Effects of high stocking density on the growth performance, intestinal health and bile salts composition of broiler chickens.

Introduction: The intestinal dysfunction plays an important role in the decreased growth performance of broiler chickens under high stocking density. Gut microbiota plays an important role in maintaining intestinal health. However, the modulation pathway of gut microbiota by regulating the intestinal barrier and histomorphology remains unknown.

Design and development of a novel multi-epitope DNA vaccine candidate against infectious bronchitis virus: an immunoinformatic approach.

Avian infectious bronchitis (IB) is one of the major respiratory diseases in poultry. At present, attenuated vaccines are the main commercial vaccines, but they have many defects. We aimed to construct a novel multi-epitope DNA vaccine based on avian infectious bronchitis virus (IBV) S1 and N proteins for the prevention of IBV infection.

Design, Synthesis, and Biological Evaluation of Marchantin C-NO Donor Hybrids for Overcoming Pgp-Mediated Drug Resistance by Targeting Lysosome.

A series of marchantin C-NO donor hybrids were designed, synthesized, and evaluated for their antitumor activity and . Notably, MC-furoxan hybrid exhibited the best selective inhibitory activity against MCF-7/ADR (IC = 0.024 μM) with 883 times potency compared with MCF-7 cells (IC = 21.

Dual-function regulator MexL as a target to control phenazines production and pathogenesis of Pseudomonas aeruginosa.

Antibiotic resistance or tolerance of pathogens has become one of the global public crises. Finding new drug targets may open up a way of infection control. Phenazine pyocyanin (PYO) is an important virulence factor produced by the pathogen Pseudomonas aeruginosa.

Melatonin Modulates ZAP70 and CD40 Transcripts via Histone Modifications in Canine Ileum Epithelial Cells.

Melatonin (MLT), produced by the pineal gland and other tissues, is known for its anti-inflammatory effects, particularly in regulating inflammatory markers and cytokines in intestinal cells. Our study aimed to investigate how MLT influences the expression of inflammatory genes through histone modification in canine ileum epithelial cells (cIECs). In our experiment, cIECs were cultured and divided into a control group (CON) and an MLT-treatment group.

Discovery of potent quinone oxidoreductase 2 inhibitors to overcome TRAIL resistance of non-small cell lung cancer.

Resistance to tumor-necrosis-factor-related apoptosis-inducing ligand (TRAIL) of cancer cells is a main obstacle for the chemotherapy. NRH: quinone oxidoreductase 2 (NQO2), known as a chemopreventive target, has emerged as a promising therapeutic target for overcoming TRAIL resistance in non-small cell lung cancer (NSCLC). Here we report the design, synthesis and evaluation of resveratrol analogues as novel selective NQO2 inhibitors, and analogue 20b, with potent NQO2 inhibitory activity (IC = 95 nM) and relatively low cytotoxicity, displayed synergistic lethal effects in combination with TRAIL on TRAIL-resistant NSCLC cells.

Ion-conductive hydrogel sensor prepared with alginate crosslinker for wide-range motion and temperature monitoring.

Flexible wearable electronic devices have gained widespread attention due to their comfort and real-time information transmission capabilities. However, they often exhibit poor stretchability, low sensitivity, and limited functionality, which restricts their practical applications. Herein, a conductive hydrogel (PNAL, a polymer synthesized from polyvinyl alcohol, nipaam, acrylic acid, and lithium chloride) that responds to both strain and temperature simultaneously was developed using a natural polysaccharide composite (SA-AGE) synthesized from sodium alginate (SA) and allyl glycidyl ether (AGE) as a crosslinking agent.

Impact of radiation dose to immune cells on survival in patients with esophageal cancer receiving neoadjuvant chemoradiotherapy: a retrospective analysis.

Background: Neoadjuvant chemoradiotherapy (NCRT) is the standard treatment for locally advanced esophageal squamous cell carcinoma (ESCC). This study aims to explore the effect of the estimated dose of radiation to immune cells (EDRIC) on lymphocytes in patients with locally advanced ESCC undergoing NCRT and evaluate the impact of EDRIC on patient prognosis.

Methods: This retrospective study included patients with locally advanced ESCC who received NCRT followed by radical esophagectomy at our institution between March 2016 and December 2022.

Single-cell transcriptome-wide Mendelian randomization and colocalization reveals immune-mediated regulatory mechanisms and drug targets for COVID-19.

Background: COVID-19 continues to show long-term impacts on our health. Limited effective immune-mediated antiviral drugs have been launched.

Methods: We conducted a Mendelian randomization (MR) and colocalization analysis using 26,597 single-cell expression quantitative trait loci (sc-eQTL) to proxy effects of expressions of 16,597 genes in 14 peripheral blood immune cells and tested them against four COVID-19 outcomes from COVID-19 Genetic Housing Initiative GWAS meta-analysis Round 7.

