poses diagnostic difficulties due to the non-specificity of its clinical presentation. NK/T-cell lymphoma with eosinophilic hyperplasia syndrome is extremely rare. This article describes a patient with NKTCL misdiagnosed as vasculitis who presented with sinusitis, abdominal pain, anorexia, and lung shadows.

Pillar[5]arene-based supramolecular pseudorotaxane polymer material for ultra-sensitive detection of Fe and F.

Recent advancements in ultra-sensitive detection, particularly the Aggregation Induced Emission (AIE) materials, have demonstrated a promising detection method due to their low cost, real-time detection, and simplicity of operation. Here, coumarin functionalized pillar[5]arene (P5C) and bis-bromohexyl pillar[5]arene (DP5) were successfully combined to create a linear AIE supramolecular pseudorotaxane polymer (PCDP-G). The use of PCDP-G as a supramolecular AIE polymer material for recyclable ultra-sensitive Fe and F detection is an interesting application of the materials.

Cross-Linked Networks of 1,6-Hexanedithiol with Gold Nanoparticles to Improve Permeation Flux of Polyethersulfone Membrane.

It is a great challenge to design and prepare polymeric membranes with excellent permeability and good rejection. In this study, a modifier of gold nanoparticles for crosslinking and self-assembly by 1,6-hexanedithiol is fabricated and used to modify the polyethersulfone membrane as an additive, which forms a uniform porous membrane by liquid-liquid phase conversion technology. The morphology of the membrane is investigated by scanning electron microscopy (SEM), the change of the hydrophilicity of the membrane surface after modification is measured by the contact angle goniometer, and the performance of the fabricated membrane is measured by evaluating the pure water flux and the rejection ratio of bovine serum albumin.

Matrix Solid-Phase Dispersion Coupled with HPLC-UV for Simultaneous Extraction, Purification and Determination of Six Lignans in Schisandra chinensis Fruits.

An efficient method for simultaneous extraction, purification and determination of six lignans in Schisandra chinensis Baill was developed by employing matrix solid-phase dispersion (MSPD) extraction followed by HPLC-UV determination analysis. Several sorbent and desorption solvent that affected the extraction yield of lignans were investigated; neutral alumina and absolute ethanol were selected as the best dispersing material and desorption agent, respectively. Other extraction conditions for MSPD were optimized as follows: 1:2 of S.

Ultrasound-Assisted Extraction of Water-Soluble Polysaccharides from the Fruit of Acanthopanaxbrachypus: Physicochemical, Structural and Functional Properties.

The ultrasound-assisted extraction (UAE) parameters of total water-soluble polysaccharides (TABPs) from Acanthopanaxbrachypus fruit were optimized by Box-Behnken design (BBD) and response surface methodology (RSM). Physicochemical, structural, and functional properties of TABPs were investigated by chemical analysis, inductively coupled plasma optical emission spectrometry (ICP-OES), high performance liquid chromatography (HPLC), scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), thermogravimetric analysis (TGA), water-holding capacity (WHC), oil-holding capacity (OHC), emulsion capacity (EC), emulsion stability (ES), as well as DPPH and ABTS scavenging assays. The results showed that the maximal UAE-yield of TABPs was 3.

Chemical modification and antioxidant activity of the polysaccharide from Acanthopanax leucorrhizus.

A water-soluble polysaccharide (ALP) from Acanthopanax leucorrhizus was chemically modified to obtain two sulfated derivatives (S-ALP1 and S-ALP2), two phosphorylated derivatives (P-ALP1 and P-ALP2) and two acetylated derivatives (A-ALP1 and A-ALP2) with different degree of substitution (DS). Their structures were characterized by chemical and spectral (IR, C NMR and P NMR) analysis, and in vitro antioxidant activity were evaluated by reducing power assay, DPPH• and HO• scavenging assay. The results obtained showed that the modifications were successful, although the molecular weight (Mw) of the ALP derivatives decreased due to slight degradation during the reaction.

Isolation, purification, characterization and antioxidant activity of polysaccharides from the stem barks of Acanthopanax leucorrhizus.

A novel water-soluble polysaccharide (named ALP-1) was successfully isolated from the stem barks of Acanthopanax leucorrhizus by hot-water extraction, and further purified by Cellulose DEAE-52 and Sephadex G-100 chromatography. The structure of ALP-1 was characterized by HPLC, HPGPC, partial acid hydrolysis, periodate oxidation, Smith degradation, methylation, together with UV, IR and NMR spectral analysis. The antioxidant activities also were evaluated in vitro.

Signalling mechanism(s) of epithelial-mesenchymal transition and cancer stem cells in tumour therapeutic resistance.

Unlabelled: Epithelial-mesenchymal transition (EMT) leads to tumour progression, including tumour metastasis, disease recurrence and therapy resistance. Cancer stem cells (CSCs) are a small group of cells that have the ability to undergo self-renewal and heterogeneous differentiation, which play a key role in the occurrence and development of cancer. EMT can promote tumour cells to develop stem cell characteristics, which makes tumours more difficult to treat.

Isolation, modification and cytotoxic evaluation of stilbenoids from Acanthopanax leucorrhizus.

Twenty natural stilbenoids (1-20), including seven new stilbenoids (2, 4-7, 19, 20) and thirteen known stilbenoids (1, 3, 8-18), were isolated from the stem barks of Acanthopanax leucorrhizus, and six modified stilbenoid derivatives (1a, 2a, 4a, 4b, 7a and 17a) were obtained via methylation, demethylation and isopentenylation of the corresponding isolates (1, 2, 4, 7 and 17). These stilbenoids were structurally characterized by comprehensive analysis of their spectroscopic data and comparison with literature information, and evaluated for their cytotoxic activities against three human tumor cell lines (leukemia HL-60, hepatoma SMMC-7721 and breast carcinoma MCF-7) in vitro by MTT assay. The results showed that compounds 1a, 4a and 4b showed potent selective cytotoxicity against SMMC-7721 (IC=10.

