Timing of thoracic radiotherapy combined with immunotherapy influences pulmonary injury.

The present study aimed to assess the impact of chest radiotherapy combined with immunotherapy at different time points in lung injury. This retrospective study analyzed 35 patients with thoracic tumors (29 lung cancer cases and 6 esophageal cancer cases) who received radiotherapy combined with immunotherapy between January 2021 and December 2023 at at Capital Medical University, affiliated with Beijing Luhe Hospital (Beijing, China), with a median follow-up time of 21 months. Patients were divided into two groups: Group A (sequential, n=17), who received immunotherapy 2 weeks to 6 months before or after radiotherapy, and group B (synchronous, n=18), who received immunotherapy within 2 weeks before or after radiotherapy.

Exploring druggable targets and inflammation-mediated pathways in cancer: a Mendelian randomization analysis integrating transcriptomic and proteomic data.

Background: Cancer remains a predominant global health challenge, necessitating the ongoing exploration of novel biomarkers and therapeutic targets to improve diagnosis and treatment.

Methods: By integrating expression quantitative trait loci (eQTL) and protein quantitative trait loci (pQTL) data with genome-wide association studies (GWAS) data, we performed Mendelian randomization (MR) analysis to identify potential druggable targets at the gene expression and protein levels for multiple cancers. We conducted mediation analysis to explore whether inflammatory factors mediate the pathways linking identified druggable targets to cancer.

A Mutation in the ANK2 Gene Causing ASD and a Review of the Literature.

Objective: To investigate the clinical and genetic characteristics of patients with ANK2(HGNC:493)-associated autism spectrum disorders (ASDs) and epilepsy (EP).

Methods: We identified a novel ANK2 variant in a patient with ASD and EP and summarized the clinical and genetic characteristics of ANK2 gene variants in this patient and those in previous reports.

Results: A novel nonsense variant, ANK2 (NM_001148.

Advances in porcine epidemic diarrhea virus research: genome, epidemiology, vaccines, and detection methods.

Porcine epidemic diarrhea (PED) is a highly contagious intestinal disease caused by the porcine epidemic diarrhea virus (PEDV). The economic impact of PEDV on the global pig industry has been significant, resulting in considerable losses. This paper presents a review of the latest research progress on PEDV genome, molecular epidemiology, vaccine development, and molecular detection methods.

Contact patterns between index patients and their close contacts and assessing risk for COVID-19 transmission during different exposure time windows: a large retrospective observational study of 450 770 close contacts in Shanghai.

Introduction: To characterise age-mixing patterns among index cases and contacts of COVID-19, and explore when patients are most infectious during the disease process.

Methods: This study examined all initial 90 885 confirmed index cases in Shanghai and their 450 770 close contacts. A generalised additive mixed model was used to analyse the associations of the number of close contacts with different demographic and clinical characteristics.

Colossal Electromechanical Response in Antiferroelectric-based Nanoscale Multilayers.

The pursuit of smaller, energy-efficient devices drives the exploration of electromechanically active thin films (<1 µm) to enable micro- and nano-electromechanical systems. While the electromechanical response of such films is limited by substrate-induced mechanical clamping, large electromechanical responses in antiferroelectric and multilayer thin-film heterostructures have garnered interest. Here, multilayer thin-film heterostructures based on antiferroelectric PbHfO and ferroelectric PbHfTiO overcome substrate clamping to produce electromechanical strains >4.

Development and validation of the Parent-Reported Indicator of Developmental Evaluation for Chinese Children (PRIDE) tool.

Background: Developmental delay (DD) poses challenges to children's overall development, necessitating early detection and intervention. Existing screening tools in China focus mainly on children with developmental issues in two or more domains, diagnosed as global developmental delay (GDD). However, the recent rise of early childhood development (ECD) concepts has expanded the focus to include not only those with severe brain development impairments but also children who lag in specific domains due to various social-environmental factors, with the aim of promoting positive development through active intervention.

Variations in Prevalence and Characteristics of Rotavirus Diarrhea Among Outpatients - Shanghai Municipality, China, 2017-2023.

Introduction: This study investigated temporal changes in rotavirus group A (RVA) prevalence, epidemiological characteristics, and genotype distribution patterns among diarrhea outpatients in Shanghai Municipality, China.

Methods: We conducted prospective active surveillance of diarrheal disease in pediatric and adult outpatients in Shanghai. Stool specimens were analyzed for five viral and twelve bacterial pathogens.

Directional Mushroom-Derived Scaffold for Microenvironment Regulation in Infected Bone Defects.