First discovery of the genus Stephens (Coleoptera, Dasytidae) from China.

Background: Stephens, 1830, is classified within the subfamily Dasytinae of the family Dasytidae (Coleoptera, Cleroidea). Currently, it comprises two subgenera and 27 known species. This genus primarily inhabits south-central Europe, with certain species extending into west Asia; however, it has not been reported in China until now.

Dendrite formation in solid-state batteries arising from lithium plating and electrolyte reduction.

All-solid-state batteries offer high-energy-density and eco-friendly energy storage but face commercial hurdles due to dendrite formation, especially with lithium metal anodes. Here we report that dendrite formation in Li/LiLaZrO/Li batteries occurs via two distinct mechanisms, using non-invasive solid-state nuclear magnetic resonance and magnetic resonance imaging. Tracer-exchange nuclear magnetic resonance shows non-uniform Li plating at electrode-electrolyte interfaces and local Li reduction at LiLaZrO grain boundaries.

Fungi in the Gut Microbiota: Interactions, Homeostasis, and Host Physiology.

The mammalian gastrointestinal tract is a stage for dynamic inter-kingdom interactions among bacteria, fungi, viruses, and protozoa, which collectively shape the gut micro-ecology and influence host physiology. Despite being a modest fraction, the fungal community, also referred to as mycobiota, represents a critical component of the gut microbiota. Emerging evidence suggests that fungi act as early colonizers of the intestine, exerting a lasting influence on gut development.

First record of the genus Tshernyshev, 2009 (Coleoptera, Malachiidae) from China.

Background: Tshernyshev, 2009 is a small genus belonging to the tribe Malachiini in the family Malachiidae of the order Coleoptera, with six currently known species: (Erichson, 1840) from Central, Eastern and Southern Europe and North Africa; (Abeille de Perrin, 1885) from Central Europe to Central Asia; Ezzatpanah, 2011 from Iran; (Tshernyshev, 2000) from Uzbekistan and Kyrgyzstan; (Medvedev, 1964) from Kazakstan, Russia and Mongolia; (Medvedev, 1964) from Kazakhstan and Turkey.

New Information: In this study, Tshernyshev, 2009 is newly recorded from China upon the discovery of (Medvedev, 1964) from Xinjiang Autonomous Region. The adult morphological characters are re-described in detail with illustrations of external appearance and relevant characters.

[A comparative study of dynamic versus static rehabilitation protocols after acute Achilles tendon rupture repair with channel assisted minimally invasive repair technique].

Objective: To explore the difference in effectiveness between a dynamic rehabilitation protocol and a traditional static rehabilitation protocol after the treatment of acute Achilles tendon rupture with channel assisted minimally invasive repair (CAMIR) technique through a prospective comparative trial, aiming to provide a reference for clinically selecting a feasible treatment regimen.

Methods: Patients with acute Achilles tendon rupture admitted between June 2021 and June 2022 were included in the study, with 60 patients meeting the selection criteria. They were randomly divided into a dynamic rehabilitation group ( =30) and a static rehabilitation group ( =30) using a computer-generated random number method.

A review of Plateros Bourgeois, 1879 (Coleoptera: Lycidae) from southeastern China, with descriptions of six new species from the Nanling Mountains.

We review the species of the lycid genus Plateros Bourgeois, 1879 from Southeast China in this study. Six new species are described, including P. flavihumeralis sp.

Four new species of the Mesolycus ilyai species group (Coleoptera: Lycidae) from China.

Four new species of the Mesolycus ilyai species group are described from China, namely, M. baoi sp. nov.

Establishment of two species groups of Ichthyurus Westwood (Coleoptera: Cantharidae), with description of two new species from China and Vietnam.

Two species groups of the genus Ichthyurus Westwood, 1848 in the Oriental Region are proposed based on the anatomical comparison. One is the I. vandepolli species group, which is composed of two species, namely I.

A multi-levels analysis to evaluate the toxicity of microplastics on aquatic insects: A case study with damselfly larvae (Ischnura elegans).

Microplastic (MP) pollution prevalent in freshwater environments and jeopardizes the organisms living there. Dozens of studies have been conducted to investigate the harmful effects of microplastics on organisms. However, the most diverse and sensitive aquatic insects are often overlooked, also there is a lack of a comprehensive research exploring the toxicity of microplastics.

[Anatomical research of positional relationship between protective channel and sural nerve during Achilles tendon repair using channel assisted minimally invasive repair technique].

Objective: To evaluate the positional relationship between protective channel and sural nerve while treating acute Achilles tendon rupture with channel assisted minimally invasive repair (CAMIR) technique based on anatomical observations of cadaver specimens.

Methods: Twelve adult cadaveric lower limb specimens (6 left, 6 right) were utilized. A CAMIR device was implanted at a distance of 4 cm from the proximal end of the specimen to the Achilles tendon insertion.