Eggshell Quality Traits and Transcriptome Gene Screening Between Yunnong and Jingfen Chicken Breeds.

The eggshell quality traits in avian species, including Yunnong chickens (YN), are crucial for commercial and breeding purposes. The use of advanced biotechnologies, such as gene sequencing, has become increasingly common for identifying genetic markers and comparing specific traits in livestock. However, genetic markers related to eggshell traits in YN chickens remain unexplored.

Targeting cholesterol-dependent adrenal steroidogenesis for management of primary aldosteronism.

Primary aldosteronism (PA) is a common, salt-sensitive form of endocrine hypertension. Compared with essential hypertension (EH), PA is more susceptible to cardiorenal complications and metabolic risks. However, PA has a low screening rate and a poor response to mineralocorticoid receptor antagonists (MRAs).

Vertical migration characteristics of polychlorinated biphenyls (PCBs) in estuarine and coastal zones under complex environmental conditions.

As a transitional zone where rivers meet the sea, estuaries are influenced by river transport and ocean tides, resulting in complex variations in parameters such as organic matter content, pH, and sediment salinity. This paper primarily explores the vertical migration patterns of polychlorinated biphenyls (PCBs) under complex conditions, focusing on the soil sediments in the Dagu River estuary area. We designed an indoor soil column leaching experiment to investigate how soil organic matter content, pH, and salinity affect the vertical migration of PCBs in soil.

Effects of Remimazolam on Intraoperative Frontal Alpha Band Power Spectrum Density and Postoperative Cognitive Function in Older Adults Undergoing Lower Extremity Fractures Surgeries: A Randomized Controlled Trial.

Purpose: Low density of electroencephalogram alpha band power was reported to be associated with perioperative cognitive dysfunction. Few studies have conducted to explore the effects of remimazolam on intraoperative frontal alpha band power spectrum density in older adults. Here, we aimed to explore the impact of remimazolam on intraoperative frontal brain wave alpha band activity and postoperative cognitive function in older adults undergoing lower extremity fractures surgeries.

Association between SOFA score and risk of acute kidney injury in patients with diabetic ketoacidosis: an analysis of the MIMIC-IV database.

Introduction: The Sequential Organ Failure Assessment (SOFA) score is a widely utilized clinical tool for evaluating the severity of organ failure in critically ill patients and assessing their condition and prognosis in the intensive care unit (ICU). Research has demonstrated that higher SOFA scores are associated with poorer outcomes in these patients. However, the predictive value of the SOFA score for acute kidney injury (AKI), a common complication of diabetic ketoacidosis (DKA), remains uncertain.

Runx2-NLRP3 Axis Orchestrates Matrix Stiffness-evoked Vascular Smooth Muscle Cell Inflammation.

Arterial stiffening is a hallmark of chronic kidney disease (CKD) related cardiovascular events and is primarily attributed to the elevated matrix stiffness. Stiffened arteries are accompanied by low-grade inflammation, but the causal effects of matrix stiffness on inflammation remain unknown. For analysis of the relationship between arterial stiffness and vascular inflammation, pulse wave velocity (PWV) and aortic inflammatory markers were analyzed in an adenine-induced mouse model of CKD in chronological order.

Knowledge domain and frontier trends of artificial intelligence applied in solid organ transplantation: A visualization analysis.

Background: Solid organ transplantation (SOT) is vital for end-stage organ failure but faces challenges like organ shortage and rejection. Artificial intelligence (AI) offers potential to improve outcomes through better matching, success prediction, and automation. However, the evolution of AI in SOT research remains underexplored.

EIF4A3 Enhances the Proliferation and Cell Cycle Progression of Keloid Fibroblasts by Inducing the hsa_circ_0002198 Expression.

Background: Recent evidence suggests a crucial biological role for Circular RNAs (circRNAs) in keloid diseases, yet the underlying mechanisms remain unclear. This study explored the biological effects and molecular mechanisms of hsa_circ_0002198 in keloid formation.