Structural Modification of Stilbenoids from Acanthopanax leucorrhizus and Their Cytotoxic Activity.

A new cis-stilbenoid, 1,9-dihydroxy-10-methoxy-6H-dibenzo[b,f]oxocin-6-one (2) was isolated from the AcOEt extract of the stem barks of Acanthopanax leucorrhizus, along with three known stilbenoids, 9-hydroxy-10-methoxy-6H-dibenzo[b,f]oxocin-6-one (1), 5-O-methyl-(E)-resveratrol 3-O-β-d-glucopyranoside (3), and (E)-resveratrol 3-O-β-d-xylopyranoside (4). Two derivatives (2a and 2b) were synthesized by the structural modification of compound 2, which exhibited certain cytotoxic activities against HT-29 and HeLa cell lines in vitro. All compounds were structurally characterized by comprehensive analysis of their spectroscopic data and comparison with literature information, and evaluated for their cytotoxic activities against three human tumor cell lines (HL-60, HT-29, and HeLa) by the standard MTT assay in vitro.

GKN2 increases apoptosis, reduces the proliferation and invasion ability of gastric cancer cells through down-regulating the JAK/STAT signaling pathway.

Objectives: To investigate the effect of gastric motility protein 2 (GKN2) on the proliferation, apoptosis and invasion of gastric cancer cell and on the JAK/signal transducer and activator of transcription 3 (STAT3) signaling pathway.

Methods: Expression of GKN2 was qualified using Western blot analysis in four gastric cancer cell lines and immortalized human gastric mucosal epithelial cell line GES-1. The cells were then transfected with pcDNA3.

[Change of hepatic drug metabolism enzymes in rat depression model with kidney-yang deficiency].

This study was designed to explore the impact of depression on kidney-yang deficiency in rats. Rats were repeatedly injected with hydrocortisone for 21 days to establish the depression model with kidneyyang deficiency. Tolbutamide, chlorzoxazone, theophylline, midazolam, omeprazole and dextromethorphan were used as substrates of CYP2C6, CYP2E1, CYP1A2, CYP3A2, CYP2D1, and CYP2D2 to test the depression impact on drug metabolism.

Identification of potential targets for differentiation in human leukemia cells induced by diallyl disulfide.

Diallyl disulfide (DADS) is a primary component of garlic, which has chemopreventive potential. We previously found that moderate doses (15-120 µM) of DADS induced apoptosis and G2/M phase cell cycle arrest. In this study, we observed the effect of low doses (8 µM) of DADS on human leukemia HL-60 cells.

Extraction optimization, characterization and antioxidant activity of polysaccharide from Gentiana scabra bge.

In this study, optimization of smashing tissue extraction (STE), preliminary chemical characterization and antioxidant activity in vitro of crude polysaccharides (CPS) from Gentiana scabra bge (G. scabra) were investigated. The optimal extraction conditions were determined as follows: sample particle size of 80mesh, solid/liquid ratio of 1:34, extraction voltage of 157.

[Animal drugs quality status and reason analysis].

In order to reaction the quality present situation, problems on the current quality of animal sources of drugs are summed up by using test data analysis, literature search and marketing research. This paper can also help the improvement of the quality management, the revision of the relevant department policy system and the improvement of standards.

Trefoil factors: Gastrointestinal-specific proteins associated with gastric cancer.

Trefoil factor family (TFF), composed of TFF1, TFF2, and TFF3, is a cluster of secreted peptides characterized by trefoil domain (s) and C-terminal dimerization domain. TFF1, a gastric tumor suppressor, is a single trefoil peptide originally detected in breast cancer cell lines but expressed mainly in the stomach; TFF2, a candidate of gastric cancer suppressor with two trefoil domains, is abundant in the stomach and duodenal Brunner's glands; and TFF3 is another single trefoil peptide expressed throughout the intestine which can promote the development of gastric carcinoma. According to multiple studies, TFFs play a regulatory function in the mammals' digestive system, namely in mucosal protection and epithelial cell reconstruction, tumor suppression or promotion, signal transduction and the regulation of proliferation and apoptosis.

Isolation, purification and structural characterization of polysaccharide from Acanthopanax brachypus.

A water-soluble polysaccharide, designated as ABPS-21, was isolated from the stem barks of Acanthopanax brachypus by hot-water extraction and purified by Cellulose DEAE-52 and Sephacryl S-300 HR gel-filtration chromatography, respectively. The homogeneity and molecular weight were determined using HPGPC. The structure was elucidated based on monosaccharide composition and methylation analysis, partial acid hydrolysis, periodate oxidation, Smith degradation, IR and NMR spectroscopy.

Optimization of microwave-assisted enzymatic extraction of polysaccharides from the fruit of Schisandra chinensis Baill.

A microwave-assisted enzymatic extraction (MAEE) method had been developed, which was optimized by response surface methodology (RSM) and orthogonal test design, to enhance the extraction of crude polysaccharides (CPS) from the fruit of Schisandra chinensis Baill. The optimum conditions were as follows: microwave irradiation time of 10 min, extraction pH of 4.21, extraction temperature of 47.

Two-steps extraction of essential oil, polysaccharides and biphenyl cyclooctene lignans from Schisandra chinensis Baill fruits.

A method for two-steps extraction of essential oil, polysaccharides and lignans from Schisandra chinensis Baill had been established. Firstly, S. chinensis was extracted by hydro-distillation, the extracted solution was separated from the water-insoluble residue and precipitated by adding dehydrated alcohol after the essential oil was collected, and then the precipitate as polysaccharide was collected.