Infected bone defects are a common clinical condition, but conventional treatments often fail to achieve the desired outcomes, including addressing antibiotic resistance and preventing nonunion complications. In the presented study, a functionalized decellularized mushroom stem scaffold is developed composed of its naturally aligned channels, Zn/curcumin MOFs, hydroxyapatite minerals, and icariin. In vitro, It is found that functionalized acellular mushroom stem scaffold can control bacterial infections through Zn/curcumin MOFs.

Protocol to Retrieve Unknown Flanking DNA Using Fork PCR for Genome Walking.

PCR-based genome walking is one of the prevalent techniques implemented to acquire unknown flanking genomic DNAs. The worth of genome walking includes but is not limited to cloning full-length genes, mining new genes, and discovering regulatory regions of genes. Therefore, this technique has advanced molecular biology and related fields.

M1 macrophage membrane-coated nickel-arsenic nanocomplex promoting synergistic treatment of hepatocellular carcinoma.

By inducing apoptosis, promoting differentiation and reducing the migration of cancer cells, arsenic has a higher therapeutic effect and lower risk of recurrence and metastasis than conventional anticancer drugs. However, the low bioavailability and adverse side effects of arsenic hinder its application in hepatocellular carcinoma (HCC). Therefore, a M1 macrophage membrane-coated nickel-arsenic/polydopamine nanocomplex (NiAsOx@P@M) was constructed to enhance the combined antitumor effects of chemotherapy and immunotherapy.

Rotavirus vaccine effectiveness and coverage among children younger than 5 years old in Shanghai, China: A test-negative case control study.

Objectives: The number of post-marketing studies assessing the vaccine effectiveness (VE) of the Lanzhou lamb rotavirus vaccine (LLR, licensed in 2000 exclusively in China) and the oral human attenuated pentavalent rotavirus vaccine (RotaTeq, licensed in China in 2018) in China is limited.

Methods: A test-negative case-control study based on prospective surveillance was conducted among diarrhea patients aged 5 years and younger at five hospitals in Shanghai, China. Cases and controls were defined based on the results of real-time fluorescent quantitative reverse transcription polymerase chain reaction (rRT-PCR) of fecal samples for rotavirus.

Regulating Intermediate Adsorption and Promoting Charge Transfer of CoCr-LDHs by Ce Doping for Enhancing Electrooxidation of 5-Hydroxymethylfurfural.

Electrochemical oxidation of 5-hydroxymethylfurfural (HMFOR) to generate high-value chemicals under mild conditions acts as an energy-saving and sustainable strategy. However, it is still challenging to develop electrocatalysts with high efficiency and good durability. Here, nickel foam (NF) supported CoCrCe(7.

Causal Relationship Between Circulating Metabolites and Sarcopenia-Related Traits: A Mendelian Randomization and Experimental Study.

Sarcopenia (SP), an age-associated condition marked by muscle weakness and loss has been strongly connected with metabolic factors according to substantial evidence. Nevertheless, the causal correlation between SP and serum metabolites, and the biological signaling pathways involved, is still not well understood. We performed a bidirectional two-sample Mendelian randomization (MR) analysis to examine the causal relationships between 1091 levels and 309 ratios of metabolites with SP traits, alongside investigating the relevant biological signaling pathways.

Phenylpropanoid dimers from Chrysanthemum indicum with antioxidant effects via activating the Nrf2 signaling pathway.

Five phenylpropanoid dimers chrysanthephenyls A-E, with the 1-O-1'-type (1) and 1-O-2'-type (2-5) ether bond linking modes, were isolated from the aerial parts of Chrysanthemum indicum. Among them, chrysanthephenyls D and E were two pairs of enantiomers, and chrysanthephenyl E was further resolved into its enantiomers (+)-chrysanthephenyl E and (-)-chrysanthephenyl E via chiral-phase HPLC. Their structures were characterized on the basic analysis of the spectroscopic techniques (IR, HRESIMS, 1D and 2D NMR), and their absolute configurations were determined by DP4+ NMR analysis and ECD calculation method.

Developmental trends and knowledge frameworks in the application of radiomics in prostate cancer: a bibliometric analysis from 2000 to 2024.

Background: This research utilized the bibliometrics method to analyze the published literature related to prostate cancer (PCa) imaging. Furthermore, current knowledge and research hotspots of radiomics in PCa diagnosis and treatment were comprehensively reviewed, as well as progress and emerging trends in field were explored.

Methods: In this investigation, the relevant literature on radiomics, and PCa was retrieved from Web of Science Core Collection (WoSCC) databases from 2000 and 2024.

Morphometric Study on the Mandible of Colobine Fossil (Mesopithecus pentelicus) Found in East Asia, a Comparison With Extant Taxa.