Methods: Real-time quantitative PCR (qRT-PCR) was employed to assess the expression of circ_0002198 in keloid tissues, normal skin tissues, keloid fibroblasts (KFs), and normal skin fibroblasts (NFs) from nine patients.

CENPE is a diagnostic and prognostic biomarker for cervical cancer.

Cervical squamous cell carcinoma (CESC) is a common cancer in women. Despite advancements in early diagnosis through high-risk human papillomavirus (HPV) screening, challenges remain in predicting and treating the disease. Hence, the identification of novel biomarkers for prognosis and therapeutic targets is crucial.

Olink Profiling of Intestinal Tissue Identifies Novel Biomarkers For Colorectal Cancer.

Comprehensive protein profiling in intestinal tissues provides detailed information about the pathogenesis of colorectal cancer (CRC). This study quantified the expression levels of 92 oncology-related proteins in tumors, paired para-carcinoma tissues, and remote normal tissues from a cohort of 52 CRC patients utilizing the Olink technology. The proteomic profile of normal tissues closely resembled that of para-carcinoma tissues while distinctly differing from that of tumors.

Single-nucleus transcriptome profiling provides insights into the pathophysiology of adhesive arachnoiditis.

Adhesive arachnoiditis (AA) is a rare form of chronic degenerative pathology associated with persistent inflammation in the arachnoid matter of the spinal cord. Despite the existing knowledge, the detailed pathological mechanisms underlying AA are not fully understood. This study aimed to elucidate through comprehensive single nuclei RNA sequencing (snRNA-seq) to delineate the transcriptomic landscape of AA.

Cryo-EM structure and oligomerization of the human planar cell polarity core protein Vangl1.

Vangl is a planar cell polarity (PCP) core protein essential for aligned cell orientation along the epithelial plane perpendicular to the apical-basal direction, which is important for tissue morphogenesis, development and collective cell behavior. Mutations in Vangl are associated with developmental defects, including neural tube defects (NTDs), according to human cohort studies of sporadic and familial cases. The complex mechanisms underlying Vangl-mediated PCP signaling or Vangl-associated human congenital diseases have been hampered by the lack of molecular characterizations of Vangl.

Prediction of the need for surgery in patients with unruptured abdominal aortic aneurysm based on SOFA score.

Objective: This retrospective study aimed to explore the association and clinical value of sequential organ failure assessment (SOFA) score on the predictors of adverse events in patients with unruptured abdominal aortic aneurysms (AAA).

Methods: A total of 322 patients from Medical Information Mart for Intensive Care IV database were enrolled. Logistic regression was conducted to explore the association between SOFA and primary outcome (need for surgery, NFS).

Gene Deficiency of δ Subunit-Containing GABA Receptor in mPFC Lead Learning and Memory Impairment in Mice.

Maintaining GABAergic inhibition within physiological limits in the medial prefrontal cortex (mPFC) is critical for working memory. While synaptic GABAR typically mediate the primary component of mPFC inhibition, the role of extrasynaptic δ-GABAR in working memory remains unclear. To investigate this, we used fiber photometry to examine the effects of δ-GABAR in freely moving mice.

Comparison of a Series of Ga-Labeled DOTA-LLP2A Conjugates for Positron Emission Tomography Imaging of Very Late Antigen-4 in Melanoma.

Melanoma, with its steadily rising global incidence, is characterized by high invasiveness, leading to poor prognosis in advanced stages. There remains an unmet clinical need for the development of radiolabeled PET imaging probes for the early diagnosis of melanoma. Integrin VLA-4, a key factor in melanoma metastasis, presents a promising protein target to address the specificity shortcomings of existing probes in melanoma imaging.

CALB1 and RPL23 Are Essential for Maintaining Oocyte Quality and Function During Aging.

With advancing age, significant changes occur in the female reproductive system, the most notable of which is the decline in oocyte quality, a key factor affecting female fertility. However, the mechanisms underlying oocyte aging remain poorly understood. In this study, we obtained oocytes from aged and young female mice and performed single-cell transcriptome sequencing, comparing our findings with existing proteomic analyses.

