[Chemical Constituents from Thalictrum fortune].

Objective: To investigate the chemical constituents of Thalictrum fortunei.

Methods: Compounds were separated and purified by chromatographic methods and the structures were identified by their physicochemical properties and spectroscopic data.

Results: Ten compounds were isolated and identified as bergenin( 1),1-( 4-hydroxy-3-methoxy)-phenyl-2-[4-( 1,2,3-trihydroxypropyl)-2-methoxy]-phenoxym-1,3-propandiol( 2) 、4-( 2-hydroxyethyl)-2-methoxyphenyl-O-β-D-glucopyranoside( 3),meliasendanin D( 4),2-( 4-hydroxy-3-methoxyphenyl)-ethyl-O-β-D-glucopyranoside( 5),kizutasaponin C( 6),2-( 3-hydroxy-4-methoxyphenyl)-ethyl-O-β-Dglucopyranoside( 7),β-sitosterol( 8),3-O-β-D-glucopyranosyl( 1→6)-β-D-glucopyranosyl( 22 S,24Z)-cycloart-24-en-3β,22,30-tetraol-26-O-β-D-glucopyranoside( 9) and 3-O-β-D-quinovopyranosyl( 1→6)-β-D-glucopyranosyl( 1→4)-β-D-fucopyranosyl( 22 S,24Z)-cycloart-24-en-3β,22,26-triaol-26-O-β-D-glucopyranoside( 10).

Therapeutic effects of naringin in a guinea pig model of ovalbumin-induced cough-variant asthma.

Naringin, a well known component isolated from Exocarpium Citri Grandis, has significant antitussive effects. Recently, Naringin exhibited novel anti-inflammatory effect in chronic inflammatory diseases. In this work, we firstly evaluated the effects of naringin on enhanced cough, airway hyper-responsiveness (AHR), and airway inflammation in an ovalbumin-induced experimental cough-variant asthma (CVA) model in guinea pigs.

[GC-MS study on fingerprint of volatile oil from Kadsura heteroclita].

Objective: To study the fingerprint of volatile oil from Kadsura heteroclita by GC-MS.

Methods: 10 batches of Kadsura heteroclita were analyzed by GC-MS. TIC profiles were evaluated by" computer aided similarity evaluation system".

[Study on pharmacokinetics of ginkgolide B injection in Beagle dogs].

Objective: To study the pharmacokinetics of ginkgolide B injection in Beagle dogs.

Methods: Determined the serum concentration of ginkgolide B by LC-MS and calculated its parameter of pharmacokinetics via DAS 2.0 software.

[Study on the pharmacokinetics of ginkgolide B for injection in rats].

Objective: To study the pharmacokinetics of ginkgolide B for injection in rats.

Methods: The serum concentration of ginkgolide B was determined by LC-MS and calculate its parameter of pharmacokinetics via DAS2.0 software.

Two new saponins from Thalictrum fortunei.

Two new cycloartane glycosides were isolated from the aerial parts of Thalictrum fortunei (Ranunculaceae). The chemical structures of these compounds were elucidated as 3-O-β-D-glucopyranosyl (1 → 4)-β-d-fucopyranosyl-(22S,24Z)-cycloart-24-en-3β,22,26,30-tetraol 26-O-β-D-glucopyranoside and 3-O-β-D-glucopyranosyl (1 → 4)-β-D-fucopyranosyl-(22S,24Z)-cycloart-24-en-3β,22,26,29-tetraol 26-O-β-D-glucopyranoside by extensive 1D and 2D NMR methods, HR-ESI-MS, and hydrolysis. Their cytotoxic activities toward human hepatoma Bel-7402 cells, human colon carcinoma LoVo cells, and human non-small-cell lung cancer NCIH-460 cells were evaluated by MTT assay, respectively.