A tooth-bearing mandible fossil of a colobine monkey discovered at Shuitangba, Zhaotong, Yunnan, China, was morphometrically analyzed and compared with extant Asian colobines. Our previous qualitative and quantitative descriptions indicate that it can be safely attributed to Mesopithecus pentelicus, a Miocene fossil colobine widely found in Europe and South Asia. The present research aims to explore fossil association with extant colobines and functionally propose its dietary preferences based on multivariate morphometric analyses of mandibular morphology.

The value of signal intensity ratios of orbital tissue to white matter of orbital MRI in evaluating graves' orbitopathy.

Absract: PURPOSE: To investigate the signal intensity ratio (SIR) of orbital tissue to white matter in orbital magnetic resonance imaging (MRI) for assessing graves' orbitopathy (GO).

Methods: This study enrolled a total of 42 patients (79 eyes) with GO as the GO group, 10 patients (20 eyes) diagnosed with graves' disease (GD) without orbitopathy during the same period as the GD group, and 23 normal individuals with negative MRI results and no thyroid or eye diseases as the healthy control (HC) group. The signal intensity of the lacrimal gland (LG), extraocular muscle (EOM), ipsilateral temporal muscle (TM), ramus mandibulae, and white matter (WM) on MRI images was measured.

Chaihu-Shugan-San Ameliorated Osteoporosis of Mice with Depressive Behavior Caused by Chronic Unpredictable Mild Stress via Repressing Neuroinflammation and HPA Activity.

Objective: Depression and osteoporosis are usually concurrent health problems. This study aimed to explore the development of osteoporosis in depressive mice model and investigate the beneficial effects of the classical herbal formula Chaihu-Shugan-San (CHSG) on the brain and bone.

Methods: CHSG powder was prepared by spray-drying following extraction with water.

Comparison of 68Ga-FAPI PET CT/MRI and 18F-FDG PET/CT in metastatic lesions of gynecological cancers: a systematic review and head-to-head meta-analysis.

Background: 68Ga-labled fibroblast activating protein inhibitor (68Ga-FAPI) represents a new and exciting positron emission tomography-computed tomography/magnetic resonance (PET-CT/MR) radiotracer.

Purpose: To compare the diagnostic efficacy of 68Ga-FAPI PET CT/MR and 18F-fluorodeoxyglucose (18F-FDG) PET/CT in metastatic lesions of gynecological cancers (GCs).

Material And Methods: The PubMed, Embase, and Web of Science databases were thoroughly investigated from inception until 22 December 2023.

Ginsenoside reprogramming microglia through the FGF/FGFR1 inhibits post traumatic stress disorder.

Post traumatic stress disorder (PTSD) is a serious and persistent mental diseases. Nowadays, Treatment of PTSD patients in clinical practice is mainly based on drug therapy accompanied by psychological therapy. However, the therapeutic effect is unsatisfactory.

Characterization of the function of Adenovirus L4 gene products and their impact on AAV vector production.

Efficient manufacturing of recombinant adenovirus-associated virus (AAV) vectors is critical to the successful development of genomic medicines. We attempted to optimize AAV vector production in a producer cell line platform. In this system, helper functions required for AAV replication and production are provided via infection with a replication-competent wild-type Adenovirus.

[Water Transformation Relationship in Inner Mongolia Section of the Yellow River Basin Based on Stable Hydrogen and Oxygen Isotopes].

By collecting the atmospheric precipitation, surface water, and groundwater in the Inner Mongolia section of the Yellow River Basin in July 2021 （wet season）, October （normal season）, and April 2022 （dry season）, stable isotope technology was used to analyze the temporal and spatial changes in hydrogen and oxygen stable isotopes in the "three rivers" of the basin, and the MixSIAR mixing model was used to reveal the water body transformation relationship. The results showed that the mean difference in the groundwater isotope was small in the abundance period, flat period, and dry period in the Mongolia section of the Yellow River Basin. The groundwater regeneration was slow, the retention time was long, the seasonal variation was not obvious, and the D value of surface water was higher in the abundance period than in the normal period and dry period.

[Unilateral biportal endoscopic posterior cervical foraminotomy for cervical radiculopathy].

Objective: To investigate the clinical efficacy of posterior unilateral biportal endoscopic(UBE) cervical discectomy for cervical radiculopathy under general anesthesia.

Methods: A retrospective analysis of 35 patients with cervical disc herniation uderwent posterior UBE cervical discectomy under general anesthesia from March 2021 to March 2023 was performed, including 17 males and 18 females, with an average age of (56.00±7.