Effects of electron beam irradiation on proteins and exopolysaccharide production and changes in .

often threaten human health and safety for the toxin and unpleasant odor and removal difficulties during water treatment process. In order to remove it, a novel method high energy was studied in this research. The electron beam generated by an accelerator was applied to irradiate at various doses of 1, 2, 3, 4 and 5 kGy.

Separation and identification of antidepressants by acrylate-based monolithic column capillary electrochromatography with UV detection.

This paper details a method for separation and identification of six antidepressant compounds using an acrylate-based porous monolithic capillary. Detection takes place using CEC coupled with UV or mass spectrometric detection. The CEC-UV method provides an effective and efficient method for the separation and identification of the analytes.

A rapid method for separation and identification of microcystins using capillary electrophoresis and time-of-flight mass spectrometry.

This paper demonstrates a method for the rapid separation and identification of four microcystin (MC) variants commonly found in aquatic environments. The procedure utilizes capillary electrophoresis (CE) coupled to UV absorbance and time-of-flight mass spectrometric (TOF-MS) detectors. All four analytes were effectively separated within 6min using phosphate buffer in 50-μm ID capillaries with an applied electric field of 400V/cm.

Chiral separation of 12 cathinone analogs by cyclodextrin-assisted capillary electrophoresis with UV and mass spectrometry detection.

In this study, a rapid chiral separation of 12 cathinones analogs has been developed and validated using cyclodextrin-assisted CE with UV and TOF-MS detection. Optimum separation was obtained on a 57.5 cm × 50 μm capillary using a buffer system consisting of 10 mM β-cyclodextrin (β-CD) in a 100 mM phosphate buffer for CE-UV, and 0.

A novel fluorogenic coumarin substrate for monitoring acid phosphatase activity at low pH environment.

This article described the synthesis and application of 6-chloro-8-fluoro-4-methylumbelliferone phosphate (CF-MUP) in analyzing acid phosphatase activity. Compared to the existing MUP, the new coumarin phosphate, CF-MUP, demonstrateed much higher sensitivity and was more robust for detecting the activity of acid phosphatase than the classic substrate 4-methylumbelliferone phosphate (MUP). The product of enzyme reaction, 6-chloro-8-fluoro-4-methylumbelliferone (CF-MU) possesses strong fluorescence at approximately 450 nm with low pKa (4.