Patterns of liver fibrosis evolution in Chinese HIV/HBV co-infected adults following 5-year antiretroviral treatment: a longitudinal study using non-invasive APRI and Fib-4 scores.

The long-term effects of combined antiretroviral therapy (ART) on liver fibrosis patterns in adults living with HIV and chronic hepatitis B virus (HBV) are not well understood. Therefore, this study aimed to investigate the trajectories of liver fibrosis and identify the associations of baseline variables with different patterns of liver fibrosis evolution. A total of 333 individuals with HIV/HBV co-infection and undergoing long-term ART were enrolled in this study.

Determining Rate Coefficients for the 11-Atom Reaction via Ring Polymer Molecular Dynamics Based on a 27-Dimensional Potential Energy Surface: The Reaction between -CHCHOO and HO.

Criegee intermediates (CIs) are potentially significant oxidants and a major source of OH radicals in the troposphere. The -CHCHOO intermediate has been confirmed as a crucial component of CIs in the atmospheric environment. Although previous studies have provided some experimental and theoretical rate constants, inconsistencies among these data remain, and the experimental data do not cover the full range of temperatures present in the troposphere.

The effect of glass container surface silanol density on monoclonal antibody formulation stability after application of mechanical shock.

This study investigates the effect of silanol density on the surface of glass containers on the stability of monoclonal antibody (mAb) formulations subjected to mechanical stress. By calcining Type I glass containers at different temperatures, we altered the concentration of silanols on the glass surface and examined its impact on the stability of protein formulations under mechanical stress. Contact angle measurements and Fourier Transform Infrared (FTIR) spectroscopy indicated that silanol formation influences the hydrophilicity of the surface.

Untargeted metabolomics combined with lipidomics revealed the effects of myocardial infarction and exercise rehabilitation on blood circulation metabolism of patients based on liquid chromatography-mass spectrometry.

Myocardial infarction (MI) is a major cause of death worldwide. Exercise rehabilitation (ER) is a powerful tool to improve life quality and prognosis of MI patients. Herein, we developed an untargeted metabolomics combined with lipidomics method to qualitatively and quantitatively detect metabolites in plasma.

Structural changes of the multifidus in animal models of intervertebral disk degeneration: a systematic review.

Study Design: Low back pain (LBP) is a widespread clinical symptom affecting nearly all age groups and is a leading cause of disability worldwide. Degenerative changes in the spine and paraspinal tissues primarily contribute to the etiology of LBP.

Objectives: We conducted this systematic review of animal models of paraspinal muscle (PSM) degeneration secondary to degenerative intervertebral disc (IVD), providing a comprehensive evaluation of PSM structural changes observed in these models at both macroscopic and microscopic levels.

Correction of severe varus deformity in total knee arthroplasty with the technique of tibial plateau reduction.

Background: The optimal soft tissue release technique for severe varus total knee arthroplasty (TKA) remains controversial. The technique of tibial plateau reduction was suggested for severe varus deformity during TKA. The purpose of this study was to evaluate the clinical and radiological outcomes of the technique of tibial plateau reduction.

A meta-analysis and systematic review of myocardial infarction-induced cardiomyocyte proliferation in adult mouse heart.

Background: The proliferation capacity of adult cardiomyocytes is very limited in the normal adult mammalian heart. Previous studies implied that cardiomyocyte proliferation increases after injury stimulation, but the result is controversial partly due to different methodologies. We aim to evaluate whether myocardial infarction (MI) stimulates cardiomyocyte proliferation in adult mice.

Time trends in malaria incidence from 1992 to 2021 in high-risk regions: an age‑period‑cohort analysis based on the Global Burden of Disease study 2021.

Objectives: Malaria, caused by plasmodium parasites, remains one of the world's most significant infectious diseases due to its high incidence and mortality. This study aims to analyze malaria incidence globally, identify high-risk regions, and examine long-term trends in incidence to provide important evidence for malaria eradication.

Methods: We used data from the Global Burden of Disease Study (GBD) 2021, applying the age-period-cohort model to estimate the effects of age, period and cohort on malaria incidence from 1992 to 2021.