Chemical constitutes from Tuber indicum with immunosuppressive activity uncovered by transcriptome analysis.

Three previously undescribed compounds including a polyketide (1) and two lactams (2 and 3) were obtained from Tuber indicum. The structures of new findings were elucidated by HRESIMS, NMR as well as NMR and ECD calculations. Transcriptome analysis through RNA-seq revealed that compound 2 exhibits immunosuppressive activity.

Detection of the tetrahedral reaction intermediate of the reaction of acetyl chloride with ethanol in microdroplets laser desorption/ionization tandem mass spectrometry.

Understanding the fundamental mechanisms of chemical reactions is of great interest to scientists working in many fields as it enables the rationalization, prediction, and design of reactions. Many chemical processes involve the formation of short-lived reaction intermediates, most of which cannot be isolated and are challenging to detect. One such intermediate is the tetrahedral intermediate often proposed to be generated upon the reactions of acetyl chlorides with simple alcohols an addition/elimination mechanism.

Pretreatment elevated fibrinogen level predicts worse oncologic outcomes in upper tract urothelial carcinoma.

This study aimed to further validate the prognostic role of fibrinogen in upper tract urothelial carcinoma (UTUC) in a large Chinese cohort. A total of 703 patients who underwent radical nephroureterectomy were retrospectively identified. Fibrinogen levels of ≥4.

Polar Effects Control the Gas-Phase Reactivity of para-Benzyne Analogs.

We report herein a gas-phase reactivity study on a para-benzyne cation and its three cyano-substituted, isomeric derivatives performed using a dual-linear quadrupole ion trap mass spectrometer. All four biradicals were found to undergo primary and secondary radical reactions analogous to those observed for the related monoradicals, indicating the presence of two reactive radical sites. The reactivity of all biradicals is substantially lower than that of the related monoradicals, as expected based on the singlet ground states of the biradicals.